FCRL2 (NM_030764) Human Tagged ORF Clone

CAT#: RC221878

FCRL2 (Myc-DDK-tagged)-Human Fc receptor-like 2 (FCRL2)

Product Images

Western blot validation of overexpression lysate (Cat# LY410726) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221878 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FCRL2 protein (Cat# TP321878). The protein was produced from HEK293T cells transfected with FCRL2 cDNA clone (Cat# RC221878) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FCRL2
• Synonyms: CD307b; FCRH2; IFGP4; IRTA4; SPAP1; SPAP1A; SPAP1B; SPAP1C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221878 representing NM_030764
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGTGGTCATTGCTGGTCATCTTTGATGCAGTCACTGAACAGGCAGATTCGCTGACCCTTGTGG
  CGCCCTCTTCTGTCTTCGAAGGAGACAGCATCGTTCTGAAATGCCAGGGAGAACAGAACTGGAAAATTCA
  GAAGATGGCTTACCATAAGGATAACAAAGAGTTATCTGTTTTCAAAAAATTCTCAGATTTCCTTATCCAA
  AGTGCAGTTTTAAGTGACAGTGGTAACTATTTCTGTAGTACCAAAGGACAACTCTTTCTCTGGGATAAAA
  CTTCAAATATAGTAAAGATAAAAGTCCAAGAGCTCTTTCAACGTCCTGTGCTGACTGCCAGCTCCTTCCA
  GCCCATCGAAGGGGGTCCAGTGAGCCTGAAATGTGAGACCCGGCTCTCTCCACAGAGGTTGGATGTTCAA
  CTCCAGTTCTGCTTCTTCAGAGAAAACCAGGTCCTGGGGTCAGGCTGGAGCAGCTCTCCGGAGCTCCAGA
  TTTCTGCCGTGTGGAGTGAAGACACAGGGTCTTACTGGTGCAAGGCAGAAACGGTGACTCACAGGATCAG
  AAAACAGAGCCTCCAATCCCAGATTCACGTGCAGAGAATCCCCATCTCTAATGTAAGCTTGGAGATCCGG
  GCCCCCGGGGGACAGGTGACTGAAGGACAAAAACTGATCCTGCTCTGCTCAGTGGCTGGGGGTACAGGAA
  ATGTCACATTCTCCTGGTACAGAGAGGCCACAGGAACCAGTATGGGAAAGAAAACCCAGCGTTCCCTGTC
  AGCAGAGCTGGAGATCCCAGCTGTGAAAGAGAGTGATGCCGGCAAATATTACTGTAGAGCTGACAACGGC
  CATGTGCCTATCCAGAGCAAGGTGGTGAATATCCCTGTGAGAATTCCAGTGTCTCGCCCTGTCCTCACCC
  TCAGGTCTCCTGGGGCCCAGGCTGCAGTGGGGGACCTGCTGGAGCTTCACTGTGAGGCCCTGAGAGGCTC
  TCCCCCAATCTTGTACCAATTTTATCATGAGGATGTCACCCTTGGGAACAGCTCGGCCCCCTCTGGAGGA
  GGGGCCTCCTTCAACCTCTCTTTGACTGCAGAACATTCTGGAAACTACTCCTGTGAGGCCAACAACGGCC
  TGGGGGCCCAGTGCAGTGAGGCAGTGCCAGTCTCCATCTCAGGACCTGATGGCTATAGAAGAGACCTCAT
  GACAGCTGGAGTTCTCTGGGGACTGTTTGGTGTCCTTGGTTTCACTGGTGTTGCTTTGCTGTTGTATGCC
  TTGTTCCACAAGATATCAGGAGAAAGTTCTGCCACTAATGAACCCAGAGGGGCTTCCAGGCCAAATCCTC
  AAGAGTTCACCTATTCAAGCCCAACCCCAGACATGGAGGAGCTGCAGCCAGTGTATGTCAATGTGGGCTC
  TGTAGATGTGGATGTGGTTTATTCTCAGGTCTGGAGCATGCAGCAGCCAGAAAGCTCAGCAAACATCAGG
  ACACTTCTGGAGAACAAGGACTCCCAAGTCATCTACTCTTCTGTGAAGAAATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221878 representing NM_030764
  Red=Cloning site Green=Tags(s)
  
  MLLWSLLVIFDAVTEQADSLTLVAPSSVFEGDSIVLKCQGEQNWKIQKMAYHKDNKELSVFKKFSDFLIQ
  SAVLSDSGNYFCSTKGQLFLWDKTSNIVKIKVQELFQRPVLTASSFQPIEGGPVSLKCETRLSPQRLDVQ
  LQFCFFRENQVLGSGWSSSPELQISAVWSEDTGSYWCKAETVTHRIRKQSLQSQIHVQRIPISNVSLEIR
  APGGQVTEGQKLILLCSVAGGTGNVTFSWYREATGTSMGKKTQRSLSAELEIPAVKESDAGKYYCRADNG
  HVPIQSKVVNIPVRIPVSRPVLTLRSPGAQAAVGDLLELHCEALRGSPPILYQFYHEDVTLGNSSAPSGG
  GASFNLSLTAEHSGNYSCEANNGLGAQCSEAVPVSISGPDGYRRDLMTAGVLWGLFGVLGFTGVALLLYA
  LFHKISGESSATNEPRGASRPNPQEFTYSSPTPDMEELQPVYVNVGSVDVDVVYSQVWSMQQPESSANIR
  TLLENKDSQVIYSSVKKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030764
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030764.2, NM_030764.3, NP_110391.2
• RefSeq Size: 2573 bp
• RefSeq ORF: 1527 bp
• Locus ID: 79368
• Protein Families: Druggable Genome, Transmembrane
• MW: 53.4 kDa
• Gene Summary: This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains one immunoreceptor-tyrosine activation motif and two immunoreceptor-tyrosine inhibitory motifs. This protein may be a prognostic marker for chronic lymphocytic leukemia. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Apr 2009]