MED1 (NM_004774) Human Tagged ORF Clone
CAT#: RC222118
MED1 (Myc-DDK-tagged)-Human mediator complex subunit 1 (MED1)
"NM_004774" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MED1 |
Synonyms | CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222118 representing NM_004774
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGCTCAGGGGGAAACCGAGGAGTCAGAAAAGCTGAGTAAGATGAGTTCTCTCCTGGAACGGCTCC ATGCAAAATTTAACCAAAATAGACCCTGGAGTGAAACCATTAAGCTTGTGCGTCAAGTCATGGAGAAGAG GGTTGTGATGAGTTCTGGAGGGCATCAACATTTGGTCAGCTGTTTGGAGACATTGCAGAAGGCTCTCAAA GTAACATCTTTACCAGCAATGACTGATCGTTTGGAGTCCATAGCAAGACAGAATGGACTGGGCTCTCATC TCAGTGCCAGTGGCACTGAATGTTACATCACGTCAGATATGTTCTATGTGGAAGTGCAGTTAGATCCTGC AGGACAGCTTTGTGATGTAAAAGTGGCTCACCATGGGGAGAATCCTGTGAGCTGTCCGGAGCTTGTACAG CAGCTAAGGGAAAAAAATTTTGATGAATTTTCTAAGCACCTTAAGGGCCTTGTTAATCTGTATAACCTTC CAGGGGACAACAAACTGAAGACTAAAATGTACTTGGCTCTCCAATCCTTAGAACAAGATCTTTCTAAAAT GGCAATTATGTACTGGAAAGCAACTAATGCTGGTCCCTTGGATAAGATTCTTCATGGAAGTGTTGGCTAT CTCACACCAAGGAGTGGGGGTCATTTAATGAACCTGAAGTACTATGTCTCTCCTTCTGACCTACTGGATG ACAAGACTGCATCTCCCATCATTTTGCATGAGAATAATGTTTCTCGATCTTTGGGCATGAATGCATCAGT GACAATTGAAGGAACATCTGCTGTGTACAAACTCCCAATTGCACCATTAATTATGGGGTCACATCCAGTT GACAATAAATGGACCCCTTCCTTCTCCTCAATCACCAGTGCCAACAGTGTTGATCTTCCTGCCTGTTTCT TCTTGAAATTTCCCCAGCCAATCCCAGTATCTAGAGCATTTGTTCAGAAACTGCAGAACTGCACAGGAAT TCCATTGTTTGAAACTCAACCAACTTATGCACCCCTGTATGAACTGATCACTCAGTTTGAGCTATCAAAG GACCCTGACCCCATACCTTTGAATCACAACATGAGATTTTATGCTGCTCTTCCTGGTCAGCAGCACTGCT ATTTCCTCAACAAGGATGCTCCTCTTCCAGATGGCCGAAGTCTACAGGGAACCCTTGTTAGCAAAATCAC CTTTCAGCACCCTGGCCGAGTTCCTCTTATCCTAAATCTGATCAGACACCAAGTGGCCTATAACACCCTC ATTGGAAGCTGTGTCAAAAGAACTATTCTGAAAGAAGATTCTCCTGGGCTTCTCCAATTTGAAGTGTGTC CTCTCTCAGAGTCTCGTTTCAGCGTATCTTTTCAGCACCCTGTGAATGACTCCCTGGTGTGTGTGGTAAT GGATGTGCAGGACTCAACACATGTGAGCTGTAAACTCTACAAAGGGCTGTCGGATGCACTGATCTGCACA GATGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATCCCTGTGACGATGAGGGCTATTCGGAGGA AAGCTGAAACCATTCAAGCCGACACCCCAGCACTGTCCCTCATTGCAGAGACAGTTGAAGACATGGTGAA AAAGAACCTGCCCCCGGCTAGCAGCCCAGGGTATGGCATGACCACAGGCAACAACCCAATGAGTGGTACC ACTACACCAACCAACACCTTTCCGGGGGGTCCCATTACCACCTTGTTTAATATGAGCATGAGCATCAAAG ATCGGCATGAGTCGGTGGGCCATGGGGAGGACTTCAGCAAGGTGTCTCAGAACCCAATTCTTACCAGTTT GTTGCAAATCACAGGGAACGGGGGGTCTACCATTGGCTCGAGTCCGACCCCTCCTCATCACACGCCGCCA CCTGTCTCTTCGATGGCCGGCAACACCAAGAACCACCCGATGCTCATGAACCTTCTTAAAGATAATCCTG CCCAGGATTTCTCAACCCTTTATGGAAGCAGCCCTTTAGAAAGGCAGAACTCCTCTTCCGGCTCACCCCG CATGGAAATATGCTCGGGGAGCAACAAGACCAAGAAAAAGAAGTCATCAAGATTACCACCTGAGAAACCA AAGCACCAGACTGAAGATGACTTTCAGAGGGAGCTATTTTCAATGGATGTTGACTCACAGAACCCTATCT