CTRP2 (C1QTNF2) (NM_031908) Human Tagged ORF Clone

CAT#: RC221551

C1QTNF2 (Myc-DDK-tagged)-Human C1q and tumor necrosis factor related protein 2 (C1QTNF2)

Product Images

Western blot validation of overexpression lysate (Cat# LY403129) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221551 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1QTNF2
• Synonyms: CTRP2; zacrp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221551 representing NM_031908
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAACTATGCCTGGGGCCGACGCTCTGCCCGGCTGCTGCCGCTGAGGAAAGCCGGGACGCGGAGC
  CCCGCCGAGAGCTTCTTTGCTCCGGACGCCCCTGGACGTGGCGGGCAGCCGCGAGGGTAACCACCATGAT
  CCCCTGGGTGCTCCTGGCCTGTGCCCTCCCCTGTGCTGCTGACCCACTGCTTGGCGCCTTTGCTCGCAGG
  GACTTCCGGAAAGGCTCCCCTCAACTGGTCTGCAGCCTGCCTGGCCCCCAGGGCCCACCCGGCCCCCCAG
  GAGCCCCAGGGCCCTCAGGAATGATGGGACGAATGGGCTTTCCTGGCAAAGACGGCCAAGATGGACACGA
  CGGCGACCGGGGGGACAGCGGAGAGGAAGGTCCACCTGGCCGGACAGGTAACCGGGGAAAGCCAGGACCA
  AAGGGCAAAGCCGGGGCCATTGGGCGGGCTGGCCCCCGTGGCCCCAAGGGGGTCAACGGTACCCCCGGGA
  AGCATGGCACACCAGGCAAGAAGGGGCCCAAGGGCAAGAAAGGGGAGCCAGGCCTCCCAGGCCCCTGCAG
  CTGTGGCAGTGGCCATACCAAGTCAGCTTTCTCGGTGGCAGTGACCAAGAGCTACCCACGGGAGCGGCTG
  CCCATCAAGTTTGACAAGATTCTGATGAACGAGGGTGGCCACTACAATGCTTCCAGCGGCAAGTTCGTCT
  GCGGCGTGCCTGGGATCTACTACTTCACCTACGACATCACGCTGGCCAACAAGCACCTGGCCATCGGCCT
  GGTGCACAACGGCCAGTACCGCATCCGGACCTTTGATGCCAACACCGGCAACCACGATGTGGCCTCAGGC
  TCCACCATCCTGGCTCTCAAGCAGGGTGACGAAGTTTGGCTGCAGATCTTCTACTCAGAGCAGAACGGGC
  TCTTCTATGACCCTTACTGGACAGACAGCCTCTTTACGGGCTTCCTAATCTATGCCGACCAGGATGACCC
  CAACGAGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221551 representing NM_031908
  Red=Cloning site Green=Tags(s)
  
  MGKLCLGPTLCPAAAAEESRDAEPRRELLCSGRPWTWRAAARVTTMIPWVLLACALPCAADPLLGAFARR
  DFRKGSPQLVCSLPGPQGPPGPPGAPGPSGMMGRMGFPGKDGQDGHDGDRGDSGEEGPPGRTGNRGKPGP
  KGKAGAIGRAGPRGPKGVNGTPGKHGTPGKKGPKGKKGEPGLPGPCSCGSGHTKSAFSVAVTKSYPRERL
  PIKFDKILMNEGGHYNASSGKFVCGVPGIYYFTYDITLANKHLAIGLVHNGQYRIRTFDANTGNHDVASG
  STILALKQGDEVWLQIFYSEQNGLFYDPYWTDSLFTGFLIYADQDDPNEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031908
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031908.4, NP_114114.2
• RefSeq Size: 2411 bp
• RefSeq ORF: 858 bp
• Locus ID: 114898
• Protein Families: Secreted Protein
• MW: 34.7 kDa