Hemopexin (HPX) (NM_000613) Human Tagged ORF Clone

CAT#: RC222271

HPX (Myc-DDK-tagged)-Human hemopexin (HPX)

Product Images

Western blot validation of overexpression lysate (Cat# LY400205) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222271 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HPX protein (Cat# TP322271). The protein was produced from HEK293T cells transfected with HPX cDNA clone (Cat# RC222271) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HPX
• Synonyms: HX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222271 representing NM_000613
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAGGGTACTGGGAGCACCCGTTGCACTGGGGTTGTGGAGCCTATGCTGGTCTCTGGCCATTGCCA
  CCCCTCTTCCTCCGACTAGTGCCCATGGGAATGTTGCTGAAGGCGAGACCAAGCCAGACCCAGACGTGAC
  TGAACGCTGCTCAGATGGCTGGAGCTTTGATGCTACCACCCTGGATGACAATGGAACCATGCTGTTTTTT
  AAAGGGGAGTTTGTGTGGAAGAGTCACAAATGGGACCGGGAGTTAATCTCAGAGAGATGGAAGAATTTCC
  CCAGCCCTGTGGATGCTGCATTCCGTCAAGGTCACAACAGTGTCTTTCTGATCAAGGGGGACAAAGTCTG
  GGTATACCCTCCTGAAAAGAAGGAGAAAGGATACCCAAAGTTGCTCCAAGATGAATTTCCTGGAATCCCA
  TCCCCACTGGATGCAGCTGTGGAATGTCACCGTGGAGAATGTCAAGCTGAAGGCGTCCTCTTCTTCCAAG
  GTGACCGCGAGTGGTTCTGGGACTTGGCTACGGGAACCATGAAGGAGCGTTCCTGGCCAGCTGTTGGGAA
  CTGCTCCTCTGCCCTGAGATGGCTGGGCCGCTACTACTGCTTCCAGGGTAACCAATTCCTGCGCTTCGAC
  CCTGTCAGGGGAGAGGTGCCTCCCAGGTACCCGCGGGATGTCCGAGACTACTTCATGCCCTGCCCTGGCA
  GAGGCCATGGACACAGGAATGGGACTGGCCATGGGAACAGTACCCACCATGGCCCTGAGTATATGCGCTG
  TAGCCCACATCTAGTCTTGTCTGCACTGACGTCTGACAACCATGGTGCCACCTATGCCTTCAGTGGGACC
  CACTACTGGCGTCTGGACACCAGCCGGGATGGCTGGCATAGCTGGCCCATTGCTCATCAGTGGCCCCAGG
  GTCCTTCAGCAGTGGATGCTGCCTTTTCCTGGGAAGAAAAACTCTATCTGGTCCAGGGCACCCAGGTATA
  TGTCTTCCTGACAAAGGGAGGCTATACCCTAGTAAGCGGTTATCCGAAGCGGCTGGAGAAGGAAGTCGGG
  ACCCCTCATGGGATTATCCTGGACTCTGTGGATGCGGCCTTTATCTGCCCTGGGTCTTCTCGGCTCCATA
  TCATGGCAGGACGGCGGCTGTGGTGGCTGGACCTGAAGTCAGGAGCCCAAGCCACGTGGACAGAGCTTCC
  TTGGCCCCATGAGAAGGTAGACGGAGCCTTGTGTATGGAAAAGTCCCTTGGCCCTAACTCATGTTCCGCC
  AATGGTCCCGGCTTGTACCTCATCCATGGTCCCAATTTGTACTGCTACAGTGATGTGGAGAAACTGAATG
  CAGCCAAGGCCCTTCCGCAACCCCAGAATGTGACCAGTCTCCTGGGCTGCACTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222271 representing NM_000613
  Red=Cloning site Green=Tags(s)
  
  MARVLGAPVALGLWSLCWSLAIATPLPPTSAHGNVAEGETKPDPDVTERCSDGWSFDATTLDDNGTMLFF
  KGEFVWKSHKWDRELISERWKNFPSPVDAAFRQGHNSVFLIKGDKVWVYPPEKKEKGYPKLLQDEFPGIP
  SPLDAAVECHRGECQAEGVLFFQGDREWFWDLATGTMKERSWPAVGNCSSALRWLGRYYCFQGNQFLRFD
  PVRGEVPPRYPRDVRDYFMPCPGRGHGHRNGTGHGNSTHHGPEYMRCSPHLVLSALTSDNHGATYAFSGT
  HYWRLDTSRDGWHSWPIAHQWPQGPSAVDAAFSWEEKLYLVQGTQVYVFLTKGGYTLVSGYPKRLEKEVG
  TPHGIILDSVDAAFICPGSSRLHIMAGRRLWWLDLKSGAQATWTELPWPHEKVDGALCMEKSLGPNSCSA
  NGPGLYLIHGPNLYCYSDVEKLNAAKALPQPQNVTSLLGCTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000613
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000613.1, NM_000613.2, NP_000604.1
• RefSeq Size: 1389 bp
• RefSeq ORF: 1389 bp
• Locus ID: 3263
• Cytogenetics: 11p15.4
• Domains: hemopexin
• Protein Families: Secreted Protein
• MW: 51.68 kDa
• Gene Summary: 'This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq, Apr 2009]'