C1orf130 (NCMAP) (NM_001010980) Human Tagged ORF Clone

CAT#: RC221481

NCMAP (Myc-DDK-tagged)-Human chromosome 1 open reading frame 130 (C1orf130)

Product Images

Western blot validation of overexpression lysate (Cat# LY423253) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221481 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NCMAP
• Synonyms: C1orf130; MP11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221481 representing NM_001010980
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACAGCCACCCCTCTGGGGGATACCACCTTCTTCTCACTGAACATGACCACCAGGGGAGAAGACT
  TCCTGTATAAGAGTTCTGGAGCCATTGTTGCTGCCGTTGTGGTGGTTGTCATCATCATCTTCACCGTGGT
  TCTGATCCTGCTGAAGATGTACAACAGGAAAATGAGGACGAGGCGGGAACTAGAGCCCAAGGGCCCCAAG
  CCAACCGCCCCTTCTGCCGTGGGCCCAAACAGCAACGGCAGCCAACACCCAGCAACTGTGACCTTCAGTC
  CTGTTGACGTCCAGGTGGAGACGCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221481 representing NM_001010980
  Red=Cloning site Green=Tags(s)
  
  MTTATPLGDTTFFSLNMTTRGEDFLYKSSGAIVAAVVVVVIIIFTVVLILLKMYNRKMRTRRELEPKGPK
  PTAPSAVGPNSNGSQHPATVTFSPVDVQVETR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001010980
• ORF Size: 306 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001010980.1, NM_001010980.2, NM_001010980.3, NM_001010980.4, NP_001010980.1
• RefSeq Size: 3586
• RefSeq ORF: 309
• Locus ID: 400746
• Protein Families: Transmembrane
• MW: 10.9 kDa