ZNF161 (VEZF1) (NM_007146) Human Tagged ORF Clone

CAT#: RC222182

VEZF1 (Myc-DDK-tagged)-Human vascular endothelial zinc finger 1 (VEZF1)

Product Images

Western blot validation of overexpression lysate (Cat# LY416152) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222182 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VEZF1
• Synonyms: DB1; ZNF161
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222182 representing NM_007146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCAACTGGACCGCGTTCCTGTTCCAGGCCCATGAAGCTTCCCATCACCAACAGCAGGCAGCAC
  AGAACAGCTTGCTGCCCCTCCTGAGCTCTGCCGTGGAGCCCCCTGATCAGAAACCATTGCTTCCAATACC
  AATAACTCAGAAACCTCAGGGTGCACCAGAAACATTAAAGGATGCCATTGGGATTAAAAAAGAAAAACCC
  AAAACTTCATTTGTGTGCACTTACTGCAGTAAAGCTTTCAGGGACAGCTATCACCTGAGGCGCCACGAAT
  CCTGCCACACAGGGATCAAGTTGGTGTCCCGGCCAAAGAAAACCCCCACCACGGTGGTTCCCCTTATCTC
  TACCATCGCTGGGGACAGCAGCCGAACTTCGTTGGTCTCGACCATTGCAGGCATCTTGTCAACAGTCACT
  ACATCTTCCTCGGGCACCAACCCCAGTAGCAGTGCCAGCACCACAGCTATGCCAGTGACCCAGTCTGTCA
  AGAAACCCAGTAAGCCTGTCAAGAAGAACCATGCTTGTGAGATGTGTGGGAAGGCCTTCCGAGATGTGTA
  CCATCTCAATCGACACAAGCTCTCCCATTCAGATGAGAAACCCTTTGAGTGTCCTATTTGTAATCAGCGC
  TTCAAGAGGAAGGACCGGATGACTTACCATGTGAGGTCTCATGAAGGAGGCATCACCAAACCCTATACTT
  GCAGTGTTTGTGGGAAAGGCTTCTCAAGGCCTGACCACTTAAGCTGTCATGTAAAACATGTCCATTCAAC
  AGAAAGACCCTTCAAATGCCAAACGTGCACTGCTGCCTTTGCCACCAAAGACAGACTGCGGACACACATG
  GTGCGCCATGAAGGCAAGGTATCATGTAACATCTGTGGGAAGCTCCTGAGTGCAGCATACATCACCAGCC
  ACTTAAAGACTCATGGGCAGAGCCAAAGTATCAACTGTAATACATGTAAACAAGGCATCAGTAAAACATG
  CATGAGTGAAGAGACCAGTAACCAAAAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAA
  CAACAACAACAACATGTGACAAGCTGGCCAGGGAAGCAAGTAGAAACACTGAGACTGTGGGAAGAAGCTG
  TTAAAGCAAGGAAGAAAGAAGCTGCTAACCTGTGCCAAACCTCCACGGCTGCTACGACACCTGTGACTCT
  CACTACTCCATTCAGTATAACATCCTCTGTGTCGTCTGGGACTATGTCAAACCCAGTCACAGTGGCAGCT
  GCAATGAGCATGAGAAGTCCAGTAAATGTTTCAAGTGCAGTTAACATAACCAGCCCAATGAACATAGGGC
  ATCCTGTAACTATAACCAGTCCATTATCCATGACCTCTCCTTTAACACTCACTACCCCAGTCAACCTCCC
  CACCCCCGTCACTGCCCCAGTGAATATAGCACACCCTGTCACCATCACATCTCCAATGAATCTACCCACA
  CCTATGACATTAGCCGCCCCTCTCAATATAGCAATGAGACCTGTAGAGAGCATGCCTTTCTTGCCCCAAG
  CTTTGCCTACATCACCGCCTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222182 representing NM_007146
  Red=Cloning site Green=Tags(s)
  
  MEANWTAFLFQAHEASHHQQQAAQNSLLPLLSSAVEPPDQKPLLPIPITQKPQGAPETLKDAIGIKKEKP
  KTSFVCTYCSKAFRDSYHLRRHESCHTGIKLVSRPKKTPTTVVPLISTIAGDSSRTSLVSTIAGILSTVT
  TSSSGTNPSSSASTTAMPVTQSVKKPSKPVKKNHACEMCGKAFRDVYHLNRHKLSHSDEKPFECPICNQR
  FKRKDRMTYHVRSHEGGITKPYTCSVCGKGFSRPDHLSCHVKHVHSTERPFKCQTCTAAFATKDRLRTHM
  VRHEGKVSCNICGKLLSAAYITSHLKTHGQSQSINCNTCKQGISKTCMSEETSNQKQQQQQQQQQQQQQQ
  QQQQHVTSWPGKQVETLRLWEEAVKARKKEAANLCQTSTAATTPVTLTTPFSITSSVSSGTMSNPVTVAA
  AMSMRSPVNVSSAVNITSPMNIGHPVTITSPLSMTSPLTLTTPVNLPTPVTAPVNIAHPVTITSPMNLPT
  PMTLAAPLNIAMRPVESMPFLPQALPTSPPW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007146
• ORF Size: 1563 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007146.2, NP_009077.2
• RefSeq Size: 4652 bp
• RefSeq ORF: 1566 bp
• Locus ID: 7716
• Cytogenetics: 17q22
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• MW: 56.8 kDa
• Gene Summary: 'Transcriptional regulatory proteins containing tandemly repeated zinc finger domains are thought to be involved in both normal and abnormal cellular proliferation and differentiation. ZNF161 is a C2H2-type zinc finger protein (Koyano-Nakagawa et al., 1994 [PubMed 8035792]). See MIM 603971 for general information on zinc finger proteins.[supplied by OMIM, Sep 2008]'