PCDHA7 (NM_018910) Human Tagged ORF Clone

CAT#: RC221616

PCDHA7 (Myc-DDK-tagged)-Human protocadherin alpha 7 (PCDHA7), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412922) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221616 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHA7
• Synonyms: CNR4; CNRN4; CNRS4; CRNR4; PCDH-ALPHA7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221616 representing NM_018910
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTGCCCGAATGGATACGACCCAGGGGGCCGACATCTACTGCTGTTTATTATAATTCTAGCAGCTT
  GGGAGGCAGGGAGAGGCCAGCTCCACTACTCGGTCCCCGAGGAGGCTAAACATGGCAACTTCGTGGGCCG
  CATCGCGCAGGACCTGGGGCTGGAGCTGGCGGAGCTGGTGCCGCGCCTGTTCCGGGCGGTGTGCAAATTC
  CGTGGGGATCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGCGAGG
  AGCTGTGCGGGCGGAGCGCGGAGTGCAGCATCCACCTGGAGGTGATCGTGGAAAGGCCGCTGCAGGTTTT
  CCATGTGGACGTGGAGGTGAAGGACATTAACGACAACCCTCCGGTGTTCCCAGCGACACAAAGGAATCTG
  TTCATCGCGGAATCCAGGCCGCTTGACTCTCGGTTTCCACTAGAGGGCGCGTCCGATGCAGATATCGGGG
  AGAACGCCCTGCTCACTTACAGACTGAGCCCCAATGAGTATTTCTTCCTGGACGTGCCAACCAGCAACCA
  GCAGGTAAAACCTCTTGGACTTGTATTACGGAAACTTTTAGACAGAGAAGAAACTCCGGAGCTTCATTTA
  TTGCTCACGGCCACCGATGGAGGCAAACCCGAGCTGACTGGCACCGTTCAATTACTCATCACGGTACTGG
  ACAACAATGACAATGCCCCAGTGTTCGACAGAACCCTGTATACGGTGAAATTACCAGAAAACGTTTCTAT
  CGGAACGCTGGTGATTCACCCCAATGCCTCAGATTTAGACGAAGGCTTGAATGGGGATATTATTTACTCC
  TTCTCCAGTGATGTTTCTCCAGATATAAAATCCAAGTTCCACATGGACCCCTTAAGTGGGGCAATCACAG
  TGATAGGACATATGGATTTTGAAGAAAGTAGAGCACACAAGATCCCAGTCGAGGCTGTCGATAAAGGCTT
  CCCACCCCTGGCTGGTCATTGTACAGTTCTTGTGGAAGTTGTGGATGTAAATGACAATGCTCCACAGTTG
  ACTCTCACTTCCCTGTCTCTCCCTATTCCAGAGGACGCCCAACCAGGTACCGTCATCACATTGATTAGCG
  TGTTTGACCGAGATTTTGGAGTCAACGGACAGGTTACCTGCTCCCTGACGCCCCGCGTTCCCTTCAAGTT
  GGTGTCCACCTTCAAGAATTACTATTCATTGGTGCTGGACAGCGCTCTGGACCGCGAGAGTGTGTCCGCC
  TATGAGCTGGTGGTTACCGCGCGGGACGGGGGCTCGCCTTCTCTGTGGGCCACTGCTAGCGTGTCCGTGG
  AGGTGGCCGACGTGAACGACAACGCCCCGGCGTTCGCGCAGCCCGAGTATACGGTGTTCGTGAAGGAGAA
  CAACCCGCCGGGCTGCCACATCTTCACTGTGTCGGCGGGGGACGCGGACGCGCAGAAGAACGCGCTGGTG
  TCCTACTCGCTGGTGGAGCTGCGGGTGGGCGAGCGCGCGCTGTCGAGCTACGTGTCAGTGCACGCGGAGA
  GCGGCAAGGTGTACGCGCTGCAGCCGTTGGACCACGAGGAGCTGGAGCTGTTGCAGTTCCAGGTGAGCGC
  GCGCGATGCGGGCGTGCCGCCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAGAACGAC
  AACGCGCCGGCACTGCTGGCGCCTCGGGTGGGTGGCACTGGTGGCGCAGTGAGAGAGCTTGTGCCGCGGT
  CTGTGGGCGCGGGCCATGTGGTGGCGAAGGTACGTGCAGTTGACGCTGACTCAGGCTACAACGCGTGGCT
  TTCGTATGAGTTGCAACCGGTGGCGGCCGGTGCGAGCATCCCGTTCCGCGTGGGGCTGTACACTGGTGAG
  ATCAGCACGACACGAGCCCTAGATGAGACGGACGCACCGCGCCACCGCCTTCTGGTGCTTGTGAAGGACC
  ACGGGGAGCCCTCGCTGACAGCCACAGCCACCGTGCTGGTGTCGCTGGTGGAAAGCGGCCAGGCACCAAA
  GGCGTCGTCGCGGGCATCGTTGGGCATTGCAGGCCCAGAGACCGAGCTGGTGGATGTCAACGTGTACCTG
  ATCATCGCCATCTGCGCGGTGTCCAGTCTGTTGGTGCTTACCCTGCTGCTGTACACGGCGTTGCGGTGCT
  CAGCGCCGTCCTCTGAGGGCGCATGTAGTTTGGTAAAGCCCACTCTGGTGTGCTCCAGCGCGGTGGGGAG
  CTGGTCATTCTCCCAGCAGAGGCGGCAGAGGGTGTGCTCTGGGGAGGGCCCACCCAAGACAGACCTCATG
  GCCTTCAGTCCCAGCCTTCCTCAGGGTCCATCCTCTACAGACAATCCACGACAGCCCAACCCTGACTGGC
  GTTACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGTGCACCTAGAGGAGGCTGGCATTCTACGGGC
  TGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAGAACCAGAGGCAGGAGAA
  GTGTCCCCTCCAGTCGGTGCGGGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCA
  AACAATCCGGTCCCGGTGAGTTGCCCGACAAATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCG
  GCAGGAGCCTACTAACAGCCAAATTGACAAAAGTGACTTCATAACCTTCGGCAAAAAGGAGGAGACCAAG
  AAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAAGGGAACAGCACGACTGACA
  ACAGTGACCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221616 representing NM_018910
  Red=Cloning site Green=Tags(s)
  
