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PTPRS (NM_002850) Human Tagged ORF Clone
CAT#: RC221440
- TrueORF®
PTPRS (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, S (PTPRS), transcript variant 1
"NM_002850" in other vectors (2)
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | PTPRS |
| Synonyms | PTPSIGMA; R-PTP-S; R-PTP-sigma |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC221440 representing NM_002850.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGCCCACCTGGGGCCCTGGCATGGTGTCTGTGGTTGGTCCCATGGGCCTCCTTGTGGTCCTGCTC GTTGGAGGCTGTGCAGCAGAAGAGCCCCCCAGGTTTATCAAAGAACCCAAGGACCAGATCGGCGTGTCG GGGGGTGTGGCCTCTTTCGTGTGTCAGGCCACGGGTGACCCCAAGCCACGAGTGACCTGGAACAAGAAG GGCAAGAAGGTCAACTCTCAGCGCTTTGAGACGATTGAGTTTGATGAGAGTGCAGGGGCAGTGCTGAGG ATCCAGCCGCTGAGGACACCGCGGGATGAAAACGTGTACGAGTGTGTGGCCCAGAACTCGGTTGGGGAG ATCACAGTCCATGCCAAGCTTACTGTCCTCCGAGAGGACCAGCTGCCCTCTGGCTTCCCCAACATCGAC ATGGGCCCACAGTTGAAGGTGGTGGAGCGGACACGGACAGCCACCATGCTCTGTGCAGCCAGCGGCAAC CCTGACCCTGAGATCACCTGGTTCAAGGACTTCCTGCCTGTGGATCCTAGTGCCAGCAATGGACGCATC AAACAGCTGCGATCAGAAACCTTTGAAAGCACTCCGATTCGAGGAGCCCTGCAGATTGAAAGCAGTGAG GAAACCGACCAGGGCAAATATGAGTGTGTGGCCACCAACAGCGCCGGCGTGCGCTACTCCTCACCTGCC AACCTCTACGTGCGAGAGCTTCGAGAAGTCCGCCGCGTGGCCCCGCGCTTCTCCATCCTGCCCATGAGC CACGAGATCATGCCAGGGGGCAACGTGAACATCACCTGCGTGGCCGTGGGCTCGCCCATGCCATACGTG AAGTGGATGCAGGGGGCCGAGGACCTGACCCCCGAGGATGACATGCCCGTGGGTCGGAACGTGCTGGAA CTCACAGATGTCAAGGACTCGGCCAACTACACCTGCGTGGCCATGTCCAGCCTGGGCGTCATTGAGGCG GTTGCTCAGATCACGGTGAAATCTCTCCCCAAAGCTCCCGGGACTCCCATGGTGACTGAGAACACAGCC ACCAGCATCACCATCACGTGGGACTCGGGCAACCCAGATCCTGTGTCCTATTACGTCATCGAATATAAA TCCAAGAGCCAAGACGGGCCGTATCAGATTAAAGAGGACATCACCACCACACGTTACAGCATCGGCGGC CTGAGCCCCAACTCGGAGTACGAGATCTGGGTGTCGGCCGTCAACTCCATCGGCCAGGGGCCCCCCAGC GAGTCCGTGGTCACCCGCACAGGCGAGCAGGCCCCGGCCAGCGCGCCGCGGAACGTGCAAGCCCGGATG CTCAGCGCGACCACCATGATTGTGCAGTGGGAGGAGCCGGTGGAGCCCAACGGCCTGATCCGCGGCTAC CGCGTCTACTACACCATGGAACCGGAGCACCCCGTGGGCAACTGGCAGAAGCACAACGTGGACGACAGC CTGCTGACCACCGTGGGCAGCCTGCTGGAGGACGAGACCTACACCGTGCGGGTGCTCGCCTTCACCTCC GTCGGCGACGGGCCCCTCTCGGACCCCATCCAGGTCAAGACGCAGCAGGGAGTGCCGGGCCAGCCCATG AACCTGCGGGCCGAGGCCAGGTCGGAGACCAGCATCACGCTGTCCTGGAGCCCCCCGCGGCAGGAGAGT ATCATCAAGTACGAGCTCCTCTTCCGGGAAGGCGACCATGGCCGGGAGGTGGGAAGGACCTTCGACCCG ACGACTTCCTACGTGGTGGAGGACCTGAAGCCCAACACGGAGTACGCCTTCCGCCTGGCGGCCCGCTCG CCGCAGGGCCTGGGCGCCTTCACCCCCGTGGTGCGGCAGCGCACGCTGCAGTCCAAACCGTCAGCCCCC