Complement C8A (C8A) (NM_000562) Human Tagged ORF Clone
CAT#: RC221824
C8A (Myc-DDK-tagged)-Human complement component 8, alpha polypeptide (C8A)
"NM_000562" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | C8A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC221824 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGCTGTTGTTTTCTTCATCTTGTCTTTGATGACTTGTCAGCCTGGGGTAACTGCACAGGAGAAGG TGAACCAGAGAGTAAGACGGGCAGCTACACCCGCAGCAGTTACCTGCCAGCTGAGCAACTGGTCAGAGTG GACAGATTGCTTTCCGTGCCAGGACAAAAAGTACCGACACCGGAGCCTCTTGCAGCCAAACAAGTTTGGG GGAACCATCTGCAGTGGTGACATCTGGGATCAAGCCAGCTGCTCCAGTTCTACAACTTGTGTAAGGAAAG CACAGTGTGGACAGGATTTCCAGTGTAAGGAGACAGGTCGCTGCCTGAAACGCCACCTTGTGTGTAATGG AGACCAGGACTGCCTTGATGGCTCTGATGAGGACGACTGTGAAGATGTCAGGGCCATTGACGAAGACTGC AGCCAGTATGAACCAATTCCAGGATCACAGAAGGCAGCCTTGGGGTACAATATCCTGACCCAGGAAGATG CTCAGAGTGTGTACGATGCCAGTTATTATGGGGGCCAGTGTGAGACGGTATACAATGGGGAATGGAGGGA GCTTCGATATGACTCCACCTGTGAACGTCTCTACTATGGAGATGATGAGAAATACTTTCGGAAACCCTAC AACTTTCTGAAGTACCACTTTGAAGCCCTGGCAGATACTGGAATCTCCTCAGAGTTTTATGATAATGCAA ATGACCTTCTTTCCAAAGTTAAAAAAGACAAGTCTGACTCATTTGGAGTGACCATCGGCATAGGCCCAGC CGGCAGCCCTTTATTGGTGGGTGTAGGTGTATCCCACTCACAAGACACTTCATTCTTGAACGAATTAAAC AAGTATAATGAGAAGAAATTCATTTTCACAAGAATCTTCACAAAGGTGCAGACTGCACATTTTAAGATGA GGAAGGATGACATTATGCTGGATGAAGGAATGCTGCAGTCATTAATGGAGCTTCCAGATCAGTACAATTA TGGCATGTATGCCAAGTTCATCAATGACTATGGCACCCATTACATCACATCTGGATCCATGGGTGGCATT TATGAATATATCCTGGTGATTGACAAAGCAAAAATGGAATCCCTTGGTATTACCAGCAGAGATATCACGA CATGTTTTGGAGGCTCCTTGGGCATTCAATATGAAGACAAAATAAATGTTGGTGGAGGTTTATCAGGAGA CCATTGTAAAAAATTTGGAGGTGGCAAAACTGAAAGGGCCAGGAAGGCCATGGCTGTGGAAGACATTATT TCTCGGGTGCGAGGTGGCAGTTCTGGCTGGAGCGGTGGCTTGGCACAGAACAGGAGCACCATTACATACC GTTCCTGGGGGAGGTCATTAAAGTATAATCCTGTTGTTATCGATTTTGAGATGCAGCCTATCCACGAGGT GCTGCGGCACACAAGCCTGGGGCCTCTGGAGGCCAAGCGCCAGAACCTGCGCCGCGCCTTGGACCAGTAT CTGATGGAATTCAATGCCTGCCGATGTGGGCCTTGCTTCAACAATGGGGTGCCCATCCTCGAGGGCACCA GCTGCAGGTGCCAGTGCCGCCTGGGTAGCTTGGGTGCTGCCTGTGAGCAAACACAGACAGAAGGAGCCAA AGCAGATGGGAGCTGGAGTTGCTGGAGCTCCTGGTCTGTATGCAGAGCAGGCATCCAGGAAAGGAGAAGA GAGTGTGACAATCCAGCACCTCAGAATGGAGGGGCCTCGTGTCCAGGGCGGAAAGTACAGACGCAGGCTT GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC221824 protein sequence
Red=Cloning site Green=Tags(s) MFAVVFFILSLMTCQPGVTAQEKVNQRVRRAATPAAVTCQLSNWSEWTDCFPCQDKKYRHRSLLQPNKFG GTICSGDIWDQASCSSSTTCVRKAQCGQDFQCKETGRCLKRHLVCNGDQDCLDGSDEDDCEDVRAIDEDC SQYEPIPGSQKAALGYNILTQEDAQSVYDASYYGGQCETVYNGEWRELRYDSTCERLYYGDDEKYFRKPY NFLKYHFEALADTGISSEFYDNANDLLSKVKKDKSDSFGVTIGIGPAGSPLLVGVGVSHSQDTSFLNELN KYNEKKFIFTRIFTKVQTAHFKMRKDDIMLDEGMLQSLMELPDQYNYGMYAKFINDYGTHYITSGSMGGI YEYILVIDKAKMESLGITSRDITTCFGGSLGIQYEDKINVGGGLSGDHCKKFGGGKTERARKAMAVEDII SRVRGGSSGWSGGLAQNRSTITYRSWGRSLKYNPVVIDFEMQPIHEVLRHTSLGPLEAKRQNLRRALDQY LMEFNACRCGPCFNNGVPILEGTSCRCQCRLGSLGAACEQTQTEGAKADGSWSCWSSWSVCRAGIQERRR ECDNPAPQNGGASCPGRKVQTQAC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000562 |
ORF Size | 1752 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000562.1, NM_000562.2, NP_000553.1 |
RefSeq Size | 2392 bp |
RefSeq ORF | 1755 bp |
Locus ID | 731 |
Cytogenetics | 1p32.2 |
Domains | tsp_1, MACPF, ldl_recept_a |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus |
MW | 65.2 kDa |
Gene Summary | 'C8 is a component of the complement system and contains three polypeptides, alpha, beta and gamma. This gene encodes the alpha subunit of C8. C8 participates in the formation of the membrane attack complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause complement C8 alpha-gamma deficiency. [provided by RefSeq, Nov 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119839 | C8A (untagged)-Human complement component 8, alpha polypeptide (C8A) |
USD 760.00 |
|
RG221824 | C8A (GFP-tagged) - Human complement component 8, alpha polypeptide (C8A) |
USD 530.00 |
|
RC221824L3 | Lenti ORF clone of Human complement component 8, alpha polypeptide (C8A), Myc-DDK-tagged |
USD 680.00 |
|
RC221824L4 | Lenti ORF clone of Human complement component 8, alpha polypeptide (C8A), mGFP tagged |
USD 680.00 |
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