  MVCPNGYDPGGRHLLLFIIILAAWEAGRGQLHYSVPEEAKHGNFVGRIAQDLGLELAELVPRLFRAVCKF
  RGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVERPLQVFHVDVEVKDINDNPPVFPATQRNL
  FIAESRPLDSRFPLEGASDADIGENALLTYRLSPNEYFFLDVPTSNQQVKPLGLVLRKLLDREETPELHL
  LLTATDGGKPELTGTVQLLITVLDNNDNAPVFDRTLYTVKLPENVSIGTLVIHPNASDLDEGLNGDIIYS
  FSSDVSPDIKSKFHMDPLSGAITVIGHMDFEESRAHKIPVEAVDKGFPPLAGHCTVLVEVVDVNDNAPQL
  TLTSLSLPIPEDAQPGTVITLISVFDRDFGVNGQVTCSLTPRVPFKLVSTFKNYYSLVLDSALDRESVSA
  YELVVTARDGGSPSLWATASVSVEVADVNDNAPAFAQPEYTVFVKENNPPGCHIFTVSAGDADAQKNALV
  SYSLVELRVGERALSSYVSVHAESGKVYALQPLDHEELELLQFQVSARDAGVPPLGSNVTLQVFVLDEND
  NAPALLAPRVGGTGGAVRELVPRSVGAGHVVAKVRAVDADSGYNAWLSYELQPVAAGASIPFRVGLYTGE
  ISTTRALDETDAPRHRLLVLVKDHGEPSLTATATVLVSLVESGQAPKASSRASLGIAGPETELVDVNVYL
  IIAICAVSSLLVLTLLLYTALRCSAPSSEGACSLVKPTLVCSSAVGSWSFSQQRRQRVCSGEGPPKTDLM
  AFSPSLPQGPSSTDNPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGE
  VSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDKSDFITFGKKEETK
  KKKKKKKGNKTQEKKEKGNSTTDNSDQ
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018910
• ORF Size: 2811 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018910.1, NM_018910.2, NP_061733.1
• RefSeq Size: 5221
• RefSeq ORF: 2814
• Locus ID: 56141
• Domains: CA
• Protein Families: Transmembrane
• MW: 97.7 kDa
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]