MYT1L (NM_015025) Human Tagged ORF Clone

CAT#: RC221586

MYT1L (Myc-DDK-tagged)-Human myelin transcription factor 1-like (MYT1L)

Product Images

Western blot validation of overexpression lysate (Cat# LY414829) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221586 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MYT1L
• Synonyms: MRD39; myT1-L; NZF1; ZC2H2C2; ZC2HC4B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221586 representing NM_015025
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGGACACCGAGGAGAAGCGGCATCGCACGCGGTCCAAAGGGGTTCGAGTTCCCGTGGAACCAG
  CCATACAAGAGCTGTTCAGCTGTCCCACCCCTGGCTGTGACGGCAGTGGTCATGTCAGTGGCAAATATGC
  AAGACACAGAAGTGTATATGGTTGTCCCTTGGCGAAAAAAAGAAAAACACAAGATAAACAGCCCCAGGAA
  CCTGCTCCTAAACGAAAGCCATTTGCCGTGAAAGCAGACAGCTCCTCAGTGGATGAGTGTGACGACAGTG
  ATGGGACTGAGGACATGGATGAGAAGGAGGAGGATGAGGGGGAGGAGTACTCCGAGGACAATGACGAGCC
  AGGGGATGAGGACGAGGAGGACGAGGAGGGGGACCGGGAGGAGGAGGAGGAGATCGAGGAGGAGGATGAG
  GACGATGACGAGGATGGAGAAGATGTGGAGGATGAAGAAGAGGAAGAGGAGGAGGAGGAGGAGGAGGAAG
  AGGAAGAAGAAAACGAAGACCATCAAATGAATTGTCACAATACTCGAATAATGCAAGACACAGAAAAGGA
  TGATAACAATAATGACGAATATGACAATTACGATGAACTGGTGGCCAAGTCATTGTTAAACCTCGGCAAA
  ATCGCTGAGGATGCAGCCTACCGGGCCAGGACTGAGTCAGAAATGAACAGCAATACCTCCAATAGTCTGG
  AAGACGATAGTGACAAAAACGAAAACCTGGGTCGGAAAAGTGAGTTGAGTTTAGACTTAGACAGTGATGT
  TGTTAGAGAAACAGTGGACTCCCTTAAACTATTAGCCCAAGGACACGGTGTTGTGCTCTCAGAAAACATG
  AATGACAGAAATTATGCAGACAGCATGTCGCAGCAAGACAGTAGAAATATGAATTACGTCATGTTGGGGA
  AGCCCATGAACAATGGACTCATGGAAAAGATGGTGGAGGAGAGCGATGAGGAGGTGTGTCTGAGCAGTCT
  GGAGTGTTTGAGGAATCAGTGCTTCGACCTGGCCAGGAAGCTCAGTGAGACCAACCCGCAGGAGAGGAAT
  CCGCAGCAGAACATGAACATCCGTCAGCATGTCCGGCCAGAAGAGGACTTCCCAGGAAGGACGCCGGACA
  GAAACTACTCGGACATGCTGAACCTCATGCGGCTGGAGGAGCAGTTGAGCCCCCGGTCGAGAGTGTTTGC
  CAGCTGTGCGAAGGAGGATGGGTGTCATGAGCGGGACGACGATACCACCTCTGTGAACTCGGACAGGTCT
  GAAGAGGTGTTCGACATGACCAAGGGGAACCTGACCCTGCTGGAGAAAGCCATCGCTTTGGAAACGGAAA
  GAGCAAAGGCCATGAGGGAGAAGATGGCCATGGAAGCTGGGAGGAGGGACAATATGAGGTCATATGAGGA
  CCAGTCTCCGAGACAACTTCCCGGGGAGGACAGAAAGCCTAAATCCAGTGACAGCCATGTCAAAAAGCCA
  TACTATGATCCCTCAAGAACAGAAAAGAAAGAGAGCAAGTGTCCAACCCCCGGGTGTGATGGAACCGGCC
  ACGTAACTGGGCTGTACCCACATCACCGCAGCCTGTCCGGATGCCCGCACAAAGATAGGGTCCCTCCAGA