CCTCAAGACGTTAAATGTGTCAGCGTGCGCTCCACGGCCATTTTGGTAAGTTGGCGCCCGCCGCCGCCG GAAACGCACAACGGGGCCCTGGTGGGCTACAGCGTCCGCTACCGACCGCTGGGCTCAGAGGACCCGGAA CCCAAGGAGGTGAACGGCATCCCCCCGACCACCACTCAGATCCTGCTGGAGGCCTTGGAGAAGTGGACC CAGTACCGCATCACGACTGTCGCTCACACAGAGGTGGGACCAGGGCCCGAGAGCTCGCCCGTGGTCGTC CGCACCGACGAGGATGTGCCCAGCGCGCCGCCGCGGAAGGTGGAGGCGGAGGCGCTCAACGCCACGGCC ATCCGCGTGCTGTGGCGCTCGCCCGCGCCCGGCCGGCAGCACGGCCAGATCCGCGGCTACCAGGTCCAC TACGTGCGCATGGAGGGCGCCGAGGCCCGCGGGCCGCCGCGCATCAAGGACGTCATGCTGGCCGATGCC CAGTGGGAGACGGATGACACGGCCGAATATGAGATGGTCATCACAAACTTGCAGCCTGAGACCGCGTAC TCCATCACGGTAGCCGCCTACACCATGAAGGGCGATGGCGCTCGCAGCAAACCCAAGGTGGTTGTGACC AAGGGAGCAGTGCTGGGCCGCCCAACCCTGTCGGTGCAGCAGACCCCCGAGGGCAGCCTGCTGGCACGC TGGGAGCCCCCGGCTGGCACCGCGGAGGACCAGGTGCTGGGCTACCGCCTGCAGTTTGGCCGTGAGGAC TCGACGCCCCTGGCCACCCTGGAGTTCCCGCCCTCCGAGGACCGCTACACGGCATCAGGCGTGCACAAG GGGGCCACGTATGTGTTCCGGCTTGCGGCCCGGAGCCGCGGCGGCCTGGGCGAGGAGGCAGCCGAGGTC CTGAGCATCCCGGAGGACACGCCCCGTGGCCACCCGCAGATTCTGGAGGCGGCCGGCAACGCCTCGGCC GGGACCGTCCTTCTCCGCTGGCTGCCACCCGTGCCCGCCGAGCGCAACGGGGCCATCGTCAAATACACG GTGGCCGTGCGGGAGGCCGGTGCCCTGGGCCCTGCCCGAGAGACTGAGCTGCCGGCAGCGGCTGAGCCG GGCGCGGAGAACGCGCTCACGCTGCAGGGCCTGAAGCCCGACACGGCCTATGACCTCCAAGTGCGAGCC CACACGCGCCGGGGCCCTGGCCCCTTCAGCCCCCCCGTCCGCTACCGGACGTTCCTGCGGGACCAAGTC TCGCCCAAGAACTTCAAGGTGAAAATGATCATGAAGACATCAGTTCTGCTCAGCTGGGAGTTCCCTGAC AACTACAACTCACCCACACCCTACAAGATCCAGTACAATGGGCTCACACTGGATGTGGATGGCCGTACC ACCAAGAAGCTCATCACGCACCTCAAGCCCCACACCTTCTACAACTTTGTGCTGACCAATCGCGGCAGC AGCCTGGGCGGCCTCCAGCAGACGGTCACCGCCTGGACTGCCTTCAACCTGCTCAACGGCAAGCCCAGC GTCGCCCCCAAGCCTGATGCTGACGGCTTCATCATGGTGTATCTTCCTGACGGCCAGAGCCCCGTGCCT GTCCAGAGCTATTTCATTGTGATGGTGCCACTGCGCAAGTCTCGTGGAGGCCAATTCCTGACCCCGCTG GGTAGCCCAGAGGACATGGATCTGGAAGAGCTCATCCAGGACATCTCACGGCTACAGAGGCGCAGCCTG CGGCACTCGCGTCAGCTGGAGGTGCCCCGGCCCTATATTGCAGCTCGCTTCTCTGTGCTGCCACCCACG TTCCATCCCGGCGACCAGAAGCAGTATGGCGGCTTCGATAACCGGGGCCTGGAGCCCGGCCACCGCTAT GTCCTCTTCGTGCTTGCCGTGCTTCAGAAGAGCGAGCCTACCTTTGCAGCCAGTCCCTTCTCAGACCCC TTCCAGCTGGATAACCCGGACCCCCAGCCCATCGTGGATGGCGAGGAGGGGCTTATCTGGGTGATCGGG CCTGTGCTGGCCGTGGTCTTCATAATCTGCATTGTCATTGCTATCCTGCTCTACAAGAACAAACCCGAC AGTAAACGCAAGGACTCAGAACCCCGCACCAAATGCCTCCTGAACAATGCCGACCTCGCCCCTCACCAC CCCAAGGACCCTGTGGAAATGAGACGCATTAACTTCCAGACTCCAGATTCAGGCCTCAGGAGCCCCCTC AGGGAGCCGGGGTTTCACTTTGAAAGCATGCTTAGCCACCCGCCAATTCCCATCGCAGACATGGCGGAG