TTGATGTCAACATGACAGCTGACACGCTGGATACGCCACACATCACTCCAGCTCCAAGCCAGTGTAGCAC TCCCCCAACAACTTACCCACAACCAGTACCTCACCCCCAACCCAGTATTCAAAGGATGGTCCGACTATCC AGTTCAGACAGCATTGGCCCAGATGTAACTGACATCCTTTCAGACATTGCAGAAGAAGCTTCTAAACTTC CCAGCACTAGTGATGATTGCCCAGCCATTGGCACCCCTCTTCGAGATTCTTCAAGCTCTGGGCATTCTCA GAGTACCCTGTTTGACTCTGATGTCTTTCAAACTAACAATAATGAAAATCCATACACTGATCCAGCTGAT CTTATTGCAGATGCTGCTGGAAGCCCCAGTAGTGACTCTCCTACCAATCATTTTTTTCATGATGGAGTAG ATTTCAATCCTGATTTATTGAACAGCCAGAGCCAAAGTGGTTTTGGAGAAGAATATTTTGATGAAAGCAG CCAAAGTGGGGATAATGATGATTTCAAAGGATTTGCATCTCAGGCACTAAATACTTTGGGGGTGCCAATG CTTGGAGGTGATAATGGGGAGACCAAGTTTAAGGGCAATAACCAAGCCGACACAGTTGATTTCAGTATTA TTTCAGTAGCCGGCAAAGCTTTAGCTCCTGCAGATCTTATGGAGCATCACAGTGGTAGTCAGGGTCCTTT ACTGACCACTGGGGACTTAGGGAAAGAAAAGACTCAAAAGAGGGTAAAGGAAGGCAATGGCACCAGTAAT AGTACTCTCTCGGGGCCCGGATTAGACAGCAAACCAGGGAAGCGCAGTCGGACCCCTTCTAATGATGGGA AAAGCAAAGATAAGCCTCCAAAGCGGAAGAAGGCAGACACTGAGGGAAAGTCTCCATCTCATAGTTCTTC TAACAGACCTTTTACCCCACCTACCAGTACAGGTGGATCTAAATCGCCAGGCAGTGCAGGAAGATCTCAG ACTCCCCCAGGTGTTGCCACACCACCCATTCCCAAAATCACTATTCAGATTCCTAAGGGAACAGTGATGG TGGGCAAGCCTTCCTCTCACAGTCAGTATACCAGCAGTGGTTCTGTGTCTTCCTCAGGCAGCAAAAGCCA CCATAGCCATTCTTCCTCCTCTTCCTCATCTGCTTCCACCTCAGGGAAGATGAAAAGCAGTAAATCAGAA GGTTCATCAAGTTCCAAGTTAAGTAGCAGTATGTATTCTAGCCAGGGGTCTTCTGGATCTAGCCAGTCCA AAAATTCATCCCAGTCTGGGGGGAAGCCAGGCTCCTCTCCCATAACCAAGCATGGACTGAGCAGTGGCTC TAGCAGCACCAAGATGAAACCTCAAGGAAAGCCATCATCACTTATGAATCCTTCTTTAAGTAAACCAAAC ATATCCCCTTCTCATTCAAGGCCACCTGGAGGCTCTGACAAGCTTGCCTCTCCAATGAAGCCTGTTCCTG GAACTCCTCCATCCTCTAAAGCCAAGTCCCCTATCAGTTCAGGTTCTGGTGGTTCTCATATGTCTGGAAC TAGTTCAAGCTCTGGCATGAAGTCATCTTCAGGGTTAGGATCCTCAGGCTCGTTGTCCCAGAAAACTCCC CCATCATCTAATTCCTGTACGGCATCTTCCTCCTCCTTTTCCTCAAGTGGCTCTTCCATGTCATCCTCTC AGAACCAGCATGGGAGTTCTAAAGGAAAATCTCCCAGCAGAAACAAGAAGCCGTCCTTGACAGCTGTCAT AGATAAACTGAAGCATGGGGTTGTCACCAGTGGCCCTGGGGGTGAAGACCCACTGGACGGCCAGATGGGG GTGAGCACAAATTCTTCCAGCCATCCTATGTCCTCCAAACATAACATGTCAGGAGGAGAGTTTCAGGGCA AGCGTGAGAAAAGTGATAAAGACAAATCAAAGGTTTCCACCTCCGGGAGTTCAGTGGATTCTTCTAAGAA GACCTCAGAGTCAAAAAATGTGGGGAGCACAGGTGTGGCAAAAATTATCATCAGTAAGCATGATGGAGGC TCCCCTAGCATTAAAGCCAAAGTGACTTTGCAGAAACCTGGGGAAAGTAGTGGAGAAGGGCTTAGGCCTC AAATGGCTTCTTCTAAAAACTATGGCTCTCCACTCATCAGTGGTTCCACTCCAAAGCATGAGCGTGGCTC TCCCAGCCATAGTAAGTCACCAGCATATACCCCCCAGAATCTGGACAGTGAAAGTGAGTCAGGCTCCTCC ATAGCAGAGAAATCTTATCAGAATAGTCCCAGCTCAGACGATGGTATCCGACCACTTCCAGAATACAGCA CAGAGAAACATAAGAAGCACAAAAAGGAAAAGAAGAAAGTAAAAGACAAAGATAGGGACCGAGACCGGGA CAAAGACCGAGACAAGAAAAAATCTCATAGCATCAAGCCAGAGAGTTGGTCCAAATCACCCATCTCTTCA GACCAGTCCTTGTCTATGACAAGTAACACAATCTTATCTGCAGACAGACCCTCAAGGCTCAGCCCAGACT TTATGATTGGGGAGGAAGATGATGATCTTATGGATGTGGCCCTGATTGGGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222118 representing NM_004774
