LYK5 (STRADA) (NM_001003786) Human Tagged ORF Clone

CAT#: RC221633

STRADA (Myc-DDK-tagged)-Human STE20-related kinase adaptor alpha (STRADA), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STRADA
• Synonyms: LYK5; NY-BR-96; PMSE; Stlk; STRAD; STRAD alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221633 representing NM_001003786
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATTTCTTACCAATGATGCGAGCTCAGAGTCAATAGCATCCTTCTCTAAACAGGAGGTCATGAGTA
  GCTTTCTGCCAGAGGGAGGGTGTTACGAGCTGCTCACTGTGATAGGCAAAGGATTTGAGGACCTGATGAC
  TGTGAATCTAGCAAGGTACAAACCAACAGGAGAGTACGTGACTGTACGGAGGATTAACCTAGAAGCTTGT
  TCCAATGAGATGGTAACATTCTTGCAGGGCGAGCTGCATGTCTCCAAACTCTTCAACCATCCCAATATCG
  TGCCATATCGAGCCACTTTTATTGCAGACAATGAGCTGTGGGTTGTCACATCATTCATGGCATACGGTTC
  TGCAAAAGATCTCATCTGTACACACTTCATGGATGGCATGAATGAGCTGGCGATTGCTTACATCCTGCAG
  GGGGTGCTGAAGGCCCTCGACTACATCCACCACATGGGATATGTACACAGGAGTGTCAAAGCCAGCCACA
  TCCTGATCTCTGTGGATGGGAAGGTCTACCTGTCTGGTTTGCGCAGCAACCTCAGCATGATAAGCCATGG
  GCAGCGGCAGCGAGTGGTCCACGATTTTCCCAAGTACAGTGTCAAGGTTCTGCCGTGGCTCAGCCCCGAG
  GTCCTCCAGCAGAATCTCCAGGGTTATGATGCCAAGTCTGACATCTACAGTGTGGGAATCACAGCCTGTG
  AACTGGCCAACGGCCATGTCCCCTTTAAGGATATGCCTGCCACCCAGATGCTGCTAGAGAAACTGAACGG
  CACAGTGCCCTGCCTGTTGGATACCAGCACCATCCCCGCTGAGGAGCTGACCATGAGCCCTTCGCGCTCA
  GTGGCCAACTCTGGCCTGAGTGACAGCCTGACCACCAGCACCCCCCGGCCCTCCAACGGTGACTCGCCCT
  CCCACCCCTACCACCGAACCTTCTCCCCCCACTTCCACCACTTTGTGGAGCAGTGCCTTCAGCGCAACCC
  GGATGCCAGGCCCAGTGCCAGCACCCTCCTGAACCACTCTTTCTTCAAGCAGATCAAGCGACGTGCCTCA
  GAGGCTTTGCCCGAATTGCTTCGTCCTGTCACCCCCATCACCAATTTTGAGGGCAGCCAGTCTCAGGACC
  ACAGTGGAATCTTTGGCCTGGTAACAAACCTGGAAGAGCTGGAGGTGGACGATTGGGAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221633 representing NM_001003786
  Red=Cloning site Green=Tags(s)
  
  MSFLTNDASSESIASFSKQEVMSSFLPEGGCYELLTVIGKGFEDLMTVNLARYKPTGEYVTVRRINLEAC
  SNEMVTFLQGELHVSKLFNHPNIVPYRATFIADNELWVVTSFMAYGSAKDLICTHFMDGMNELAIAYILQ
  GVLKALDYIHHMGYVHRSVKASHILISVDGKVYLSGLRSNLSMISHGQRQRVVHDFPKYSVKVLPWLSPE
  VLQQNLQGYDAKSDIYSVGITACELANGHVPFKDMPATQMLLEKLNGTVPCLLDTSTIPAEELTMSPSRS
  VANSGLSDSLTTSTPRPSNGDSPSHPYHRTFSPHFHHFVEQCLQRNPDARPSASTLLNHSFFKQIKRRAS
  EALPELLRPVTPITNFEGSQSQDHSGIFGLVTNLEELEVDDWEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003786
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001003786.1, NM_001003786.2, NP_001003786.1
• RefSeq Size: 2112
• RefSeq ORF: 1185
• Locus ID: 92335
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: mTOR signaling pathway
• MW: 43.8 kDa
• Gene Summary: The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq, Sep 2009]