plasticity related gene 3 (PLPPR1) (NM_207299) Human Tagged ORF Clone

CAT#: RC222205

LPPR1 (Myc-DDK-tagged)-Human lipid phosphate phosphatase-related protein type 1 (LPPR1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY404081) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222205 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PLPPR1 protein (Cat# TP322205). The protein was produced from HEK293T cells transfected with PLPPR1 cDNA clone (Cat# RC222205) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLPPR1
• Synonyms: LPPR1; PRG-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222205 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTAGGAAACAACACTCAACGAAGTTATTCCATCATCCCGTGTTTTATATTTGTTGAGCTTGTCA
  TCATGGCTGGGACAGTGCTGCTTGCCTACTACTTCGAATGCACTGACACTTTTCAGGTGCATATCCAAGG
  ATTCTTCTGTCAGGACGGAGACTTAATGAAGCCTTACCCAGGGACAGAGGAAGAAAGCTTCATCACCCCT
  CTGGTGCTCTATTGTGTGCTGGCTGCCACCCCAACTGCTATTATTTTTATTGGTGAGATATCCATGTATT
  TCATAAAATCAACAAGAGAATCCCTGATTGCTCAGGAGAAAACAATTCTGACCGGAGAATGCTGTTACCT
  GAACCCCTTACTTCGAAGGATCATAAGATTCACAGGGGTGTTTGCATTTGGACTTTTTGCTACTGACATT
  TTTGTAAACGCCGGACAAGTGGTCACTGGGCACTTAACGCCATACTTCCTGACTGTGTGCAAGCCAAACT
  ACACCAGTGCAGACTGCCAAGCGCACCACCAGTTTATAAACAATGGGAACATTTGTACTGGGGACCTGGA
  AGTGATAGAAAAGGCTCGGAGATCCTTTCCCTCCAAACACGCTGCTCTGAGCATTTACTCCGCCTTATAT
  GCCACGATGTATATTACAAGCACAATCAAGACGAAGAGCAGTCGACTGGCCAAGCCGGTGCTGTGCCTCG
  GAACTCTCTGCACAGCCTTCCTGACAGGCCTCAACCGGGTCTCTGAGTATCGGAACCACTGCTCGGACGT
  GATTGCTGGTTTCATCCTGGGCACTGCAGTGGCCCTGTTTCTGGGAATGTGTGTGGTTCATAACTTTAAA
  GGAACGCAAGGATCTCCTTCCAAACCCAAGCCTGAGGATCCCCGTGGAGTACCCCTAATGGCTTTCCCAA
  GGATAGAAAGCCCTCTGGAAACCTTAAGTGCACAGAATCACTCTGCGTCCATGACCGAAGTTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222205 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVGNNTQRSYSIIPCFIFVELVIMAGTVLLAYYFECTDTFQVHIQGFFCQDGDLMKPYPGTEEESFITP
  LVLYCVLAATPTAIIFIGEISMYFIKSTRESLIAQEKTILTGECCYLNPLLRRIIRFTGVFAFGLFATDI
  FVNAGQVVTGHLTPYFLTVCKPNYTSADCQAHHQFINNGNICTGDLEVIEKARRSFPSKHAALSIYSALY
  ATMYITSTIKTKSSRLAKPVLCLGTLCTAFLTGLNRVSEYRNHCSDVIAGFILGTAVALFLGMCVVHNFK
  GTQGSPSKPKPEDPRGVPLMAFPRIESPLETLSAQNHSASMTEVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207299
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207299.1, NP_997182.1
• RefSeq Size: 2461 bp
• RefSeq ORF: 978 bp
• Locus ID: 54886
• Protein Families: Phosphatase, Transmembrane
• MW: 35.8 kDa
• Gene Summary: This gene encodes a member of the plasticity-related gene (PRG) family. Members of the PRG family mediate lipid phosphate phosphatase activity in neurons and are known to be involved in neuronal plasticity. The protein encoded by this gene does not perform its function through enzymatic phospholipid degradation. This gene is strongly expressed in brain. It shows dynamic expression regulation during brain development and neuronal excitation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]