C2CD2 (NM_015500) Human Tagged ORF Clone

CAT#: RC221577

C2CD2 (Myc-DDK-tagged)-Human C2 calcium-dependent domain containing 2 (C2CD2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414514) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221577 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C2CD2
• Synonyms: C21orf25; C21orf258; TMEM24L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221577 representing NM_015500
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGGCCCGGCTGGGCTCGTGGCTCGGGGAGGCGCAGTGGCTCGCGCTGGTGTCGCTCTTCGTCG
  CGGCCCTGGCCACGGTAGGCCTGTACCTGGCGCAGTGGGCGCTGGCCAGGGCGCGACCCCAGCCCCAGCG
  GCGGGCGGTGGAGCCTGGAGAGGGGCCGCGCCCGGGGTCCGACGCGCTGCTCTCCTGGATCCTGACGCTG
  GGCAGCTGGAGGAGCCAGTGGCAGGCGGCCTGGGTGACCGCCCTGAACGAGGAGGCCGAGAGGAAAGGGG
  GCCCACCTTTCCTGTCCTTTGAGGAGGACCCGCGGCAGCAGGCACTGGAGCTGGTGGTGCAGGAGGTCTC
  CAGCGTGCTCAGGTCGGCGGAGGAGAAGGTGGTGGTCTGTCACGTGGTGGGCCAGGCTATTCAGTTCTTG
  GTCAGCGAGACGCCTGCCTTGGGTGCTGGATGCCGGCTGTACGACATGCGGCTCTCCCCTTTCCATTTAC
  AGCTGGAGTTCCACATGAAGGAGAAGAGAGAGGACCTCCAGATTAGCTGGTCTTTCATCAGTGTGCCGGA
  AATGGCCGTTAATATCCAGCCCAAAGCACTGGGGGAGGACCAGGTGGCTGAGACAAGTGCGATGTCTGAC
  GTTCTCAAGGACATCTTGAAGCATTTGGCTGGTTCTGCCTCTCCATCAGTGGTTCTGATTACCAAGCCCA
  CGACTGTAAAGGAAGCTCAGAACTTACAGTGTGCTGCATCTACTGCTCAGGAATCCTGTCCTCCTAAACC
  TCCAAGGGCTCACGAGCTGAAGCTACTGGTGAGGAACATCCACGTCTTGCTGCTCAGCGAGCCCGGTGCC
  TCAGGCCACATTAATGCAGTGTGCGTCGTGCAGCTGAACGATCCTGTTCAGAGGTTCTCCAGCACCCTGA
  CGAAAAACACTCCGGACCTCATGTGGGAAGAGGAATTCACCTTCGAGTTGAATGCCAAGTCGAAGGAGTT
  ACACCTGCAGATTTCAGAGGCTGGGCGATCCTCAGAAGGTCTGCTGGCGACGGCGACAGTTCCTCTGGAC
  TTATTTAAGAAGCAGCCTTCTGGGCCACAGAGCTTCACGCTGACCAGCGGGTCTGCCTGCGGCAGCTCAG
  TGCTGGGCTCGGTCACGGCAGAGTTCTCTTACATGGAACCTGGTGAATTGAAATCCTGGCCCATCCCTCC
  CCCTGTTCCTGCTGCAAAAATAGAAAAGGACCGCACGGTGATGCCCTGTGGGACTGTGGTCACTACTGTC
  ACTGCTGTGAAGACCAAGCCTCGCGTCGACGTGGGGAGGGCGTCCCCGCTGAGCTCTGATTCTCCGGTGA
  AGACTCCCATCAAGGTGAAGGTGATCGAGAAGGACATCTCTGTCCAGGCCATCGCCTGCCGCAGCGCCCC
  CGTCAGCAAAACACTCTCTTCTTCAGACACAGAATTGTTGGTGTTGAATGGTTCGGATCCAGTGGCTGAA
  GTGGCCATTCGACAGCTCAGTGAATCTTCAAAGCTGAAACTCAAGTCGCCACGGAAGAAAAGCACTATTA
  TCATATCAGGGATCTCCAAGACCTCACTATCTCAGGACCACGACGCTGCCCTGATGCAGGGCTACACGGC
  CTCTGTGGACAGCACCCACCAGGAGGACGCCCCATCCCATCCGGAGAGGGCGGCAGCCTCTGCCCCGCCA
  GAGGAAGCCGAGTCAGCCCAGGCATCCCTTGCCCCCAAGCCCCAGGAGGACGAGCTAGACTCCTGGGACT
  TGGAGAAGGAGCCACAGGCCGCGGCATGGAGCAGCCAGGTCCTGCTGGACCCCGACGGTGATGAGCTGTC
  AGAGAGCTCCATGAGTGTCTTGGAGCCGGGCACTGCCAAAAAGCATAAAGGAGGAATTCTAAGGAAAGGT
  GCAAAGCTGTTCTTCCGCCGGCGGCATCAACAGAAAGACCCAGGCATGAGTCAGTCACACAATGACCTTG
  TGTTCCTGGAGCAGCCAGAGGGTTCCCGGAGGAAAGGCATCACCCTCACCAGGATCCTGAACAAGAAGCT
  GCTCTCCAGGCACAGAAACAAGAACACCATGAACGGTGCCCCCGTGGAGCCCTGCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221577 representing NM_015500
  Red=Cloning site Green=Tags(s)
  
  MAMARLGSWLGEAQWLALVSLFVAALATVGLYLAQWALARARPQPQRRAVEPGEGPRPGSDALLSWILTL
  GSWRSQWQAAWVTALNEEAERKGGPPFLSFEEDPRQQALELVVQEVSSVLRSAEEKVVVCHVVGQAIQFL
  VSETPALGAGCRLYDMRLSPFHLQLEFHMKEKREDLQISWSFISVPEMAVNIQPKALGEDQVAETSAMSD
  VLKDILKHLAGSASPSVVLITKPTTVKEAQNLQCAASTAQESCPPKPPRAHELKLLVRNIHVLLLSEPGA
  SGHINAVCVVQLNDPVQRFSSTLTKNTPDLMWEEEFTFELNAKSKELHLQISEAGRSSEGLLATATVPLD
  LFKKQPSGPQSFTLTSGSACGSSVLGSVTAEFSYMEPGELKSWPIPPPVPAAKIEKDRTVMPCGTVVTTV
  TAVKTKPRVDVGRASPLSSDSPVKTPIKVKVIEKDISVQAIACRSAPVSKTLSSSDTELLVLNGSDPVAE
  VAIRQLSESSKLKLKSPRKKSTIIISGISKTSLSQDHDAALMQGYTASVDSTHQEDAPSHPERAAASAPP
  EEAESAQASLAPKPQEDELDSWDLEKEPQAAAWSSQVLLDPDGDELSESSMSVLEPGTAKKHKGGILRKG
  AKLFFRRRHQQKDPGMSQSHNDLVFLEQPEGSRRKGITLTRILNKKLLSRHRNKNTMNGAPVEPCT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015500
• ORF Size: 2088 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015500.1, NP_056315.1
• RefSeq Size: 6349 bp
• RefSeq ORF: 2091 bp
• Locus ID: 25966
• Protein Families: Transmembrane
• MW: 75.4 kDa