CNTNAP5 (NM_130773) Human Tagged ORF Clone

CAT#: RC221472

CNTNAP5 (Myc-DDK-tagged)-Human contactin associated protein-like 5 (CNTNAP5)

Product Images

Western blot validation of overexpression lysate (Cat# LY408936) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221472 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CNTNAP5 protein (Cat# TP321472). The protein was produced from HEK293T cells transfected with CNTNAP5 cDNA clone (Cat# RC221472) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNTNAP5
• Synonyms: caspr5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221472 representing NM_130773
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCTTTACCACGGCTGACCAGCGTTTTGACTTTGCTGTTCTCTGGCTTGTGGCATTTAGGATTAA
  CAGCGACAAACTACAACTGTGATGATCCACTAGCATCCCTGCTCTCTCCAATGGCTTTTTCCAGTTCCTC
  AGACCTCACTGGCACTCACAGCCCAGCTCAACTCAACTGGAGAGTTGGAACTGGCGGTTGGTCCCCAGCA
  GATTCCAATGCTCAACAGTGGCTCCAGATGGACCTGGGAAACAGAGTAGAGATTACAGCAGTGGCCACGC
  AGGGAAGATACGGAAGCTCTGACTGGGTGACGAGTTACAGCCTGATGTTCAGTGACACAGGACGCAACTG
  GAAACAGTACAAACAAGAAGACAGCATCTGGACCTTTGCAGGAAACATGAATGCTGACAGCGTGGTGCAC
  CACAAGCTATTGCACTCAGTGAGAGCCCGATTTGTTCGCTTTGTGCCCCTGGAATGGAATCCCAGTGGGA
  AGATTGGCATGAGAGTCGAGGTCTACGGATGTTCCTATAAATCAGATGTTGCTGACTTTGATGGCCGAAG
  CTCACTTCTGTACAGGTTCAATCAGAAGTTGATGAGTACTCTCAAAGATGTGATCTCCCTGAAGTTCAAG
  AGCATGCAAGGAGATGGGGTCCTGTTCCATGGAGAAGGTCAGCGTGGAGACCACATCACCTTGGAACTCC
  AGAAGGGGAGGCTCGCCCTACACCTCAATTTGGGTGACAGCAAAGCGCGGCTCAGCAGCAGCTTGCCCTC
  TGCCACCCTGGGCAGCCTCCTGGATGACCAGCACTGGCACTCGGTCCTCATTGAGCGGGTGGGCAAGCAG
  GTGAACTTCACGGTGGACAAGCACACACAGCACTTCCGCACCAAGGGCGAGACGGATGCCTTAGACATTG
  ACTATGAGCTTAGTTTTGGAGGAATTCCAGTACCAGGAAAACCTGGGACCTTTTTAAAGAAAAACTTCCA
  TGGATGCATCGAAAACCTTTACTACAATGGAGTAAACATAATTGACCTGGCTAAGAGACGAAAGCATCAG
  ATCTATACTGGCAATGTCACTTTTTCCTGCTCCGAACCACAGATTGTGCCCATCACATTTGTCAACTCCA
  GCGGCAGCTATTTGCTGCTGCCCGGCACCCCCCAAATTGATGGGCTCTCAGTGAGTTTCCAGTTTCGAAC
  ATGGAACAAGGATGGTCTGCTTCTGTCCACAGAGCTGTCTGAGGGCTCGGGAACCCTGCTGCTGAGCCTG
  GAGGGTGGAATCCTGAGACTCGTGATTCAGAAAATGACAGAACGCGTAGCTGAAATCCTCACAGGCAGCA
  ACTTGAATGATGGCCTGTGGCACTCGGTTAGCATCAACGCCAGGAGGAACCGCATCACGCTCACTCTGGA
  TGATGAAGCAGCACCCCCGGCTCCAGACAGCACTTGGGTGCAGATTTATTCTGGAAATAGCTACTATTTT
  GGAGGGTGCCCCGACAATCTCACCGATTCCCAATGTTTAAATCCCATTAAGGCTTTCCAAGGCTGCATGA
  GGCTCATCTTTATTGATAACCAGCCCAAGGACCTCATTTCAGTTCAGCAAGGTTCCCTGGGGAATTTTAG
  TGATTTACACATTGATCTGTGTAGCATCAAAGACAGGTGTTTGCCAAACTACTGTGAACATGGAGGAAGC
