CD21 (CR2) (NM_001877) Human Tagged ORF Clone

CAT#: RC221379

CR2 (Myc-DDK-tagged)-Human complement component (3d/Epstein Barr virus) receptor 2 (CR2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY419676) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221379 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CR2 protein (Cat# TP321379). The protein was produced from HEK293T cells transfected with CR2 cDNA clone (Cat# RC221379) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CR2
• Synonyms: C3DR; CD21; CR; CVID7; SLEB9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221379 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCGCGGGCCTGCTCGGGGTTTTCTTGGCTCTCGTCGCACCGGGGGTCCTCGGGATTTCTTGTG
  GCTCTCCTCCGCCTATCCTAAATGGCCGGATTAGTTATTATTCTACCCCCATTGCTGTTGGTACCGTGAT
  AAGGTACAGTTGTTCAGGTACCTTCCGCCTCATTGGAGAAAAAAGTCTATTATGCATAACTAAAGACAAA
  GTGGATGGAACCTGGGATAAACCTGCTCCTAAATGTGAATATTTCAATAAATATTCTTCTTGCCCTGAGC
  CCATAGTACCAGGAGGATACAAAATTAGAGGCTCTACACCCTACAGACATGGTGATTCTGTGACATTTGC
  CTGTAAAACCAACTTCTCCATGAACGGAAACAAGTCTGTTTGGTGTCAAGCAAATAATATGTGGGGGCCG
  ACACGACTACCAACCTGTGTAAGTGTTTTCCCTCTCGAGTGTCCAGCACTTCCTATGATCCACAATGGAC
  ATCACACAAGTGAGAATGTTGGCTCCATTGCTCCAGGATTGTCTGTGACTTACAGCTGTGAATCTGGTTA
  CTTGCTTGTTGGAGAAAAGATCATTAACTGTTTGTCTTCGGGAAAATGGAGTGCTGTCCCCCCCACATGT
  GAAGAGGCACGCTGTAAATCTCTAGGACGATTTCCCAATGGGAAGGTAAAGGAGCCTCCAATTCTCCGGG
  TTGGTGTAACTGCAAACTTTTTCTGTGATGAAGGGTATCGACTGCAAGGCCCACCTTCTAGTCGGTGTGT
  AATTGCTGGACAGGGAGTTGCTTGGACCAAAATGCCAGTATGTGAAGAAATTTTTTGCCCATCACCTCCC
  CCTATTCTCAATGGAAGACATATAGGCAACTCACTAGCAAATGTCTCATATGGAAGCATAGTCACTTACA
  CTTGTGACCCGGACCCAGAGGAAGGAGTGAACTTCATCCTTATTGGAGAGAGCACTCTCCGTTGTACAGT
  TGATAGTCAGAAGACTGGGACCTGGAGTGGCCCTGCCCCACGCTGTGAACTTTCTACTTCTGCGGTTCAG
  TGTCCACATCCCCAGATCCTAAGAGGCCGAATGGTATCTGGGCAGAAAGATCGATATACCTATAACGACA
  CTGTGATATTTGCTTGCATGTTTGGCTTCACCTTGAAGGGCAGCAAGCAAATCCGATGCAATGCCCAAGG
  CACATGGGAGCCATCTGCACCAGTCTGTGAAAAGGAATGCCAGGCCCCTCCTAACATCCTCAATGGGCAA
  AAGGAAGATAGACACATGGTCCGCTTTGACCCTGGAACATCTATAAAATATAGCTGTAACCCTGGCTATG
  TGCTGGTGGGAGAAGAATCCATACAGTGTACCTCTGAGGGGGTGTGGACACCCCCTGTACCCCAATGCAA
  AGTGGCAGCGTGTGAAGCTACAGGAAGGCAACTCTTGACAAAACCCCAGCACCAATTTGTTAGACCAGAT
  GTCAACTCTTCTTGTGGTGAAGGGTACAAGTTAAGTGGGAGTGTTTATCAGGAGTGTCAAGGCACAATTC
  CTTGGTTTATGGAGATTCGTCTTTGTAAAGAAATCACCTGCCCACCACCCCCTGTTATCTACAATGGGGC
  ACACACCGGGAGTTCCTTAGAAGATTTTCCATATGGAACCACGGTCACTTACACATGTAACCCTGGGCCA
  GAAAGAGGAGTGGAATTCAGCCTCATTGGAGAGAGCACCATCCGTTGTACAAGCAATGATCAAGAAAGAG
  GCACCTGGAGTGGCCCTGCTCCCCTATGTAAACTTTCCCTCCTTGCTGTCCAGTGCTCACATGTCCATAT
  TGCAAATGGATACAAGATATCTGGCAAGGAAGCCCCATATTTCTACAATGACACTGTGACATTCAAGTGT
  TATAGTGGATTTACTTTGAAGGGCAATAGTCAGATTCGTTGCAAAGCTGATAACACCTGGGATCCTGAAA
  TACCAGTTTGTGAAAAAGAAACATGCCAGCATGTGAGACAGAGTCTTCAAGAACTTCCAGCTGGTTCACG
