RALGDS (NM_001042368) Human Tagged ORF Clone
CAT#: RC222398
RALGDS (Myc-DDK-tagged)-Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2
"NM_001042368" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RALGDS |
Synonyms | RalGEF; RGDS; RGF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222398 representing NM_001042368
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGTAGATTGCCAGAGCTCCACGCAGGAGATCGGTGAGGAGCTGATCAACGGAGTCATCTACTCCA TCTCCCTGCGCAAGGTGCAGCTGCACCACGGAGGCAACAAGGGGCAGCGCTGGCTCGGGTATGAGAATGA GTCGGCCCTGAACCTTTATGAGACTTGCAAGGTGCGGACCGTGAAGGCTGGCACGCTGGAGAAGCTGGTG GAGCACCTGGTGCCAGCCTTCCAGGGCAGCGACCTCTCCTACGTCACCATCTTCCTGTGTACCTATAGAG CCTTCACCACCACCCAACAGGTCCTGGACCTGCTGTTCAAAAGGTACGGTAGATGTGACGCCCTCACGGC CTCCTCTAGATACGGCTGCATCCTCCCCTATTCCGACGAGGATGGTGGACCCCAGGACCAACTTAAAAAT GCCATCTCCTCCATCCTGGGCACCTGGCTGGACCAGTACTCGGAGGATTTCTGTCAACCTCCGGACTTTC CCTGCCTCAAGCAGCTGGTGGCCTACGTGCAGCTCAACATGCCAGGCTCAGACCTGGAGCGCCGTGCCCA CCTTCTCCTGGCCCAGCTGGAGCACTCGGAACCCATTGAGGCAGAGCCTGAGGCTCTGTCACCAGTGCCA GCTCTAAAACCAACTCCAGAGCTCGAGCTAGCTCTAACACCAGCTCGAGCACCCAGCCCAGTGCCGGCTC CAGCCCCGGAGCCAGAGCCAGCTCCAACACCAGCTCCAGGTTCAGAGCTAGAAGTAGCTCCAGCACCAGC TCCGGAGCTCCAGCAGGCTCCAGAGCCAGCTGTGGGACTAGAATCGGCTCCAGCGCCAGCTCTGGAACTA GAGCCAGCTCCAGAACAGGATCCAGCTCCCTCACAAACTCTAGAGCTGGAGCCAGCTCCAGCACCAGTTC CATCATTACAGCCTTCCTGGCCTTCACCTGTGGTTGCAGAGAACGGGCTGAGTGAGGAGAAGCCTCACCT CTTGGTGTTCCCTCCAGATCTGGTGGCAGAGCAGTTTACACTGATGGATGCGGAACTGTTCAAGAAGGTG GTGCCCTACCACTGCCTGGGCTCCATCTGGTCCCAGCGGGACAAGAAGGGCAAGGAGCACCTGGCGCCCA CCATCCGCGCCACTGTCACCCAGTTCAACAGTGTGGCCAACTGTGTCATCACCACCTGCCTCGGGAACCG AAGCACGAAAGCCCCAGACAGGGCCAGGGTGGTGGAGCACTGGATCGAGGTGGCCAGGGAGTGCCGGATC CTCAAGAACTTCTCGTCACTGTATGCCATCCTCTCTGCCCTGCAGAGCAACTCCATCCACCGTCTGAAGA AGACGTGGGAAGACGTTTCCAGGGACAGTTTCCGGATCTTTCAGAAGCTGTCAGAGATCTTCTCAGATGA GAACAACTACTCATTGAGCCGGGAGCTGCTCATCAAGGAGGGCACCTCCAAGTTTGCCACCCTGGAGATG AACCCCAAGAGAGCCCAGAAACGGCCGAAGGAGACGGGCATCATCCAGGGCACCGTTCCCTACCTGGGCA CGTTCCTCACCGACCTGGTGATGCTGGACACTGCCATGAAGGACTATCTGTATGGCAGACTCATCAACTT TGAGAAGAGGAGGAAGGAGTTCGAGGTGATCGCCCAGATCAAGCTGCTGCAGTCGGCCTGCAACAACTAC AGCATCGCGCCAGATGAGCAATTTGGGGCCTGGTTCCGGGCCGTGGAGCGGCTCAGCGAGACTGAGAGCT ACAACCTGTCGTGCGAGCTGGAGCCCCCATCCGAGTCAGCCAGCAACACCCTCAGGACCAAGAAGAACAC AGCCATTGTCAAGCGCTGGAGCGACCGCCAGGCCCCCAGCACTGAGCTCAGTACCAGTGGCAGCTCCCAC TCCAAGTCCTGTGACCAGCTCAGGTGTGGCCCCTACCTCAGCAGCGGGGACATCGCTGACGCGCTCAGCG TGCACTCGGCCGGCTCCTCTAGCTCCGACGTGGAGGAGATCAACATCAGCTTCGTCCCGGAGTCTCCTGA TGGCCAGGAAAAGAAGTTCTGGGAATCAGCCTCACAGTCATCCCCGGAGACCTCCGGCATCAGCTCAGCC TCCAGCAGCACCTCGTCCTCCTCAGCCTCCACCACGCCCGTGGCTGCCACACGCACCCACAAGCGCTCTG TCTCAGGGCTCTGCAACTCCAGCTCCGCGCTGCCGCTCTACAACCAGCAGGTGGGCGACTGCTGTATCAT