AK7 (NM_152327) Human Tagged ORF Clone

CAT#: RC221584

AK7 (Myc-DDK-tagged)-Human adenylate kinase 7 (AK7)

Product Images

Western blot validation of overexpression lysate (Cat# LY407632) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221584 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AK7
• Synonyms: AK 7; SPGF27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221584 representing NM_152327
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGAAGAGGAAACTGCTGCTCTCACGGAGAAGGTTATCCGGACCCAGAGGGTGTTTATAAACC
  TGTTGGATTCCTACAGCAGCGGAAACATCGGGAAGTTTCTATCTAACTGTGTAGTTGGGGCTTCGCTTGA
  AGAAATTACAGAGGAAGAGGAAGAGGAAGATGAAAATAAGTCAGCTATGCTGGAAGCTTCCTCAACCAAA
  GTGAAGGAAGGCACATTCCAGATTGTGGGCACGCTGTCCAAGCCTGACAGCCCGCGGCCTGACTTTGCGG
  TGGAGACGTACTCTGCCATCTCTCGAGAAGACCTTCTCATGCGCCTGCTGGAGTGTGATGTTATTATTTA
  TAACATCACTGAGAGCTCACAGCAAATGGAGGAAGCCATCTGGGCAGTCTCTGCACTCAGTGAAGAAGTC
  AGCCACTTTGAAAAGCGAAAGCTATTTATTTTACTGTCGACGGTGATGACTTGGGCGCGCTCCAAAGCCC
  TGGACCCCGAGGATTCTGAGGTTCCATTCACTGAAGAAGATTATCGAAGAAGAAAGTCTCATCCTAATTT
  TCTGGACCACATAAATGCTGAAAAAATGGTTCTCAAATTTGGAAAAAAGGCCAGAAAATTTGCAGCATAC
  GTAGTTGCTGCTGGACTCCAGTATGGAGCGGAAGGAGGCATGTTACACACATTTTTTAAGATGGCTTGGT
  TGGGCGAGATTCCTGCATTACCAGTTTTTGGCGATGGAACAAATGTAATTCCAACAATCCATGTTCTTGA
  TCTAGCAGGAGTGATACAAAACGTCATAGATCACGTGCCAAAGCCTCACTACCTGGTTGCTGTGGATGAG
  TCTGTTCATACCCTGGAAGACATAGTCAAGTGTATCAGTAAAAATACTGGCCCTGGGAAAATCCAGAAAA
  TACCCAGAGAAAATGCATACCTAACCAAGGACTTAACGCAAGATTGTCTTGACCATTTACTGGTCAACTT
  AAGAATGGAAGCGCTCTTTGTGAAGGAGAATTTTAATATTCGATGGGCTGCCCAAACAGGATTTGTGGAA
  AATATCAACACTATCCTCAAGGAGTACAAGCAAAGCAGAGGATTGATGCCAATCAAGATCTGCATTCTTG
  GTCCCCCTGCTGTGGGAAAATCCAGTATTGCTAAAGAATTGGCCAACTACTACAAACTGCATCACATCCA
  ACTGAAGGATGTCATTTCTGAAGCCATAGCAAAACTGGAGGCGATTGTTGCCCCTAACGATGTAGGGGAA
  GGAGAAGAAGAAGTCGAAGAGGAAGAGGAGGAGGAGAATGTGGAAGATGCACAGGAGCTCCTAGATGGCA
  TCAAGGAGAGCATGGAGCAGAATGCAGGTCAACTAGACGATCAATATATAATTAGATTTATGAAAGAAAA
  GCTAAAATCAATGCCTTGCAGGAATCAAGGTTATATTTTGGATGGATTCCCAAAGACCTATGATCAAGCA
  AAAGACCTGTTCAATCAGGAAGATGAGGAGGAGGAAGATGATGTCAGAGGCAGAATGTTTCCCTTTGATA
  AATTAATTATACCTGAATTCGTTTGTGCACTGGATGCTTCGGATGAGTTTCTGAAGGAGCGTGTGATAAA
  CCTTCCTGAGAGCATCGTGGCGGGGACCCACTACAGCCAAGACCGATTCCTCCGGGCTCTGAGCAACTAC
  CGGGACATCAATATCGACGATGAGACTGTCTTCAACTATTTTGATGAACTTGAAATTCACCCGATACATA
  TTGATGTAGGAAAACTTGAAGATGCTCAGAATAGACTTGCTATCAAACAGCTCATCAAAGAGATTGGGGA
  GCCTCGAAATTATGGTTTAACAGACGAAGAAAAGGCAGAAGAGGAGCGGAAGGCTGCGGAGGAGCGGCTG
  GCCAGGGAGGCTGCTGAGGAAGCAGAACGCGAGCACCAGGAGGCCGTGGAGATGGCAGAGAAGATAGCTC
  GCTGGGAGGAGTGGAATAAACGACTGGAGGAAGTGAAAAGAGAAGAAAGAGAATTACTGGAGGCTCAGTC
  AATTCCCCTGAGAAACTATTTAATGACCTATGTGATGCCAACTCTTATTCAGGGCCTGAATGAATGTTGC
  AACGTCCGACCCGAAGACCCTGTTGATTTTCTGGCAGAATATCTCTTCAAGAACAATCCTGAAGCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221584 representing NM_152327
  Red=Cloning site Green=Tags(s)
  