CACACGGAGCGGCTCAAGGCCAACGACAGCCTCAAGCTCTCCCAGGAGTATGAGTCCATCGACCCTGGA CAGCAGTTCACATGGGAACATTCCAACCTGGAAGTGAACAAGCCGAAGAACCGCTATGCCAACGTCATC GCCTATGACCACTCCCGTGTCATCCTCCAGCCCATTGAAGGCATCATGGGCAGTGATTACATCAATGCC AACTACGTGGACGGCTACCGGTGTCAGAACGCGTACATTGCCACGCAGGGGCCGCTGCCTGAGACCTTT GGGGACTTCTGGCGTATGGTGTGGGAGCAGCGGTCGGCGACCATCGTCATGATGACGCGGCTGGAGGAG AAGTCACGGATCAAGTGTGATCAGTATTGGCCCAACAGAGGCACGGAGACCTACGGCTTCATCCAGGTC ACGTTGCTAGATACCATCGAGCTGGCCACATTCTGCGTCAGGACATTCTCTCTGCACAAGAATGGCTCC AGTGAGAAACGCGAGGTCCGCCAGTTCCAGTTTACGGCGTGGCCGGACCATGGCGTGCCCGAATACCCA ACGCCCTTCCTGGCTTTCCTGCGGAGAGTCAAGACCTGCAACCCGCCAGATGCCGGCCCCATCGTGGTT CACTGCAGTGCCGGTGTGGGCCGCACAGGCTGCTTTATCGTCATCGACGCCATGCTTGAGCGGATCAAG CCAGAGAAGACAGTCGATGTCTATGGCCACGTGACGCTCATGAGGTCCCAGCGCAACTACATGGTGCAG ACGGAGGACCAGTACAGCTTCATCCACGAGGCCCTGCTGGAGGCCGTGGGCTGTGGCAACACAGAAGTG CCCGCACGCAGCCTCTATGCCTACATCCAGAAGCTGGCCCAGGTGGAGCCTGGCGAACACGTCACTGGC ATGGAACTCGAGTTCAAGCGGCTGGCTAACTCCAAGGCCCACACGTCACGCTTCATCAGTGCCAATCTG CCTTGTAACAAGTTCAAGAACCGCCTGGTGAACATCATGCCCTATGAGAGCACACGGGTCTGTCTGCAA CCCATCCGGGGTGTGGAGGGCTCTGACTACATCAACGCCAGCTTCATTGATGGCTACAGGCAGCAGAAG GCCTACATCGCGACACAGGGGCCGCTGGCGGAGACCACGGAAGACTTCTGGCGCATGCTGTGGGAGAAC AATTCGACGATCGTGGTGATGCTGACCAAGCTGCGGGAGATGGGCCGGGAGAAGTGTCACCAGTACTGG CCGGCCGAGCGCTCTGCCCGCTACCAGTACTTTGTGGTAGATCCGATGGCAGAATACAACATGCCTCAG TATATCCTGCGAGAGTTCAAGGTCACAGATGCCCGGGATGGCCAGTCCCGGACTGTCCGGCAGTTCCAG TTCACAGACTGGCCGGAACAGGGTGTGCCAAAGTCGGGGGAGGGCTTCATCGACTTCATTGGCCAAGTG CATAAGACTAAGGAGCAGTTTGGCCAGGACGGCCCCATCTCTGTCCACTGCAGTGCCGGCGTGGGCAGG ACGGGCGTCTTCATCACGCTTAGCATCGTGCTGGAGCGGATGCGGTATGAAGGCGTGGTGGACATCTTT CAGACGGTGAAGATGCTACGAACCCAGCGGCCGGCCATGGTGCAGACAGAGGATGAGTACCAGTTCTGT TACCAGGCGGCACTGGAGTACCTCGGAAGCTTTGACCACTATGCAACC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA >Peptide sequence encoded by RC221440
Blue=ORF Red=Cloning site Green=Tag(s) MAPTWGPGMVSVVGPMGLLVVLLVGGCAAEEPPRFIKEPKDQIGVSGGVASFVCQATGDPKPRVTWNKK GKKVNSQRFETIEFDESAGAVLRIQPLRTPRDENVYECVAQNSVGEITVHAKLTVLREDQLPSGFPNID MGPQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDPSASNGRIKQLRSETFESTPIRGALQIESSE ETDQGKYECVATNSAGVRYSSPANLYVRELREVRRVAPRFSILPMSHEIMPGGNVNITCVAVGSPMPYV KWMQGAEDLTPEDDMPVGRNVLELTDVKDSANYTCVAMSSLGVIEAVAQITVKSLPKAPGTPMVTENTA TSITITWDSGNPDPVSYYVIEYKSKSQDGPYQIKEDITTTRYSIGGLSPNSEYEIWVSAVNSIGQGPPS ESVVTRTGEQAPASAPRNVQARMLSATTMIVQWEEPVEPNGLIRGYRVYYTMEPEHPVGNWQKHNVDDS