CCGCGTCAGCCTGGACGTGGACAATGGCAACATGTACAAGAGCATCCTGGTGACCAGCCAAGATAAGGCT CCGGCTGTAATCCGCAAGGCCATGGACAAACACAACCTGGAGGAGGAGGAGCCGGAGGACTATGAGCTGC TGCAGATTCTCTCAGATGACCGGAAGCTGAAGATCCCTGAAAACGCCAACGTCTTCTATGCCATGAACTC TACCGCCAACTATGACTTTGTCCTCAAGAAGCGGACCTTCACCAAGGGAGTGAAGGTCAAGCACGGAGCC AGCTCCACCCTCCCTCGCATGAAGCAGAAAGGACTCAAGATTGCCAAGGGCATCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222398 representing NM_001042368
Red=Cloning site Green=Tags(s) MMVDCQSSTQEIGEELINGVIYSISLRKVQLHHGGNKGQRWLGYENESALNLYETCKVRTVKAGTLEKLV EHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLLFKRYGRCDALTASSRYGCILPYSDEDGGPQDQLKN AISSILGTWLDQYSEDFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEHSEPIEAEPEALSPVP ALKPTPELELALTPARAPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELQQAPEPAVGLESAPAPALEL EPAPEQDPAPSQTLELEPAPAPVPSLQPSWPSPVVAENGLSEEKPHLLVFPPDLVAEQFTLMDAELFKKV VPYHCLGSIWSQRDKKGKEHLAPTIRATVTQFNSVANCVITTCLGNRSTKAPDRARVVEHWIEVARECRI LKNFSSLYAILSALQSNSIHRLKKTWEDVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEM NPKRAQKRPKETGIIQGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNY SIAPDEQFGAWFRAVERLSETESYNLSCELEPPSESASNTLRTKKNTAIVKRWSDRQAPSTELSTSGSSH SKSCDQLRCGPYLSSGDIADALSVHSAGSSSSDVEEINISFVPESPDGQEKKFWESASQSSPETSGISSA SSSTSSSSASTTPVAATRTHKRSVSGLCNSSSALPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKA PAVIRKAMDKHNLEEEEPEDYELLQILSDDRKLKIPENANVFYAMNSTANYDFVLKKRTFTKGVKVKHGA SSTLPRMKQKGLKIAKGIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001042368 |
ORF Size | 2577 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001042368.1, NM_001042368.2, NP_001035827.1 |
RefSeq Size | 3668 bp |
RefSeq ORF | 2580 bp |
Locus ID | 5900 |
Cytogenetics | 9q34.13-q34.2 |
Protein Pathways | Colorectal cancer, Pancreatic cancer, Pathways in cancer |
MW | 94.5 kDa |
Gene Summary | 'Guanine nucleotide dissociation stimulators (GDSs, or exchange factors), such as RALGDS, are effectors of Ras-related GTPases (see MIM 190020) that participate in signaling for a variety of cellular processes.[supplied by OMIM, Nov 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC311316 | RALGDS (untagged)-Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2 |
USD 1,750.00 |
|
RG222398 | RALGDS (GFP-tagged) - Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2 |
USD 1,060.00 |
|
RC222398L3 | Lenti ORF clone of Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2, Myc-DDK-tagged |
USD 1,160.00 |
|
RC222398L4 | Lenti ORF clone of Human ral guanine nucleotide dissociation stimulator (RALGDS), transcript variant 2, mGFP tagged |
USD 1,160.00 |
{0} Product Review(s)
Be the first one to submit a review