MTMR7 (NM_004686) Human Tagged ORF Clone

CAT#: RC222373

MTMR7 (Myc-DDK-tagged)-Human myotubularin related protein 7 (MTMR7)

Product Images

Western blot validation of overexpression lysate (Cat# LY417811) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222373 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified MTMR7 protein (Cat# TP322373). The protein was produced from HEK293T cells transfected with MTMR7 cDNA clone (Cat# RC222373) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTMR7
• Synonyms: DKFZp781E194; MGC163449; MGC163451
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222373 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCACATCCGCACGCCCAAGGTTGAAAATGTCCGCTTGGTAGATCGAGTGTCTCCTAAAAAAGCAG
  CTCTAGGTACTTTGTATTTGACGGCTACCCATGTCATATTCGTGGAAAATTCACCTGACGCAAGAAAAGA
  AACATGGATTCTTCACAGTCAGATTTCCACCATTGAGAAACAGGCAACAACCGCTACCGGATGCCCTCTG
  CTGATTCGCTGCAAGAACTTTCAGATAATACAGCTCATCATACCTCAGGAAAGAGATTGCCACGACGTGT
  ACATCTCCCTGATACGCCTTGCAAGGCCAGTGAAATATGAGGAGTTATACTGCTTTTCATTCAACCCCAT
  GCTGGATAAAGAAGAAAGAGAGCAAGGCTGGGTGCTGATCGATCTTAGTGAAGAATACACGCGGATGGGC
  CTCCCTAATCATTACTGGCAGCTCAGCGATGTGAATAGAGACTATAGAGTCTGTGACTCTTATCCTACTG
  AACTGTACGTTCCCAAATCGGCCACGGCACACATCATAGTGGGGAGTTCCAAATTCCGGAGTAGACGGCG
  ATTTCCTGTCCTTTCTTACTATTATAAAGATAACCACGCCTCCATCTGCCGGAGCAGCCAGCCCCTGTCC
  GGCTTCAGTGCCCGGTGCCTAGAGGACGAGCAGATGCTCCAGGCCATTAGGAAAGCCAATCCAGGAAGTG
  ACTTCGTTTATGTCGTTGACACCCGGCCTAAACTTAATGCAATGGCAAATCGTGCTGCAGGGAAAGGCTA
  TGAGAATGAAGACAATTATTCCAATATCAAGTTTCAGTTTATCGGGATAGAGAACATCCATGTCATGAGG
  AACAGTCTGCAGAAAATGCTGGAAGTGTGTGAACTTAAATCTCCCTCCATGAGTGATTTCCTGTGGGGTC
  TGGAGAACTCTGGCTGGTTAAGGCACATTAAAGCCATAATGGATGCAGGAATCTTCATTGCAAAGGCAGT
  GTCAGAGGAAGGGGCAAGTGTGCTTGTTCACTGTTCTGATGGCTGGGACAGGACCGCTCAGGTGTGCTCG
  GTGGCAAGCCTGCTGCTGGACCCTCACTACCGGACTCTGAAGGGCTTCATGGTATTAATTGAAAAGGACT
  GGATTTCCTTTGGTCATAAGTTTAATCACCGATATGGCAATCTAGATGGTGACCCAAAAGAAATCTCTCC
  AGTTATTGACCAGTTCATTGAGTGTGTTTGGCAGTTAATGGAACAATTTCCCTGTGCCTTTGAGTTCAAT
  GAGAGGTTTTTGATTCACATTCAACATCACATTTATTCCTGCCAGTTTGGAAACTTCCTATGTAACAGCC
  AAAAGGAGAGACGAGAACTCAAGATTCAAGAAAGAACATACTCATTATGGGCTCACCTGTGGAAGAATCG
  GGCCGACTACCTGAATCCTCTGTTTAGAGCTGATCACAGCCAGACTCAGGGAACCCTTCATCTCCCTACA
  ACACCATGTAACTTCATGTACAAGTTTTGGAGTGGAATGTATAACCGCTTTGAAAAGGGGATGCAGCCCC
  GACAGTCAGTTACAGATTACCTAATGGCAGTGAAGGAAGAAACTCAGCAGCTAGAGGAAGAACTAGAGGC
  CCTGGAAGAAAGGCTGGAAAAAATTCAAAAGGTCCAGTTAAATTGCACTAAGGTGAAGAGTAAGCAAAGT
  GAGCCCAGCAAGCACTCAGGGTTTTCTACCTCAGACAACAGCATAGCCAACACTCCCCAGGATTACAGTG
  GGAATATGAAATCATTTCCATCCCGGAGCCCTTCACAAGGCGATGAAGATTCTGCTCTGATTCTAACCCA
  AGACAATCTGAAAAGTTCAGATCCAGATCTGTCAGCCAACAGTGACCAAGAGTCCGGGGTGGAGGATTTG
  AGCTGTCGGTCTCCAAGTGGTGGTGAGCATGCACCGAGTGAAGATAGTGGCAAGGACCGGGATTCTGATG
  AAGCCGTGTTTCTCACTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222373 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEHIRTPKVENVRLVDRVSPKKAALGTLYLTATHVIFVENSPDARKETWILHSQISTIEKQATTATGCPL
  LIRCKNFQIIQLIIPQERDCHDVYISLIRLARPVKYEELYCFSFNPMLDKEEREQGWVLIDLSEEYTRMG
  LPNHYWQLSDVNRDYRVCDSYPTELYVPKSATAHIIVGSSKFRSRRRFPVLSYYYKDNHASICRSSQPLS
  GFSARCLEDEQMLQAIRKANPGSDFVYVVDTRPKLNAMANRAAGKGYENEDNYSNIKFQFIGIENIHVMR
  NSLQKMLEVCELKSPSMSDFLWGLENSGWLRHIKAIMDAGIFIAKAVSEEGASVLVHCSDGWDRTAQVCS
  VASLLLDPHYRTLKGFMVLIEKDWISFGHKFNHRYGNLDGDPKEISPVIDQFIECVWQLMEQFPCAFEFN
  ERFLIHIQHHIYSCQFGNFLCNSQKERRELKIQERTYSLWAHLWKNRADYLNPLFRADHSQTQGTLHLPT
  TPCNFMYKFWSGMYNRFEKGMQPRQSVTDYLMAVKEETQQLEEELEALEERLEKIQKVQLNCTKVKSKQS
  EPSKHSGFSTSDNSIANTPQDYSGNMKSFPSRSPSQGDEDSALILTQDNLKSSDPDLSANSDQESGVEDL
  SCRSPSGGEHAPSEDSGKDRDSDEAVFLTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004686
• ORF Size: 1980 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004686.2, NM_004686.3, NM_004686.4, NP_004677.2
• RefSeq Size: 5302 bp
• RefSeq ORF: 1983 bp
• Locus ID: 9108
• Protein Families: Phosphatase
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways, Riboflavin metabolism, Thiamine metabolism
• MW: 75.8 kDa
• Gene Summary: This gene encodes a member of the myotubularin family of tyrosine/dual-specificity phosphatases. The encoded protein is characterized by four distinct domains that are conserved among all members of the myotubularin family: the glucosyltransferase, Rab-like GTPase activator and myotubularins domain, the Rac-induced recruitment domain, the protein tyrosine phosphatases and dual-specificity phosphatases domain and the suppressor of variegation 3-9, enhancer-of-zeste, and trithorax interaction domain. This protein dephosphorylates the target substrates phosphatidylinositol 3-phosphate and inositol 1,3-bisphosphate. A pseudogene of this gene is found on chromosome 5. [provided by RefSeq, Mar 2009]