LUC7L (NM_201412) Human Tagged ORF Clone

CAT#: RC221529

LUC7L (Myc-DDK-tagged)-Human LUC7-like (S. cerevisiae) (LUC7L), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY403707) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221529 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LUC7L protein (Cat# TP321529). The protein was produced from HEK293T cells transfected with LUC7L cDNA clone (Cat# RC221529) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LUC7L
• Synonyms: hLuc7B1; Luc7; LUC7B1; SR+89
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221529 representing NM_201412
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCCAGGCGCAGATGCGGGCCCTGCTGGACCAGCTCATGGGCACGGCTCGGGACGGAGACGAAA
  CCAGACAGAGGGTCAAGTTTACAGATGACCGTGTCTGCAAGAGTCACCTTCTGGACTGCTGCCCCCATGA
  CATCCTGGCTGGGACGCGCATGGATTTAGGAGAATGTACCAAAATCCACGACTTGGCCCTCCGAGCAGAT
  TATGAGATTGCAAGTAAAGAAAGAGACCTGTTTTTTGAATTAGATGCAATGGATCACTTGGAGTCCTTTA
  TTGCTGAATGTGATCGGAGAACTGAGCTCGCCAAGAAGCGGCTGGCAGAAACACAGGAGGAAATCAGTGC
  GGAAGTTTCTGCAAAGGCAGAAAAAGTACATGAGTTAAATGAAGAAATAGGAAAACTCCTTGCTAAAGCC
  GAACAGCTAGGGGCTGAAGGTAATGTGGATGAATCCCAGAAGATTCTTATGGAAGTGGAAAAAGTTCGTG
  CGAAGAAAAAAGAAGCTGAGGAAGAATACAGAAATTCCATGCCTGCATCCAGTTTTCAGCAGCAAAAGCT
  GCGTGTCTGCGAGGTCTGTTCAGCCTACCTTGGTCTCCATGACAATGACCGTCGCCTGGCAGACCACTTC
  GGTGGCAAGTTACACTTGGGGTTCATTCAGATCCGAGAGAAGCTTGATCAGTTGAGGAAAACTGTCGCTG
  AAAAGCAGGAGAAGAGAAATCAGGATCGCTTGAGGAGGAGAGAGGAGAGGGAACGGGAGGAGCGTCTGAG
  CAGGAGGTCGGGATCAAGAACCAGAGATCGCAGGAGGTCACGCTCCCGGGATCGGCGTCGGAGGCGGTCA
  AGATCTACCTCCCGAGAGCGACGGAAATTGTCCCGGTCCCGGTCCCGAGATAGACATCGGCGCCACCGCA
  GCCGTTCCCGGAGCCACAGCCGGGGACATCGTCGGGCTTCCCGGGACCGAAGTGCGAAATACAAGTTCTC
  CAGAGAGCGGGCATCCAGAGAGGAGTCCTGGGAGAGCGGGCGGAGCGAGCGAGGGCCCCCGGACTGGAGG
  CTTGAGAGCTCCAACGGGAAGATGGCTTCACGGAGGTCAGAAGAGAAGGAGGCCGGCGAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221529 representing NM_201412
  Red=Cloning site Green=Tags(s)
  
  MSAQAQMRALLDQLMGTARDGDETRQRVKFTDDRVCKSHLLDCCPHDILAGTRMDLGECTKIHDLALRAD
  YEIASKERDLFFELDAMDHLESFIAECDRRTELAKKRLAETQEEISAEVSAKAEKVHELNEEIGKLLAKA
  EQLGAEGNVDESQKILMEVEKVRAKKKEAEEEYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHF
  GGKLHLGFIQIREKLDQLRKTVAEKQEKRNQDRLRRREEREREERLSRRSGSRTRDRRRSRSRDRRRRRS
  RSTSRERRKLSRSRSRDRHRRHRSRSRSHSRGHRRASRDRSAKYKFSRERASREESWESGRSERGPPDWR
  LESSNGKMASRRSEEKEAGEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201412
• ORF Size: 1113 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201412.1, NM_201412.2, NP_958815.1
• RefSeq Size: 1452 bp
• RefSeq ORF: 1116 bp
• Locus ID: 55692
• MW: 43.5 kDa
• Gene Summary: The LUC7L gene may represent a mammalian heterochromatic gene, encoding a putative RNA-binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex that is normally required for 5-prime splice site selection (Tufarelli et al., 2001 [PubMed 11170747]). [supplied by OMIM, Mar 2008]