REV1 (NM_016316) Human Tagged ORF Clone

CAT#: RC221742

REV1 (Myc-DDK-tagged)-Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402538) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221742 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: REV1
• Synonyms: AIBP80; REV1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221742 representing NM_016316
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGAGGTGGATGGAGGAAGCGAGCTGAAAATGATGGCTGGGAAACATGGGGTGGGTATATGGCTG
  CCAAGGTCCAGAAATTGGAGGAACAGTTTCGATCAGATGCTGCTATGCAGAAGGATGGGACTTCATCTAC
  AATTTTTAGTGGAGTTGCCATCTATGTTAATGGATACACAGATCCTTCCGCTGAGGAATTGAGAAAACTA
  ATGATGTTGCATGGAGGTCAATACCATGTATATTATTCCAGATCTAAAACAACACATATTATTGCCACAA
  ATCTTCCCAATGCCAAAATTAAAGAATTAAAGGGGGAAAAAGTAATTCGACCAGAATGGATTGTGGAAAG
  CATCAAAGCTGGACGACTCCTCTCCTACATTCCATATCAGCTGTACACCAAGCAGTCCAGTGTGCAGAAA
  GGTCTCAGCTTTAATCCTGTATGCAGACCTGAGGATCCTCTGCCAGGTCCAAGCAATATAGCCAAACAGC
  TCAACAACAGGGTAAATCACATCGTTAAGAAGATTGAAACGGAAAATGAAGTCAAAGTCAATGGCATGAA
  CAGTTGGAATGAAGAAGATGAAAATAATGATTTTAGTTTTGTGGATCTGGAGCAGACCTCTCCGGGAAGG
  AAACAGAATGGAATTCCGCATCCCAGAGGGAGCACTGCCATTTTTAATGGACACACTCCTAGCTCTAATG
  GTGCCTTAAAGACACAGGATTGCTTGGTGCCCATGGTCAACAGTGTTGCCAGCAGGCTTTCTCCAGCCTT
  TTCCCAGGAGGAGGATAAGGCTGAGAAGAGCAGCACTGATTTCAGAGACTGCACTCTGCAGCAGTTGCAG
  CAAAGCACCAGAAACACAGATGCTTTGCGGAATCCACACAGAACTAATTCTTTCTCATTATCACCTTTGC
  ACAGTAACACTAAAATCAATGGTGCTCACCACTCCACTGTTCAGGGGCCTTCAAGCACAAAAAGCACTTC
  TTCAGTATCTACGTTTAGCAAGGCAGCACCTTCAGTGCCATCCAAACCTTCAGACTGCAATTTTATTTCA
  AACTTCTATTCTCATTCAAGACTGCATCACATATCAATGTGGAAGTGTGAATTGACTGAGTTTGTCAATA
  CCCTACAAAGACAAAGTAATGGTATCTTTCCAGGAAGGGAAAAGTTAAAAAAAATGAAAACAGGCAGGTC
  TGCACTTGTTGTAACTGACACAGGAGATATGTCAGTATTGAATTCTCCCAGACATCAGAGCTGTATAATG
  CATGTTGATATGGATTGCTTCTTTGTATCAGTGGGTATACGAAATAGACCAGATCTCAAAGGAAAACCAG
  TGGCTGTTACAAGTAACAGAGGCACAGGAAGGGCACCTTTACGTCCTGGCGCTAACCCCCAGCTGGAGTG
  GCAGTATTACCAGAATAAAATCCTGAAAGGCAAAGCAGCAGATATACCAGATTCATCATTGTGGGAGAAT
  CCAGATTCTGCGCAAGCAAATGGAATTGATTCTGTTTTGTCAAGGGCTGAAATTGCATCTTGTAGTTATG