  AATCCTTGCCATGCATGAAAGTGTCCTCAAGTGCCCCACTCCGGGCTGCACGGGGCGCGGGCATGTCAAC
  AGCAACAGGAACTCCCACCGAAGCCTCTCCGGATGCCCGATCGCTGCAGCAGAGAAACTGGCCAAGGCAC
  AGGAAAAGCACCAGAGCTGCGACGTGTCCAAGTCCAGCCAGGCCTCGGACCGCGTGCTCAGGCCAATGTG
  CTTTGTGAAGCAGCTGGAGATTCCTCAGTATGGCTACAGAAACAATGTCCCCACAACTACGCCGCGTTCC
  AACCTGGCCAAGGAGCTCGAGAAATATTCCAAGACCTCGTTTGAATACAACAGTTACGACAACCATACTT
  ATGGCAAGCGAGCCATAGCTCCCAAGGTGCAAACCAGGGATATATCCCCCAAAGGATATGATGATGCGAA
  GCGGTACTGCAAGGACCCCAGCCCCAGCAGCAGCAGCACCAGCAGCTACGCGCCCAGCAGCAGCAGCAAC
  CTGAGCTGCGGCGGGGGCAGCAGCGCCAGCAGCACGTGCAGCAAGAGCAGCTTCGACTACACGCACGACA
  TGGAGGCGGCCCACATGGCGGCCACCGCCATCCTCAACCTGTCCACGCGCTGCCGCGAGATGCCGCAGAA
  CCTGAGCACCAAGCCGCAGGACCTGTGCGCCACGCGGAACCCTGACATGGAGGTGGATGAGAACGGGACC
  CTGGACCTCAGCATGAACAAGCAGAGGCCGCGGGACAGCTGCTGCCCCATCCTGACCCCTCTGGAGCCCA
  TGTCCCCCCAGCAGCAGGCAGTGATGAACAACCGGTGTTTCCAGCTGGGCGAGGGCGACTGCTGGGACTT
  GCCCGTAGACTACACCAAAATGAAACCCCGGAGGATAGACGAGGACGAGTCCAAAGACATTACTCCAGAA
  GACTTGGACCCATTCCAGGAGGCTCTAGAAGAAAGACGGTATCCCGGGGAGGTGACCATCCCAAGTCCCA
  AACCCAAGTACCCTCAGTGCAAGGAGAGCAAAAAGGACTTAATAACTCTGTCTGGCTGCCCCCTGGCGGA
  CAAAAGCATTCGAAGTATGCTGGCCACCAGCTCCCAAGAACTCAAGTGCCCCACGCCTGGCTGTGATGGT
  TCTGGACATATCACCGGCAATTATGCTTCTCATCGGAGCCTTTCAGGTTGCCCAAGAGCAAAGAAAAGTG
  GTATCAGGATAGCACAGAGCAAAGAAGATAAAGAAGATCAAGAACCCATCAGGTGTCCGGTCCCCGGGTG
  CGACGGCCAGGGCCACATCACTGGGAAGTACGCGTCCCATCGCAGCGCCTCCGGGTGCCCCTTGGCGGCC
  AAGAGGCAGAAAGACGGGTACCTGAATGGCTCCCAGTTCTCCTGGAAGTCGGTCAAGACGGAAGGCATGT
  CCTGCCCCACGCCAGGATGCGACGGCTCAGGCCACGTCAGCGGCAGCTTCCTCACACACCGCAGCTTGTC
  AGGATGCCCGAGAGCCACGTCAGCGATGAAGAAGGCAAAGCTTTCTGGAGAGCAGATGCTGACCATCAAA
  CAGCGGGCCAGCAACGGTATAGAAAATGATGAAGAAATCAAACAGTTAGATGAAGAAATCAAGGAGCTAA
  ATGAATCCAATTCCCAGATGGAAGCCGATATGATTAAACTCAGAACTCAGATTACCACGATGGAGAGCAA
  CCTGAAGACCATCGAAGAGGAGAACAAAGTGATTGAGCAGCAGAACGAGTCTCTCCTCCACGAGCTGGCG
  AACCTGAGCCAGTCTCTGATCCACAGCCTGGCTAACATCCAGCTGCCGCACATGGATCCAATCAATGAAC
  AAAATTTTGATGCTTACGTGACTACTTTGACGGAAATGTATACAAATCAAGATCGTTATCAGAGTCCAGA
  AAATAAAGCCCTACTGGAAAATATAAAGCAGGCTGTGAGAGGAATTCAGGTC
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221586 representing NM_015025
  Red=Cloning site Green=Tags(s)
  