  TGTGGAGCTAGTTAATACGTCCTGCCAAGATGGGTACCAGTTGACTGGACATGCTTATCAGATGTGTCAA
  GATGCTGAAAATGGAATTTGGTTCAAAAAGATTCCACTTTGTAAAGTTATTCACTGTCACCCTCCACCAG
  TGATTGTCAATGGGAAGCACACAGGCATGATGGCAGAAAACTTTCTATATGGAAATGAAGTCTCTTATGA
  ATGTGACCAAGGATTCTATCTCCTGGGAGAGAAAAAATTGCAGTGCAGAAGTGATTCTAAAGGACATGGA
  TCTTGGAGCGGGCCTTCCCCACAGTGCTTACGATCTCCTCCTGTGACTCGCTGCCCTAATCCAGAAGTCA
  AACATGGGTACAAGCTCAATAAAACACATTCTGCATATTCCCACAATGACATAGTGTATGTTGACTGCAA
  TCCTGGCTTCATCATGAATGGTAGTCGCGTGATTAGGTGTCATACTGATAACACATGGGTGCCAGGTGTG
  CCAACTTGTATCAAAAAAGCCTTCATAGGGTGTCCACCTCCGCCTAAGACCCCTAACGGGAACCATACTG
  GTGGAAACATAGCTCGATTTTCTCCTGGAATGTCAATCCTGTACAGCTGTGACCAAGGCTACCTGCTGGT
  GGGAGAGGCACTCCTTCTTTGCACACATGAGGGAACCTGGAGCCAACCTGCCCCTCATTGTAAAGAGGTA
  AACTGTAGCTCACCAGCAGATATGGATGGAATCCAGAAAGGGCTGGAACCAAGGAAAATGTATCAGTATG
  GAGCTGTTGTAACTCTGGAGTGTGAAGATGGGTATATGCTGGAAGGCAGTCCCCAGAGCCAGTGCCAATC
  GGATCACCAATGGAACCCTCCCCTGGCGGTTTGCAGATCCCGTTCACTTGCTCCTGTCCTTTGTGGTATT
  GCTGCAGGTTTGATACTTCTTACCTTCTTGATTGTCATTACCTTATACGTGATATCAAAACACAGAGAAC
  GCAATTATTATACAGATACAAGCCAGAAAGAAGCTTTTCATTTAGAAGCACGAGAAGTATATTCTGTTGA
  TCCATACAACCCAGCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221379 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGAAGLLGVFLALVAPGVLGISCGSPPPILNGRISYYSTPIAVGTVIRYSCSGTFRLIGEKSLLCITKDK
  VDGTWDKPAPKCEYFNKYSSCPEPIVPGGYKIRGSTPYRHGDSVTFACKTNFSMNGNKSVWCQANNMWGP
  TRLPTCVSVFPLECPALPMIHNGHHTSENVGSIAPGLSVTYSCESGYLLVGEKIINCLSSGKWSAVPPTC
  EEARCKSLGRFPNGKVKEPPILRVGVTANFFCDEGYRLQGPPSSRCVIAGQGVAWTKMPVCEEIFCPSPP
  PILNGRHIGNSLANVSYGSIVTYTCDPDPEEGVNFILIGESTLRCTVDSQKTGTWSGPAPRCELSTSAVQ
  CPHPQILRGRMVSGQKDRYTYNDTVIFACMFGFTLKGSKQIRCNAQGTWEPSAPVCEKECQAPPNILNGQ
  KEDRHMVRFDPGTSIKYSCNPGYVLVGEESIQCTSEGVWTPPVPQCKVAACEATGRQLLTKPQHQFVRPD
  VNSSCGEGYKLSGSVYQECQGTIPWFMEIRLCKEITCPPPPVIYNGAHTGSSLEDFPYGTTVTYTCNPGP
  ERGVEFSLIGESTIRCTSNDQERGTWSGPAPLCKLSLLAVQCSHVHIANGYKISGKEAPYFYNDTVTFKC
  YSGFTLKGNSQIRCKADNTWDPEIPVCEKETCQHVRQSLQELPAGSRVELVNTSCQDGYQLTGHAYQMCQ
  DAENGIWFKKIPLCKVIHCHPPPVIVNGKHTGMMAENFLYGNEVSYECDQGFYLLGEKKLQCRSDSKGHG
  SWSGPSPQCLRSPPVTRCPNPEVKHGYKLNKTHSAYSHNDIVYVDCNPGFIMNGSRVIRCHTDNTWVPGV
  PTCIKKAFIGCPPPPKTPNGNHTGGNIARFSPGMSILYSCDQGYLLVGEALLLCTHEGTWSQPAPHCKEV
  NCSSPADMDGIQKGLEPRKMYQYGAVVTLECEDGYMLEGSPQSQCQSDHQWNPPLAVCRSRSLAPVLCGI
  AAGLILLTFLIVITLYVISKHRERNYYTDTSQKEAFHLEAREVYSVDPYNPAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001877
• ORF Size: 3099 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001877.1, NM_001877.2, NM_001877.3, NM_001877.4, NP_001868.2
• RefSeq Size: 4007 bp
• RefSeq ORF: 3102 bp
• Locus ID: 1380
• Cytogenetics: 1q32.2
• Domains: CCP
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Complement and coagulation cascades, Hematopoietic cell lineage
• MW: 113 kDa
• Gene Summary: 'This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]'