KCNH6 (NM_173092) Human Tagged ORF Clone

CAT#: RC222145

KCNH6 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 6 (KCNH6), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406688) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222145 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNH6
• Synonyms: ERG-2; ERG2; hERG-2; HERG2; Kv11.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222145 representing NM_173092
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTCCGCAGGGGCCACGTCGCTCCCCAAAACACTTACCTGGACACCATCATCCGCAAGTTCGAGG
  GCCAAAGTCGGAAGTTCCTGATTGCCAATGCTCAGATGGAGAACTGCGCCATCATTTACTGCAACGACGG
  CTTCTGCGAACTCTTCGGCTACTCCCGAGTGGAGGTGATGCAGCAACCCTGCACCTGCGACTTCCTCACA
  GGCCCCAACACACCAAGCAGCGCCGTGTCCCGCCTAGCGCAGGCCCTGCTGGGGGCTGAGGAGTGCAAGG
  TGGACATCCTCTACTACCGCAAGGATGCCTCCAGCTTCCGCTGCCTGGTAGATGTGGTGCCCGTGAAGAA
  CGAGGACGGGGCTGTCATCATGTTCATTCTCAACTTCGAGGACCTGGCCCAGCTCCTGGCCAAGTGCAGC
  AGCCGCAGCTTGTCCCAGCGCCTGTTGTCCCAGAGCTTCCTGGGCTCCGAGGGCTCTCACGGCAGGCCAG
  GCGGACCAGGGCCAGGCACAGGCAGGGGCAAGTACAGGACCATCAGCCAGATCCCACAGTTCACGCTCAA
  CTTCGTGGAGTTCAACTTGGAGAAGCACCGCTCCAGCTCCACCACGGAGATTGAGATCATCGCGCCCCAT
  AAGGTGGTGGAGCGGACACAGAACGTCACTGAGAAGGTCACCCAGGTCCTGTCCCTGGGCGCGGATGTGC
  TGCCGGAGTACAAGCTGCAGGCGCCGCGCATCCACCGCTGGACCATCCTGCACTACAGCCCCTTCAAGGC
  CGTGTGGGACTGGCTCATCCTGCTGCTGGTCATCTACACGGCTGTCTTCACGCCCTACTCAGCCGCCTTC
  CTGCTCAGCGACCAGGACGAATCACGGCGTGGGGCCTGCAGCTATACCTGCAGTCCCCTCACTGTGGTGG
  ATCTCATCGTGGACATCATGTTCGTCGTGGACATCGTCATCAACTTCCGCACCACCTATGTCAACACCAA
  TGATGAGGTGGTCAGCCACCCCCGCCGCATCGCCGTCCACTACTTCAAGGGCTGGTTCCTCATTGACATG
  GTGGCCGCCATCCCTTTCGACCTCCTGATCTTCCGCACTGGCTCCGATGAGACCACAACCCTGATTGGGC
  TATTGAAGACAGCGCGGCTGCTGCGGCTGGTGCGCGTAGCACGGAAGCTGGACCGCTACTCTGAGTATGG
  GGCGGCTGTGCTCTTCTTGCTCATGTGCACCTTCGCGCTCATAGCGCACTGGCTGGCCTGCATCTGCAGC
  CTCACCAGCGTGGGCTTCGGCAATGTCTCGCCCAACACCAACTCCGAGAAGGTCTTCTCCATCTGCGTCA
  TGCTCATCGGCTCCCTGATGTACGCCAGCATCTTCGGGAACGTGTCCGCGATCATCCAGCGCCTGTACTC
  GGGCACCGCGCGCTACCACACGCAGATGCTGCGTGTCAAGGAGTTCATCCGCTTCCACCAGATCCCCAAC
  CCACTGCGCCAGCGCCTGGAGGAGTATTTCCAGCACGCCTGGTCCTACACCAATGGCATTGACATGAACG
  CGGTGCTGAAGGGCTTCCCCGAGTGCCTGCAGGCTGACATCTGCCTGCACCTGCACCGCGCACTGCTGCA
  GCACTGCCCAGCTTTCAGCGGCGCCGGCAAGGGCTGCCTGCGCGCGCTAGCCGTCAAGTTCAAGACCACC
  CACGCGCCGCCTGGGGACACGCTGGTGCACCTCGGCGACGTGCTCTCCACCCTCTACTTCATCTCCCGAG
  GCTCCATCGAGATCCTGCGCGACGACGTGGTCGTGGCCATCCTAGGAAAGAATGACATCTTTGGGGAACC
  CGTCAGCCTCCATGCCCAGCCAGGCAAGTCCAGTGCAGACGTGCGGGCTCTGACCTACTGCGACCTGCAC
  AAGATCCAGCGGGCAGATCTGCTGGAGGTGCTGGACATGTACCCGGCCTTTGCGGAGAGCTTCTGGAGTA
  AGCTGGAGGTCACCTTCAACCTGCGGGACGCAGCCGGGGGTCTCCACTCATCCCCCCGACAGGCTCCTGG
  CAGCCAAGACCACCAAGGTTTCTTTCTCAGTGACAACCAGTCAGATGCAGCCCCTCCCCTGAGCATCTCA
  GATGCATCTGGCCTCTGGCCTGAGCTACTGCAGGAAATGCCCCCAAGGCACAGCCCCCAAAGCCCTCAGG
  AAGACCCAGATTGCTGGCCTCTGAAGCTGGGCTCCAGGCTAGAGCAGCTCCAGGCCCAGATGAACAGGCT
  GGAGTCCCGCGTGTCCTCAGACCTCAGCCGCATCTTGCAGCTCCTCCAGAAGCCCATGCCCCAGGGCCAC
  GCCAGCTACATTCTGGAAGCCCCTGCCTCCAATGACCTGGCCTTGGTTCCTATAGCCTCGGAGACGACGA
  GTCCAGGGCCCAGGCTGCCCCAGGGCTTTCTGCCTCCTGCACAGACCCCAAGCTATGGAGACTTGGATGA
  CTGTAGTCCAAAGCACAGGAACTCCTCCCCCAGGATGCCTCACCTGGCTGTGGCAACGGACAAAACTCTG
  GCACCATCCTCAGAACAGGAACAGCCTGAGGGGCTCTGGCCACCCCTAGCCTCACCTCTACATCCCCTGG
  AAGTACAAGGACTCATCTGTGGTCCCTGCTTCTCCTCCCTCCCTGAACACCTTGGCTCTGTTCCCAAGCA
  GCTGGACTTCCAGAGACATGGCTCAGATCCTGGATTTGCAGGGAGTTGGGGCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222145 representing NM_173092
  Red=Cloning site Green=Tags(s)
  