Red=Cloning site Green=Tags(s) MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALK VTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQ QLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGY LTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPV DNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSK DPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTL IGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICT DDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGT TTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPP PVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKP KHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLS SSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPAD LIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPM LGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSN STLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQ TPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSE GSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPN ISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTP PSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMG VSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGG SPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSS IAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISS DQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004774 |
ORF Size | 4743 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004774.2, NM_004774.3, NP_004765.2 |
RefSeq Size | 5809 bp |
RefSeq ORF | 4746 bp |
Locus ID | 5469 |
Cytogenetics | 17q12 |
Protein Families | Druggable Genome, Nuclear Hormone Receptor, Transcription Factors |
MW | 168.3 kDa |
Gene Summary | 'The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC309905 | MED1 (untagged)-Human mediator complex subunit 1 (MED1) |
USD 1,100.00 |
|
RG222118 | MED1 (GFP-tagged) - Human mediator complex subunit 1 (MED1) |
USD 1,400.00 |
|
RC222118L1 | Lenti ORF clone of Human mediator complex subunit 1 (MED1), Myc-DDK-tagged |
USD 1,824.00 |
|
RC222118L2 | Lenti ORF clone of Human mediator complex subunit 1 (MED1), mGFP tagged |
USD 1,470.00 |
|
RC222118L3 | Lenti ORF clone of Human mediator complex subunit 1 (MED1), Myc-DDK-tagged |
USD 1,824.00 |
|
RC222118L4 | Lenti ORF clone of Human mediator complex subunit 1 (MED1), mGFP tagged |
USD 1,470.00 |
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