  AGGCCAGGCAACTTGGCATTAAGAACGGAATGTTTTTTGGGCATGCTAAACAACTATGTCCTAATCTTCA
  AGCTGTTCCATACGATTTTCATGCATATAAGGAAGTCGCACAAACATTGTATGAAACATTGGCAAGCTAC
  ACTCATAACATTGAAGCTGTCAGTTGTGATGAAGCGCTGGTAGACATTACCGAAATCCTTGCAGAGACCA
  AACTTACTCCTGATGAATTTGCAAATGCTGTTCGTATGGAAATCAAAGACCAGACGAAATGTGCTGCCTC
  TGTTGGAATTGGTTCTAATATTCTCCTGGCTAGAATGGCAACTAGAAAAGCAAAACCAGATGGGCAGTAC
  CACCTAAAACCAGAAGAAGTAGATGATTTTATCAGAGGCCAGCTAGTGACCAATCTACCAGGAGTTGGAC
  ATTCAATGGAATCTAAGTTGGCATCTTTGGGAATTAAAACTTGTGGAGACTTGCAGTATATGACCATGGC
  AAAACTCCAAAAAGAATTTGGTCCCAAAACAGGTCAGATGCTTTATAGGTTCTGCCGTGGCTTGGATGAT
  AGACCAGTTCGAACTGAAAAGGAAAGAAAATCTGTTTCAGCTGAGATCAACTATGGAATAAGGTTTACTC
  AGCCAAAAGAGGCAGAAGCTTTTCTTCTGAGTCTTTCAGAAGAAATTCAAAGAAGACTAGAAGCCACTGG
  CATGAAGGGTAAACGTCTAACTCTCAAAATCATGGTACGAAAGCCTGGGGCTCCTGTAGAAACTGCAAAA
  TTTGGAGGCCATGGAATTTGTGATAACATTGCCAGGACTGTAACTCTTGACCAGGCAACAGATAATGCAA
  AAATAATTGGAAAGGCGATGCTAAACATGTTTCATACAATGAAACTAAATATATCAGATATGAGAGGGGT
  TGGGATTCACGTGAATCAGTTGGTTCCAACTAATCTGAACCCTTCCACATGTCCCAGTCGCCCATCAGTT
  CAGTCAAGCCACTTTCCTAGTGGGTCATACTCTGTCCGTGATGTCTTCCAAGTTCAGAAAGCTAAGAAAT
  CCACCGAAGAGGAGCACAAAGAAGTATTTCGGGCTGCTGTGGATCTGGAAATATCATCTGCTTCTAGAAC
  TTGCACTTTCTTGCCACCTTTTCCTGCACATCTGCCGACCAGTCCTGATACTAACAAGGCTGAGTCTTCA
  GGGAAATGGAATGGTCTACATACTCCTGTCAGTGTGCAGTCGAGACTTAACCTGAGTATAGAGGTCCCGT
  CACCTTCCCAGCTGGATCAGTCTGTTTTAGAAGCACTTCCACCTGATCTCCGGGAACAAGTAGAGCAAGT
  CTGTGCTGTCCAGCAAGCAGAGTCACATGGCGACAAAAAGAAAGAACCAGTAAATGGCTGTAATACAGGA
  ATTTTGCCACAACCAGTTGGGACAGTCTTGTTGCAAATACCAGAACCTCAAGAATCGAACAGTGACGCAG
  GAATAAATTTAATAGCCCTTCCAGCATTTTCACAGGTGGACCCTGAGGTATTTGCTGCCCTTCCTGCTGA
  ACTTCAGAGGGAGCTGAAAGCAGCGTATGATCAAAGACAAAGGCAGGGCGAGAACAGCACTCACCAGCAG
  TCAGCCAGCGCATCTGTGCCAAAGAATCCTTTACTTCATCTAAAGGCAGCAGTGAAAGAAAAGAAAAGAA
  ACAAGAAGAAAAAAACCATTGGTTCACCAAAAAGGATTCAGAGTCCTTTGAATAACAAGCTGCTTAACAG
  TCCTGCAAAAACTCTGCCAGGGGCCTGTGGCAGTCCCCAGAAGTTAATTGATGGGTTTCTAAAACATGAA
  GGACCTCCTGCAGAGAAACCCCTGGAAGAACTCTCTGCTTCTACTTCAGGTGTGCCAGGCCTTTCTAGTT
  TGCAGTCTGACCCAGCTGGCTGTGTGAGACCTCCAGCACCCAATCTAGCTGGAGCTGTTGAATTCAATGA