  MPVRRGHVAPQNTYLDTIIRKFEGQSRKFLIANAQMENCAIIYCNDGFCELFGYSRVEVMQQPCTCDFLT
  GPNTPSSAVSRLAQALLGAEECKVDILYYRKDASSFRCLVDVVPVKNEDGAVIMFILNFEDLAQLLAKCS
  SRSLSQRLLSQSFLGSEGSHGRPGGPGPGTGRGKYRTISQIPQFTLNFVEFNLEKHRSSSTTEIEIIAPH
  KVVERTQNVTEKVTQVLSLGADVLPEYKLQAPRIHRWTILHYSPFKAVWDWLILLLVIYTAVFTPYSAAF
  LLSDQDESRRGACSYTCSPLTVVDLIVDIMFVVDIVINFRTTYVNTNDEVVSHPRRIAVHYFKGWFLIDM
  VAAIPFDLLIFRTGSDETTTLIGLLKTARLLRLVRVARKLDRYSEYGAAVLFLLMCTFALIAHWLACICS
  LTSVGFGNVSPNTNSEKVFSICVMLIGSLMYASIFGNVSAIIQRLYSGTARYHTQMLRVKEFIRFHQIPN
  PLRQRLEEYFQHAWSYTNGIDMNAVLKGFPECLQADICLHLHRALLQHCPAFSGAGKGCLRALAVKFKTT
  HAPPGDTLVHLGDVLSTLYFISRGSIEILRDDVVVAILGKNDIFGEPVSLHAQPGKSSADVRALTYCDLH
  KIQRADLLEVLDMYPAFAESFWSKLEVTFNLRDAAGGLHSSPRQAPGSQDHQGFFLSDNQSDAAPPLSIS
  DASGLWPELLQEMPPRHSPQSPQEDPDCWPLKLGSRLEQLQAQMNRLESRVSSDLSRILQLLQKPMPQGH
  ASYILEAPASNDLALVPIASETTSPGPRLPQGFLPPAQTPSYGDLDDCSPKHRNSSPRMPHLAVATDKTL
  APSSEQEQPEGLWPPLASPLHPLEVQGLICGPCFSSLPEHLGSVPKQLDFQRHGSDPGFAGSWGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173092
• ORF Size: 2715 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173092.1, NM_173092.2, NP_775115.1
• RefSeq Size: 3017 bp
• RefSeq ORF: 2718 bp
• Locus ID: 81033
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 100.3 kDa
• Gene Summary: Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]