H3FJ (HIST1H3J) (NM_003535) Human Tagged ORF Clone

CAT#: RC222226

HIST1H3J (Myc-DDK-tagged)-Human histone cluster 1, H3j (HIST1H3J)

Product Images

Western blot validation of overexpression lysate (Cat# LY418623) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222226 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HIST1H3J
• Synonyms: H3/j; H3FJ
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222226 representing NM_003535
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGACGAAGCAGACAGCTCGCAAGTCTACCGGCGGCAAGGCACCGCGGAAGCAGCTGGCCACCA
  AGGCAGCGCGCAAAAGCGCTCCAGCGACTGGCGGTGTGAAGAAGCCCCACCGCTACAGGCCAGGCACCGT
  GGCCTTGCGTGAGATCCGCCGTTATCAGAAGTCGACTGAGCTGCTCATCCGCAAACTGCCATTTCAGCGC
  CTGGTGCGAGAAATCGCGCAGGATTTCAAAACCGACCTTCGTTTCCAGAGCTCGGCGGTGATGGCGCTGC
  AAGAGGCGTGCGAGGCCTATCTGGTGGGTCTCTTTGAAGACACCAACCTCTGTGCTATTCACGCCAAGCG
  TGTCACTATTATGCCTAAGGACATCCAGCTTGCGCGTCGTATCCGTGGCGAGCGAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222226 representing NM_003535
  Red=Cloning site Green=Tags(s)
  
  MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQR
  LVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003535
• ORF Size: 408 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003535.1, NM_003535.2, NP_003526.1
• RefSeq Size: 478
• RefSeq ORF: 411
• Locus ID: 8356
• Protein Pathways: Systemic lupus erythematosus
• MW: 15.2 kDa
• Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]