  MAEEEETAALTEKVIRTQRVFINLLDSYSSGNIGKFLSNCVVGASLEEITEEEEEEDENKSAMLEASSTK
  VKEGTFQIVGTLSKPDSPRPDFAVETYSAISREDLLMRLLECDVIIYNITESSQQMEEAIWAVSALSEEV
  SHFEKRKLFILLSTVMTWARSKALDPEDSEVPFTEEDYRRRKSHPNFLDHINAEKMVLKFGKKARKFAAY
  VVAAGLQYGAEGGMLHTFFKMAWLGEIPALPVFGDGTNVIPTIHVLDLAGVIQNVIDHVPKPHYLVAVDE
  SVHTLEDIVKCISKNTGPGKIQKIPRENAYLTKDLTQDCLDHLLVNLRMEALFVKENFNIRWAAQTGFVE
  NINTILKEYKQSRGLMPIKICILGPPAVGKSSIAKELANYYKLHHIQLKDVISEAIAKLEAIVAPNDVGE
  GEEEVEEEEEEENVEDAQELLDGIKESMEQNAGQLDDQYIIRFMKEKLKSMPCRNQGYILDGFPKTYDQA
  KDLFNQEDEEEEDDVRGRMFPFDKLIIPEFVCALDASDEFLKERVINLPESIVAGTHYSQDRFLRALSNY
  RDINIDDETVFNYFDELEIHPIHIDVGKLEDAQNRLAIKQLIKEIGEPRNYGLTDEEKAEEERKAAEERL
  AREAAEEAEREHQEAVEMAEKIARWEEWNKRLEEVKREERELLEAQSIPLRNYLMTYVMPTLIQGLNECC
  NVRPEDPVDFLAEYLFKNNPEAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152327
• ORF Size: 2169 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152327.1, NM_152327.2, NM_152327.3, NP_689540.1, NP_689540.2
• RefSeq Size: 3318
• RefSeq ORF: 2172
• Locus ID: 122481
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism
• MW: 82.7 kDa
• Gene Summary: This gene encodes a member of the adenylate kinase family of enzymes. The encoded enzyme is a phosphotransferase that catalyzes the reversible phosphorylation of adenine nucleotides. This enzyme plays a role in energy homeostasis of the cell. Alternative splicing results in multiple transcript variants. Mutations in the mouse gene are associated with primary ciliary dyskinesia. [provided by RefSeq, Apr 2017]