  MEVDTEEKRHRTRSKGVRVPVEPAIQELFSCPTPGCDGSGHVSGKYARHRSVYGCPLAKKRKTQDKQPQE
  PAPKRKPFAVKADSSSVDECDDSDGTEDMDEKEEDEGEEYSEDNDEPGDEDEEDEEGDREEEEEIEEEDE
  DDDEDGEDVEDEEEEEEEEEEEEEEEENEDHQMNCHNTRIMQDTEKDDNNNDEYDNYDELVAKSLLNLGK
  IAEDAAYRARTESEMNSNTSNSLEDDSDKNENLGRKSELSLDLDSDVVRETVDSLKLLAQGHGVVLSENM
  NDRNYADSMSQQDSRNMNYVMLGKPMNNGLMEKMVEESDEEVCLSSLECLRNQCFDLARKLSETNPQERN
  PQQNMNIRQHVRPEEDFPGRTPDRNYSDMLNLMRLEEQLSPRSRVFASCAKEDGCHERDDDTTSVNSDRS
  EEVFDMTKGNLTLLEKAIALETERAKAMREKMAMEAGRRDNMRSYEDQSPRQLPGEDRKPKSSDSHVKKP
  YYDPSRTEKKESKCPTPGCDGTGHVTGLYPHHRSLSGCPHKDRVPPEILAMHESVLKCPTPGCTGRGHVN
  SNRNSHRSLSGCPIAAAEKLAKAQEKHQSCDVSKSSQASDRVLRPMCFVKQLEIPQYGYRNNVPTTTPRS
  NLAKELEKYSKTSFEYNSYDNHTYGKRAIAPKVQTRDISPKGYDDAKRYCKDPSPSSSSTSSYAPSSSSN
  LSCGGGSSASSTCSKSSFDYTHDMEAAHMAATAILNLSTRCREMPQNLSTKPQDLCATRNPDMEVDENGT
  LDLSMNKQRPRDSCCPILTPLEPMSPQQQAVMNNRCFQLGEGDCWDLPVDYTKMKPRRIDEDESKDITPE
  DLDPFQEALEERRYPGEVTIPSPKPKYPQCKESKKDLITLSGCPLADKSIRSMLATSSQELKCPTPGCDG
  SGHITGNYASHRSLSGCPRAKKSGIRIAQSKEDKEDQEPIRCPVPGCDGQGHITGKYASHRSASGCPLAA
  KRQKDGYLNGSQFSWKSVKTEGMSCPTPGCDGSGHVSGSFLTHRSLSGCPRATSAMKKAKLSGEQMLTIK
  QRASNGIENDEEIKQLDEEIKELNESNSQMEADMIKLRTQITTMESNLKTIEEENKVIEQQNESLLHELA
  NLSQSLIHSLANIQLPHMDPINEQNFDAYVTTLTEMYTNQDRYQSPENKALLENIKQAVRGIQV
  
  TRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015025
• ORF Size: 3552 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015025.2, NM_015025.3, NP_055840.2
• RefSeq Size: 7152
• RefSeq ORF: 3555
• Locus ID: 23040
• Protein Families: Transcription Factors
• MW: 132.7 kDa
• Gene Summary: This gene encodes a member of the zinc finger superfamily of transcription factors whose expression, thus far, has been found only in neuronal tissues. The encoded protein belongs to a novel class of cystein-cystein-histidine-cystein zinc finger proteins that function in the developing mammalian central nervous system. Forced expression of this gene in combination with the basic helix-loop-helix transcription factor NeuroD1 and the transcription factors POU class 3 homeobox 2 and achaete-scute family basic helix-loop-helix transcription factor 1 can convert fetal and postnatal human fibroblasts into induced neuronal cells, which are able to generate action potentials. Mutations in this gene have been associated with an autosomal dominant form of cognitive disability and with autism spectrum disorder. Alternative splicing results in multiple variants. [provided by RefSeq, Jul 2017]