  TGCTCCCAGTCCTGGACTACCTTCTATTGTAACTGCAGTGACACAAGTTACACTGGTGCCACCTGCCACA
  ACTCCATCTACGAGCAATCCTGCGAGGTGTACAGGCACCAGGGGAATACAGCCGGCTTCTTCTACATCGA
  CTCAGATGGCAGCGGCCCACTGGGACCTCTCCAGGTGTACTGCAATATCACTGAGGACAAGATCTGGACA
  TCAGTGCAGCACAACAATACAGAGCTGACCCGAGTGCGGGGCGCTAACCCTGAGAAGCCCTATGCCATGG
  CCTTGGACTACGGGGGCAGCATGGAACAGCTGGAGGCCGTGATCGACGGCTCTGAGCACTGTGAGCAGGA
  GGTGGCCTACCACTGCAGGAGGTCCCGCCTGCTCAACACGCCGGATGGAACACCATTTACCTGGTGGATT
  GGGCGGTCCAATGAAAGGCACCCTTACTGGGGAGGTTCCCCTCCTGGGGTCCAGCAGTGTGAGTGTGGCC
  TAGACGAGAGCTGCCTGGACATTCAGCACTTTTGCAATTGCGACGCTGACAAGGATGAATGGACAAATGA
  TACTGGCTTTCTTTCCTTCAAAGACCACTTGCCTGTCACTCAGATAGTTATCACTGATACCGACAGATCA
  AACTCAGAAGCCGCTTGGAGAATTGGTCCCTTGCGTTGCTATGGTGACCGACGCTTCTGGAACGCCGTCT
  CATTTTATACAGAAGCCTCTTACCTCCACTTTCCTACCTTCCATGCGGAATTCAGTGCCGATATTTCCTT
  CTTTTTTAAAACCACAGCATTATCCGGAGTTTTCCTAGAAAATCTTGGCATTAAAGACTTCATTCGACTC
  GAAATAAGCTCTCCTTCAGAGATCACCTTTGCCATCGATGTTGGGAATGGTCCTGTGGAGCTTGTAGTCC
  AGTCTCCTTCTCTTCTGAATGACAACCAATGGCACTATGTCCGGGCTGAGAGGAACCTCAAGGAGACCTC
  CCTGCAGGTGGACAACCTTCCAAGGAGCACCAGGGAGACGTCGGAGGAGGGCCATTTTCGACTGCAGCTG
  AACAGCCAGTTGTTTGTAGGGGGAACGTCATCCAGACAGAAAGGCTTCCTAGGATGCATTCGCTCCTTAC
  ACTTGAATGGACAGAAAATGGACCTGGAAGAGAGGGCAAAGGTCACATCTGGAGTCAGGCCAGGCTGCCC
  CGGCCACTGCAGCAGCTACGGCAGCATCTGCCACAACGGGGGCAAGTGTGTGGAGAAGCACAATGGCTAC
  CTGTGTGATTGCACCAATTCACCTTATGAAGGGCCCTTTTGCAAAAAAGAGGTTTCTGCTGTTTTTGAGG
  CTGGCACGTCGGTTACTTACATGTTTCAAGAACCCTATCCTGTGACCAAGAATATAAGCCTCTCATCCTC
  AGCTATTTACACAGATTCAGCTCCATCCAAGGAAAACATTGCACTTAGCTTTGTGACAACCCAGGCACCC
  AGTCTTTTGCTCTTTATCAATTCTTCTTCTCAGGACTTCGTGGTTGTTCTGCTCTGCAAGAATGGAAGCT
  TACAGGTTCGCTATCACCTAAACAAGGAAGAAACCCATGTATTCACCATTGATGCAGATAACTTTGCTAA
  CAGAAGGATGCACCACTTGAAGATTAACCGAGAGGGAAGAGAGCTTACCATTCAGATGGACCAGCAACTT
  CGACTCAGTTATAACTTCTCTCCGGAAGTAGAGTTCAGGGTTATAAGGTCACTCACCTTGGGCAAAGTCA
  CAGAGAATCTTGGTTTGGATTCTGAAGTTGCTAAAGCAAATGCCATGGGTTTTGCTGGATGCATGTCTTC
  CGTCCAGTACAACCACATAGCACCACTGAAGGCTGCCCTGCGCCATGCCACTGTCGCGCCTGTGACTGTC
  CATGGGACCTTGACGGAATCCAGCTGTGGCTTCATGGTGGACTCAGATGTGAATGCAGTGACCACGGTGC
  ATTCTTCATCAGATCCTTTTGGGAAGACAGATGAGCGGGAACCACTCACAAATGCTGTTCGAAGTGATTC
  GGCAGTCATCGGAGGGGTGATAGCAGTGGTGATATTCATCATCTTCTGTATCATCGGCATCATGACCCGG
  TTCCTCTACCAGCACAAGCAGTCACATCGTACGAGCCAGATGAAGGAGAAGGAATATCCAGAAAATTTGG
  ACAGTTCCTTCAGAAATGAAATTGACTTGCAAAACACAGTGAGCGAGTGTAAACGGGAATATTTCATC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221472 representing NM_130773
  Red=Cloning site Green=Tags(s)
  