  TGTGAAGACCTTGCTCAGAGAATGGATAACTACAATTTCAGATCCAATGGAAGAAGACATTCTCCAAGTT
  GTGAAATACTGTACTGATCTAATAGAAGAAAAAGATTTGGAAAAACTGGATCTAGTTATAAAATACATGA
  AAAGGCTGATGCAGCAATCGGTGGAATCGGTTTGGAATATGGCATTTGACTTTATTCTTGACAATGTCCA
  GGTGGTTTTACAACAAACTTATGGAAGCACATTAAAAGTTACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221742 representing NM_016316
  Red=Cloning site Green=Tags(s)
  
  MRRGGWRKRAENDGWETWGGYMAAKVQKLEEQFRSDAAMQKDGTSSTIFSGVAIYVNGYTDPSAEELRKL
  MMLHGGQYHVYYSRSKTTHIIATNLPNAKIKELKGEKVIRPEWIVESIKAGRLLSYIPYQLYTKQSSVQK
  GLSFNPVCRPEDPLPGPSNIAKQLNNRVNHIVKKIETENEVKVNGMNSWNEEDENNDFSFVDLEQTSPGR
  KQNGIPHPRGSTAIFNGHTPSSNGALKTQDCLVPMVNSVASRLSPAFSQEEDKAEKSSTDFRDCTLQQLQ
  QSTRNTDALRNPHRTNSFSLSPLHSNTKINGAHHSTVQGPSSTKSTSSVSTFSKAAPSVPSKPSDCNFIS
  NFYSHSRLHHISMWKCELTEFVNTLQRQSNGIFPGREKLKKMKTGRSALVVTDTGDMSVLNSPRHQSCIM
  HVDMDCFFVSVGIRNRPDLKGKPVAVTSNRGTGRAPLRPGANPQLEWQYYQNKILKGKAADIPDSSLWEN
  PDSAQANGIDSVLSRAEIASCSYEARQLGIKNGMFFGHAKQLCPNLQAVPYDFHAYKEVAQTLYETLASY
  THNIEAVSCDEALVDITEILAETKLTPDEFANAVRMEIKDQTKCAASVGIGSNILLARMATRKAKPDGQY
  HLKPEEVDDFIRGQLVTNLPGVGHSMESKLASLGIKTCGDLQYMTMAKLQKEFGPKTGQMLYRFCRGLDD
  RPVRTEKERKSVSAEINYGIRFTQPKEAEAFLLSLSEEIQRRLEATGMKGKRLTLKIMVRKPGAPVETAK
  FGGHGICDNIARTVTLDQATDNAKIIGKAMLNMFHTMKLNISDMRGVGIHVNQLVPTNLNPSTCPSRPSV
  QSSHFPSGSYSVRDVFQVQKAKKSTEEEHKEVFRAAVDLEISSASRTCTFLPPFPAHLPTSPDTNKAESS
  GKWNGLHTPVSVQSRLNLSIEVPSPSQLDQSVLEALPPDLREQVEQVCAVQQAESHGDKKKEPVNGCNTG
  ILPQPVGTVLLQIPEPQESNSDAGINLIALPAFSQVDPEVFAALPAELQRELKAAYDQRQRQGENSTHQQ
  SASASVPKNPLLHLKAAVKEKKRNKKKKTIGSPKRIQSPLNNKLLNSPAKTLPGACGSPQKLIDGFLKHE
  GPPAEKPLEELSASTSGVPGLSSLQSDPAGCVRPPAPNLAGAVEFNDVKTLLREWITTISDPMEEDILQV
  VKYCTDLIEEKDLEKLDLVIKYMKRLMQQSVESVWNMAFDFILDNVQVVLQQTYGSTLKVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016316
• ORF Size: 3753 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016316.1, NM_016316.2, NM_016316.3, NP_057400.1
• RefSeq Size: 4276 bp
• RefSeq ORF: 3756 bp
• Locus ID: 51455
• Domains: IMS, BRCT
• Protein Families: Druggable Genome
• MW: 138.1 kDa
• Gene Summary: This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]