Drebrin (DBN1) (NM_080881) Human Tagged ORF Clone

CAT#: RC222368

DBN1 (Myc-DDK-tagged)-Human drebrin 1 (DBN1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBN1
• Synonyms: D0S117E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222368 representing NM_080881
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGCATCCATGGCATGGTACTGCAGCACTGGCCAGCAGCCAGGCCTGGAGGGATGGACGCGAGA
  GACAAGCTCTCGTGTCCTGCAGGGCTCTGTACACATATGAAGATGGCTCCGATGACCTCAAGCTTGCAGC
  ATCAGGAGAAGGGGGCTTGCAGGAGCTTTCGGGACACTTTGAGAACCAGAAGGTGATGTACGGCTTCTGC
  AGTGTCAAGGACTCCCAAGCTGCTCTGCCAAAATACGTGCTCATCAACTGGGTGGGCGAAGATGTGCCTG
  ATGCCCGCAAGTGCGCTTGTGCCAGCCACGTGGCTAAGGTGGCGGAGTTCTTCCAGGGTGTCGACGTGAT
  CGTGAACGCCAGCAGCGTGGAAGACATAGACGCGGGTGCCATCGGGCAGCGGCTCTCTAACGGGCTGGCG
  CGACTCTCCAGCCCTGTGCTGCACCGACTGCGGCTGCGAGAGGATGAGAACGCAGAGCCCGTGGGCACCA
  CCTACCAGAAGACGGATGCAGCTGTGGAAATGAAGCGGATTAACCGAGAGCAGTTCTGGGAGCAGGCCAA
  GAAGGAAGAAGAGCTGCGGAAGGAGGAGGAGCGGAAGAAGGCCCTGGATGAGAGGCTCAGGTTCGAGCAG
  GAGCGGATGGAGCAGGAGCGGCAGGAGCAAGAGGAGCGCGAGCGGCGCTACCGGGAGCGGGAGCAGCAGA
  TCGAGGAGCACAGGAGGAAACAGCAGACTTTAGAAGCGGAAGAGGCCAAGAGGCGGTTGAAGGAGCAGTC
  TATCTTTGGTGACCATCGGGATGAGGAGGAAGAGACCCACATGAAGAAGTCAGAGTCGGAGGTGGAGGAG
  GCAGCAGCTATTATTGCCCAGCGGCCTGACAACCCAAGGGAGTTCTTCAAGCAGCAGGAAAGAGTCGCAT
  CGGCCTCTGCGGGCAGCTGTGATGTACCCTCGCCCTTCAACCATCGACCAGGCAGCCACCTGGACAGCCA
  CCGGAGGATGGCGCCCACTCCCATCCCCACGCGGAGCCCGTCTGACTCCAGCACCGCCTCCACCCCTGTC
  GCTGAGCAGATAGAGCGGGCCCTGGATGAGGTCACCTCCTCGCAGCCTCCACCACTGCCACCGCCACCCC
  CACCAGCCCAAGAGACCCAGGAGCCCAGCCCCATCCTAGACAGTGAGGAGACCAGAGCAGCAGCCCCTCA
  GGCCTGGGCCGGCCCCATGGAGGAGCCCCCTCAGGCACAGGCGCCTCCCCGGGGGCCAGGCAGCCCTGCA
  GAGGACTTGATGTTCATGGAGTCTGCAGAGCAGGCTGTCCTGGCTGCTCCCGTGGAGCCTGCCACAGCTG
  ACGCCACGGAGATCCACGATGCAGCTGACACCATTGAAACTGACACTGCCACTGCTGACACCACTGTTGC
  CAACAACGTACCCCCCGCCGCCACCAGCCTCATTGACCTATGGCCTGGCAACGGGGAAGGGGCCTCCACA
  CTCCAGGGTGAGCCCAGGGCCCCCACGCCACCCTCGGGTACTGAGGTCACCCTGGCAGAGGTGCCCCTGC
  TGGATGAGGTGGCTCCGGAGCCACTGCTGCCAGCAGGCGAAGGCTGTGCCACCCTTCTCAACTTTGATGA
  GCTGCCTGAGCCGCCAGCCACCTTCTGTGACCCAGAGGAAGTGGAAGGGGAGCCCCTGGCTGCCCCCCAG
  ACCCCAACTCTGCCCTCAGCCCTTGAGGAGCTGGAGCAAGAGCAGGAGCCGGAGCCCCACCTGCTAACCA
  ATGGCGAGACCACCCAGAAGGAGGGGACCCAGGCCAGTGAGGGGTACTTCAGTCAATCACAGGAGGAGGA
  GTTTGCCCAATCGGAAGAGCTCTGTGCCAAGGCTCCGCCTCCTGTGTTCTACAACAAGCCTCCAGAGATC
  GACATCACATGCTGGGATGCAGACCCAGTTCCAGAAGAGGAGGAGGGCTTCGAGGGTGGTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222368 representing NM_080881
  Red=Cloning site Green=Tags(s)
  
  MAGHPWHGTAALASSQAWRDGRERQALVSCRALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFC
  SVKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLA
  RLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDERLRFEQ
  ERMEQERQEQEERERRYREREQQIEEHRRKQQTLEAEEAKRRLKEQSIFGDHRDEEEETHMKKSESEVEE
  AAAIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPV
  AEQIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPA
  EDLMFMESAEQAVLAAPVEPATADATEIHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGAST
  LQGEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGEPLAAPQ
  TPTLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPPPVFYNKPPEI
  DITCWDADPVPEEEEGFEGGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080881
• ORF Size: 1953 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080881.1, NM_080881.2, NP_543157.1
• RefSeq Size: 3383 bp
• RefSeq ORF: 1956 bp
• Locus ID: 1627
• Cytogenetics: 5q35.3
• MW: 71.4 kDa
• Gene Summary: 'The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'