  MDSLPRLTSVLTLLFSGLWHLGLTATNYNCDDPLASLLSPMAFSSSSDLTGTHSPAQLNWRVGTGGWSPA
  DSNAQQWLQMDLGNRVEITAVATQGRYGSSDWVTSYSLMFSDTGRNWKQYKQEDSIWTFAGNMNADSVVH
  HKLLHSVRARFVRFVPLEWNPSGKIGMRVEVYGCSYKSDVADFDGRSSLLYRFNQKLMSTLKDVISLKFK
  SMQGDGVLFHGEGQRGDHITLELQKGRLALHLNLGDSKARLSSSLPSATLGSLLDDQHWHSVLIERVGKQ
  VNFTVDKHTQHFRTKGETDALDIDYELSFGGIPVPGKPGTFLKKNFHGCIENLYYNGVNIIDLAKRRKHQ
  IYTGNVTFSCSEPQIVPITFVNSSGSYLLLPGTPQIDGLSVSFQFRTWNKDGLLLSTELSEGSGTLLLSL
  EGGILRLVIQKMTERVAEILTGSNLNDGLWHSVSINARRNRITLTLDDEAAPPAPDSTWVQIYSGNSYYF
  GGCPDNLTDSQCLNPIKAFQGCMRLIFIDNQPKDLISVQQGSLGNFSDLHIDLCSIKDRCLPNYCEHGGS
  CSQSWTTFYCNCSDTSYTGATCHNSIYEQSCEVYRHQGNTAGFFYIDSDGSGPLGPLQVYCNITEDKIWT
  SVQHNNTELTRVRGANPEKPYAMALDYGGSMEQLEAVIDGSEHCEQEVAYHCRRSRLLNTPDGTPFTWWI
  GRSNERHPYWGGSPPGVQQCECGLDESCLDIQHFCNCDADKDEWTNDTGFLSFKDHLPVTQIVITDTDRS
  NSEAAWRIGPLRCYGDRRFWNAVSFYTEASYLHFPTFHAEFSADISFFFKTTALSGVFLENLGIKDFIRL
  EISSPSEITFAIDVGNGPVELVVQSPSLLNDNQWHYVRAERNLKETSLQVDNLPRSTRETSEEGHFRLQL
  NSQLFVGGTSSRQKGFLGCIRSLHLNGQKMDLEERAKVTSGVRPGCPGHCSSYGSICHNGGKCVEKHNGY
  LCDCTNSPYEGPFCKKEVSAVFEAGTSVTYMFQEPYPVTKNISLSSSAIYTDSAPSKENIALSFVTTQAP
  SLLLFINSSSQDFVVVLLCKNGSLQVRYHLNKEETHVFTIDADNFANRRMHHLKINREGRELTIQMDQQL
  RLSYNFSPEVEFRVIRSLTLGKVTENLGLDSEVAKANAMGFAGCMSSVQYNHIAPLKAALRHATVAPVTV
  HGTLTESSCGFMVDSDVNAVTTVHSSSDPFGKTDEREPLTNAVRSDSAVIGGVIAVVIFIIFCIIGIMTR
  FLYQHKQSHRTSQMKEKEYPENLDSSFRNEIDLQNTVSECKREYFI
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130773
• ORF Size: 3918 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130773.1, NM_130773.2, NM_130773.3, NP_570129.1
• RefSeq Size: 5284
• RefSeq ORF: 3921
• Locus ID: 129684
• Domains: F5_F8_type_C, LamG, EGF, EGF
• Protein Families: Transmembrane
• MW: 145.4 kDa
• Gene Summary: This gene product belongs to the neurexin family, members of which function in the vertebrate nervous system as cell adhesion molecules and receptors. This protein, like other neurexin proteins, contains epidermal growth factor repeats and laminin G domains. In addition, it includes an F5/8 type C domain, discoidin/neuropilin- and fibrinogen-like domains, and thrombospondin N-terminal-like domains. [provided by RefSeq, Jul 2008]