LLTTVGSLLEDETYTVRVLAFTSVGDGPLSDPIQVKTQQGVPGQPMNLRAEARSETSITLSWSPPRQES IIKYELLFREGDHGREVGRTFDPTTSYVVEDLKPNTEYAFRLAARSPQGLGAFTPVVRQRTLQSKPSAP PQDVKCVSVRSTAILVSWRPPPPETHNGALVGYSVRYRPLGSEDPEPKEVNGIPPTTTQILLEALEKWT QYRITTVAHTEVGPGPESSPVVVRTDEDVPSAPPRKVEAEALNATAIRVLWRSPAPGRQHGQIRGYQVH YVRMEGAEARGPPRIKDVMLADAQWETDDTAEYEMVITNLQPETAYSITVAAYTMKGDGARSKPKVVVT KGAVLGRPTLSVQQTPEGSLLARWEPPAGTAEDQVLGYRLQFGREDSTPLATLEFPPSEDRYTASGVHK GATYVFRLAARSRGGLGEEAAEVLSIPEDTPRGHPQILEAAGNASAGTVLLRWLPPVPAERNGAIVKYT VAVREAGALGPARETELPAAAEPGAENALTLQGLKPDTAYDLQVRAHTRRGPGPFSPPVRYRTFLRDQV SPKNFKVKMIMKTSVLLSWEFPDNYNSPTPYKIQYNGLTLDVDGRTTKKLITHLKPHTFYNFVLTNRGS SLGGLQQTVTAWTAFNLLNGKPSVAPKPDADGFIMVYLPDGQSPVPVQSYFIVMVPLRKSRGGQFLTPL GSPEDMDLEELIQDISRLQRRSLRHSRQLEVPRPYIAARFSVLPPTFHPGDQKQYGGFDNRGLEPGHRY VLFVLAVLQKSEPTFAASPFSDPFQLDNPDPQPIVDGEEGLIWVIGPVLAVVFIICIVIAILLYKNKPD SKRKDSEPRTKCLLNNADLAPHHPKDPVEMRRINFQTPDSGLRSPLREPGFHFESMLSHPPIPIADMAE HTERLKANDSLKLSQEYESIDPGQQFTWEHSNLEVNKPKNRYANVIAYDHSRVILQPIEGIMGSDYINA NYVDGYRCQNAYIATQGPLPETFGDFWRMVWEQRSATIVMMTRLEEKSRIKCDQYWPNRGTETYGFIQV TLLDTIELATFCVRTFSLHKNGSSEKREVRQFQFTAWPDHGVPEYPTPFLAFLRRVKTCNPPDAGPIVV HCSAGVGRTGCFIVIDAMLERIKPEKTVDVYGHVTLMRSQRNYMVQTEDQYSFIHEALLEAVGCGNTEV PARSLYAYIQKLAQVEPGEHVTGMELEFKRLANSKAHTSRFISANLPCNKFKNRLVNIMPYESTRVCLQ PIRGVEGSDYINASFIDGYRQQKAYIATQGPLAETTEDFWRMLWENNSTIVVMLTKLREMGREKCHQYW PAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTVRQFQFTDWPEQGVPKSGEGFIDFIGQV HKTKEQFGQDGPISVHCSAGVGRTGVFITLSIVLERMRYEGVVDIFQTVKMLRTQRPAMVQTEDEYQFC YQAALEYLGSFDHYAT SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002850 |
| ORF Size | 5844 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002850.4 |
| RefSeq Size | 7347 bp |
| RefSeq ORF | 5847 bp |
| Locus ID | 5802 |
| UniProt ID | Q13332 |
| Cytogenetics | 19p13.3 |
| Protein Families | Druggable Genome, Phosphatase, Transmembrane |
| MW | 217 kDa |
| Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of multiple Ig-like and fibronectin type III-like domains. Studies of the similar gene in mice suggested that this PTP may be involved in cell-cell interaction, primary axonogenesis, and axon guidance during embryogenesis. This PTP has been also implicated in the molecular control of adult nerve repair. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008] |
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