INPP5A (NM_005539) Human Tagged ORF Clone
CAT#: RC222083
INPP5A (Myc-DDK-tagged)-Human inositol polyphosphate-5-phosphatase, 40kDa (INPP5A)
"NM_005539" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | INPP5A |
Synonyms | 5PTASE |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222083 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGAAGGCGGCCGCCCCGGGCACCGCGGTGCTGCTGGTCACGGCCAACGTGGGCTCGCTCTTCG ACGACCCAGAAAACCTGCAGAAGAACTGGCTTCGGGAATTTTACCAGGTCGTGCACACACACAAGCCGCA CTTCATGGCCTTGCACTGTCAGGAGTTTGGAGGGAAGAACTACGAGGCCTCCATGTCCCACGTGGACAAG TTCGTCAAAGAACTATTGTCGAGTGATGCGATGAAAGAATATAACAGGGCTCGAGTCTACCTGGATGAAA ACTACAAATCCCAGGAGCACTTCACGGCACTAGGAAGCTTTTATTTTCTTCATGAGTCCTTAAAAAACAT CTACCAGTTTGACTTTAAAGCTAAGAAGTATAGAAAGGTCGCTGGCAAAGAGATCTACTCGGATACCTTA GAGAGCACGCCCATGCTGGAGAAGGAGAAGTTTCCGCAGGACTACTTCCCCGAGTGCAAATGGTCAAGAA AAGGCTTCATCCGGACGAGGTGGTGCATTGCAGACTGTGCCTTTGACTTGGTGAATATCCATCTTTTCCA TGATGCTTCCAATCTGGTCGCCTGGGAAACAAGCCCTTCCGTGTACTCGGGAATCCGGCACAAGGCACTG GGCTACGTGCTGGACAGAATCATTGATCAGCGATTCGAGAAGGTTTCCTACTTTGTATTTGGTGATTTCA ACTTCCGGCTGGATTCCAAGTCCGTCGTGGAGACGCTCTGCACAAAAGCCACCATGCAGACGGTCCGGGC CGCCGACACCAATGAAGTGGTGAAGCTCATATTTCGTGAGTCGGACAACGACCGGAAGGTTATGCTCCAG TTAGAAAAGAAACTCTTCGACTACTTCAACCAGGAGGTTTTCCGAGACAACAACGGCACCGCGCTCTTGG AGTTTGACAAGGAGTTGTCTGTCTTTAAGGACAGACTGTATGAACTGGACATCTCGTTCCCTCCCAGCTA CCCGTACAGTGAGGACGCCCGCCAGGGTGAGCAGTACATGAACACCCGGTGCCCAGCCTGGTGTGACCGC ATCCTCATGTCCCCGTCTGCCAAGGAGCTGGTGCTGCGGTCGGAGAGCGAGGAGAAGGTTGTCACCTATG ACCACATTGGGCCCAACGTCTGCATGGGAGACCACAAGCCCGTGTTCCTGGCCTTCCGAATCATGCCCGG GGCAGGTAAACCTCATGCCCATGTGCACAAGTGTTGTGTCGTGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222083 protein sequence
Red=Cloning site Green=Tags(s) MAGKAAAPGTAVLLVTANVGSLFDDPENLQKNWLREFYQVVHTHKPHFMALHCQEFGGKNYEASMSHVDK FVKELLSSDAMKEYNRARVYLDENYKSQEHFTALGSFYFLHESLKNIYQFDFKAKKYRKVAGKEIYSDTL ESTPMLEKEKFPQDYFPECKWSRKGFIRTRWCIADCAFDLVNIHLFHDASNLVAWETSPSVYSGIRHKAL GYVLDRIIDQRFEKVSYFVFGDFNFRLDSKSVVETLCTKATMQTVRAADTNEVVKLIFRESDNDRKVMLQ LEKKLFDYFNQEVFRDNNGTALLEFDKELSVFKDRLYELDISFPPSYPYSEDARQGEQYMNTRCPAWCDR ILMSPSAKELVLRSESEEKVVTYDHIGPNVCMGDHKPVFLAFRIMPGAGKPHAHVHKCCVVQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_005539 |
ORF Size | 1236 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005539.3, NM_005539.4, NP_005530.3 |
RefSeq Size | 2938 bp |
RefSeq ORF | 1239 bp |
Locus ID | 3632 |
Cytogenetics | 10q26.3 |
Domains | IPPc, Exo_endo_phos |
Protein Families | Druggable Genome |
Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
MW | 47.8 kDa |
Gene Summary | 'The protein encoded by this gene is a membrane-associated type I inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC317660 | INPP5A (untagged)-Human inositol polyphosphate-5-phosphatase, 40kDa (INPP5A) |
USD 760.00 |
|
RG222083 | INPP5A (GFP-tagged) - Human inositol polyphosphate-5-phosphatase, 40kDa (INPP5A) |
USD 460.00 |
|
RC222083L3 | Lenti ORF clone of Human inositol polyphosphate-5-phosphatase, 40kDa (INPP5A), Myc-DDK-tagged |
USD 620.00 |
|
RC222083L4 | Lenti ORF clone of Human inositol polyphosphate-5-phosphatase, 40kDa (INPP5A), mGFP tagged |
USD 620.00 |
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