NPSR1 (NM_207173) Human Tagged ORF Clone

CAT#: RC221361

NPSR1 (Myc-DDK-tagged)-Human neuropeptide S receptor 1 (NPSR1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NPSR1
• Synonyms: ASRT2; GPR154; GPRA; NPSR; PGR14; VRR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221361 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCCAACTTCACAGAGGGCAGCTTCGATTCCAGTGGGACCGGGCAGACGCTGGATTCTTCCCCAG
  TGGCTTGCACTGAAACAGTGACTTTTACTGAAGTGGTGGAAGGAAAGGAATGGGGTTCCTTCTACTACTC
  CTTTAAGACTGAGCAATTGATAACTCTGTGGGTCCTCTTTGTTTTTACCATTGTTGGAAACTCCGTTGTG
  CTTTTTTCCACATGGAGGAGAAAGAAGAAGTCAAGAATGACCTTCTTTGTGACTCAGCTGGCCATCACAG
  ATTCTTTCACAGGACTGGTCAACATCTTGACAGATATTAATTGGCGATTCACTGGAGACTTCACGGCACC
  TGACCTGGTTTGCCGAGTGGTCCGCTATTTGCAGGTTGTGCTGCTCTACGCCTCTACCTACGTCCTGGTG
  TCCCTCAGCATAGACAGATACCATGCCATCGTCTACCCCATGAAGTTCCTTCAAGGAGAAAAGCAAGCCA
  GGGTCCTCATTGTGATCGCCTGGAGCCTGTCTTTTCTGTTCTCCATTCCCACCCTGATCATATTTGGGAA
  GAGGACACTGTCCAACGGTGAAGTGCAGTGCTGGGCCCTGTGGCCTGACGACTCCTACTGGACCCCATAC
  ATGACCATCGTGGCCTTCCTGGTGTACTTCATCCCTCTGACAATCATCAGCATCATGTATGGCATTGTGA
  TCCGAACTATTTGGATTAAAAGCAAAACCTACGAAACAGTGATTTCCAACTGCTCAGATGGGAAACTGTG
  CAGCAGCTATAACCGAGGACTCATCTCAAAGGCAAAAATCAAGGCTATCAAGTATAGCATCATCATCATT
  CTTGCCTTCATCTGCTGTTGGAGTCCATACTTCCTGTTTGACATTTTGGACAATTTCAACCTCCTTCCAG
  ACACCCAGGAGCGTTTCTATGCCTCTGTGATCATTCAGAACCTGCCAGCATTGAATAGTGCCATCAACCC
  CCTCATCTACTGTGTCTTCAGCAGCTCCATCTCTTTCCCCTGCAGGGTCATCCGTCTCCGTCAGCTCCAG
  GAGGCTGCGCTAATGCTCTGCCCTCAACGAGAGAACTGGAAGGGTACTTGGCCAGGTGTACCTTCCTGGG
  CTCTTCCAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221361 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPANFTEGSFDSSGTGQTLDSSPVACTETVTFTEVVEGKEWGSFYYSFKTEQLITLWVLFVFTIVGNSVV
  LFSTWRRKKKSRMTFFVTQLAITDSFTGLVNILTDINWRFTGDFTAPDLVCRVVRYLQVVLLYASTYVLV
  SLSIDRYHAIVYPMKFLQGEKQARVLIVIAWSLSFLFSIPTLIIFGKRTLSNGEVQCWALWPDDSYWTPY
  MTIVAFLVYFIPLTIISIMYGIVIRTIWIKSKTYETVISNCSDGKLCSSYNRGLISKAKIKAIKYSIIII
  LAFICCWSPYFLFDILDNFNLLPDTQERFYASVIIQNLPALNSAINPLIYCVFSSSISFPCRVIRLRQLQ
  EAALMLCPQRENWKGTWPGVPSWALPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207173
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207173.1, NP_997056.1
• RefSeq Size: 1410 bp
• RefSeq ORF: 1134 bp
• Locus ID: 387129
• Protein Families: Druggable Genome, Transmembrane
• MW: 43.1 kDa
• Gene Summary: This gene encodes a member of the vasopressin/oxytocin subfamily of G protein-coupled receptors. The encoded membrane protein acts as a receptor for neuropeptide S and affects a variety of cellular processes through its signaling. Increased expression of this gene in ciliated cells of the respiratory epithelium and in bronchial smooth muscle cells is associated with asthma. Polymorphisms in this gene have also been associated with asthma susceptibility, panic disorders, inflammatory bowel disease, and rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]