EYS (NM_198283) Human Tagged ORF Clone

CAT#: RC221392

EYS (Myc-DDK-tagged)-Human eyes shut homolog (Drosophila) (EYS), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY405007) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221392 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EYS
• Synonyms: bA74E24.1; bA166P24.2; bA307F22.3; C6orf178; C6orf179; C6orf180; dJ22I17.2; dJ303F19.1; dJ1018A4.2; EGFL10; EGFL11; RP25; SPAM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221392 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGACAAATCAATCGTCATTCTGAGCCTGATGGTTTTTCACAGCTCTTTCATAAATGGAAAAACAT
  GTAGACGGCAATTGGTGGAAGAATGGCATCCACAACCCTCATCATATGTGGTAAATTGGACACTAACAGA
  AAACATCTGCTTGGACTTCTACAGAGATTGCTGGTTTTTGGGTGTAAACACTAAAATAGATACTTCAGGC
  AATCAAGCTGTTCCCCAGATTTGCCCTTTGCAAATTCAATTAGGAGATATCCTTGTAATTTCTTCTGAAC
  CATCTCTTCAATTTCCAGAAATAAATTTGATGAATGTTTCTGAAACATCTTTCGTTGGCTGTGTGCAAAA
  TACCACAATGGAAGATCAGTTACTTTTTGGCTGCAGACTAAAAGGAATGCACACTGTTAATTCTAAGTGG
  CTGAGTGTTGGGACACATTATTTTATCACAGTTATGGCAAGTGGTCCATCACCTTGTCCACTGGGACTTC
  GACTAAATGTGACAGTGAAACAGCAGTTCTGCCAGGAATCTCTGAGTTCAGAATTTTGCTCTGGTCATGG
  TAAATGTCTTAGTGAAGCTTGGAGCAAGACATATAGCTGCCATTGCCAGCCTCCATTTTCTGGAAAATAC
  TGCCAGGAACTTGATGCATGTTCTTTTAAACCATGTAAAAATAATGGCAGTTGCATTAATAAAAGAGAAA
  ATTGGGATGAGCAAGCATATGAATGTGTCTGTCACCCACCATTTACAGGAAAGAATTGCTCAGAAATAAT
  TGGCCAGTGTCAACCACATGTCTGTTTCCATGGAAACTGCAGCAATATTACTTCAAATAGTTTCATTTGT
  GAATGTGATGAGCAATTTTCAGGTCCATTCTGTGAGGTGTCAGCAAAACCTTGTGTTTCTCTGCTTTTTT
  GGAAAAGAGGAATTTGCCCAAATAGCAGTTCTGCTTATACTTATGAATGCCCAAAAGGATCTTCCAGCCA
  AAATGGTGAAACTGATGTCAGTGAGTTTTCATTAGTACCATGTCAGAATGGTACTGACTGCATCAAAATA
  TCAAATGATGTTATGTGCATCTGTTCACCAATATTTACAGATTTGCTTTGTAAGAGCATTCAAACATCAT
  GTGAGTCATTTCCTTTGAGGAATAACGCTACATGTAAGAAATGTGAGAAAGATTATCCTTGCAGCTGTAT
  ATCAGGATTTACTGAAAAAAACTGTGAGAAAGCAATTGACCACTGTAAACTGCTCAGCATCAACTGTCTG
  AATGAAGAATGGTGTTTCAATATAATTGGAAGATTCAAATATGTATGCATTCCAGGGTGCACAAAAAATC
  CATGTTGGTTTTTGAAGAATGTTTACCTAATTCATCAACACCTCTGCTACTGTGGAGTCACCTTCCATGG
  TATTTGCCAAGATAAAGGTCCTGCTCAATTTGAATATGTGTGGCAATTGGGATTTGCAGGATCTGAAGGC
  GAAAAGTGCCAAGGGGTTATTGATGCCTATTTCTTTCTGGCTGCAAACTGCACTGAAGATGCAACCTATG
  TGAACGATCCTGAAGATAATAATTCTTCATGTTGGTTCCCACATGAAGGCACAAAAGAGATTTGTGCAAA
  TGGATGCAGTTGTTTGAGTGAAGAAGACAGTCAGGAATATCGGTATCTATGTTTTCTCAGATGGGCTGGC
  AACATGTATCTGGAAAATACAACTGATGATCAAGAAAATGAGTGTCAACATGAAGCTGTTTGTAAAGATG
  AAATTAATAGACCCAGGTATTTATGTATCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221392 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTDKSIVILSLMVFHSSFINGKTCRRQLVEEWHPQPSSYVVNWTLTENICLDFYRDCWFLGVNTKIDTSG
  NQAVPQICPLQIQLGDILVISSEPSLQFPEINLMNVSETSFVGCVQNTTMEDQLLFGCRLKGMHTVNSKW
  LSVGTHYFITVMASGPSPCPLGLRLNVTVKQQFCQESLSSEFCSGHGKCLSEAWSKTYSCHCQPPFSGKY
  CQELDACSFKPCKNNGSCINKRENWDEQAYECVCHPPFTGKNCSEIIGQCQPHVCFHGNCSNITSNSFIC
  ECDEQFSGPFCEVSAKPCVSLLFWKRGICPNSSSAYTYECPKGSSSQNGETDVSEFSLVPCQNGTDCIKI
  SNDVMCICSPIFTDLLCKSIQTSCESFPLRNNATCKKCEKDYPCSCISGFTEKNCEKAIDHCKLLSINCL
  NEEWCFNIIGRFKYVCIPGCTKNPCWFLKNVYLIHQHLCYCGVTFHGICQDKGPAQFEYVWQLGFAGSEG
  EKCQGVIDAYFFLAANCTEDATYVNDPEDNNSSCWFPHEGTKEICANGCSCLSEEDSQEYRYLCFLRWAG
  NMYLENTTDDQENECQHEAVCKDEINRPRYLCII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198283
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198283.1, NP_938024.1
• RefSeq Size: 2245 bp
• RefSeq ORF: 1785 bp
• Locus ID: 346007
• Protein Families: Druggable Genome
• MW: 66.9 kDa
• Gene Summary: The product of this gene contains multiple epidermal growth factor (EGF)-like and LamG domains. The protein is expressed in the photoreceptor layer of the retina, and the gene is mutated in autosomal recessive retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]