OGA (NM_012215) Human Tagged ORF Clone

CAT#: RC222411

MGEA5 (Myc-DDK-tagged)-Human meningioma expressed antigen 5 (hyaluronidase) (MGEA5), transcript variant 1

Product Images

Coomassie blue staining of purified OGA protein (Cat# TP322411). The protein was produced from HEK293T cells transfected with OGA cDNA clone (Cat# RC222411) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OGA
• Synonyms: MEA5; MGEA5; NCOAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222411 representing NM_012215
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGAAGGAGAGTCAAGCGACGTTGGAGGAGCGGGAGAGCGAGCTCAGCTCCAACCCTGCCGCCT
  CTGCGGGGGCATCGCTGGAGCCGCCGGCAGCTCCGGCACCCGGAGAAGACAACCCCGCCGGGGCTGGGGG
  AGCGGCGGTGGCCGGGGCTGCAGGAGGGGCTCGGCGGTTCCTCTGCGGTGTGGTGGAAGGATTTTATGGA
  AGACCTTGGGTTATGGAACAGAGAAAAGAACTCTTTAGAAGGCTCCAGAAATGGGAATTAAATACATACT
  TGTATGCCCCAAAAGATGACTACAAACATAGGATGTTTTGGCGAGAGATGTATTCAGTGGAGGAAGCTGA
  GCAACTTATGACTCTCATCTCTGCTGCACGAGAATATGAGATAGAGTTCATCTATGCGATCTCACCTGGA
  TTGGATATCACTTTTTCTAACCCCAAGGAAGTATCCACATTGAAACGTAAATTGGACCAGGTTTCTCAGT
  TTGGGTGCAGATCATTTGCTTTGCTTTTTGATGATATAGACCATAATATGTGTGCAGCAGACAAAGAGGT
  ATTCAGTTCTTTTGCTCATGCCCAAGTCTCCATCACAAATGAAATCTATCAGTACCTAGGAGAGCCAGAA
  ACTTTCCTCTTCTGTCCCACAGAATACTGTGGCACTTTCTGTTATCCAAATGTGTCTCAGTCTCCATATT
  TAAGGACTGTGGGTGAAAAGCTTCTACCTGGAATTGAAGTGCTTTGGACAGGTCCCAAAGTTGTTTCTAA
  AGAAATTCCAGTAGAGTCCATCGAAGAGGTTTCTAAGATTATTAAGAGAGCTCCAGTAATCTGGGATAAC
  ATTCATGCTAATGATTATGATCAGAAGAGACTGTTTCTGGGCCCGTACAAAGGAAGATCCACAGAACTCA
  TCCCACGGTTAAAAGGAGTCCTCACTAATCCAAATTGTGAATTTGAAGCCAACTACGTTGTTATCCACAC
  CCTTGCCACCTGGTACAAATCAAACATGAATGGAGTGAGAAAAGATGTAGTGATGACTGACAGTGAAGAT
  AGTACTGTGTCCATCCAGATAAAATTAGAAAATGAAGGCAGTGATGAAGATATTGAAACTGATGTACTCT
  ATAGTCCACAGATGGCTCTAAAGCTAGCATTAACAGAATGGTTGCAAGAGTTTGGTGTGCCTCATCAATA
  CAGCAGTAGGCAAGTTGCACACAGTGGAGCTAAAGCAAGTGTAGTTGATGGGACTCCTTTAGTTGCAGCA
  CCCTCTTTAAATGCCACAACCGTAGTAACAACAGTTTATCAGGAGCCCATTATGAGCCAGGGAGCAGCCT
  TGAGTGGTGAGCCTACTACTCTGACCAAGGAAGAAGAAAAGAAACAGCCTGATGAAGAACCCATGGACAT
  GGTGGTGGAAAAACAAGAAGAAACGGACCACAAGAATGACAATCAAATACTGAGTGAAATTGTTGAAGCG
  AAAATGGCAGAGGAATTGAAACCAATGGACACTGATAAAGAGAGCATAGCTGAATCAAAATCCCCAGAGA
  TGTCCATGCAAGAAGATTGTATTAGTGACATTGCCCCCATGCAAACTGATGAACAGACAAACAAGGAGCA
  GTTTGTGCCAGGTCCAAATGAAAAGCCTTTGTACACTGCGGAACCAGTGACCCTGGAGGATTTGCAGTTA
  CTTGCTGATCTATTCTACCTTCCTTACGAGCATGGACCCAAAGGAGCACAGATGTTACGGGAATTTCAAT
  GGCTTCGAGCAAATAGTAGTGTTGTCAGTGTCAATTGCAAAGGAAAAGACTCTGAAAAAATTGAAGAATG
  GCGGTCACGAGCAGCCAAGTTTGAAGAGATGTGTGGACTAGTGATGGGAATGTTCACTCGGCTCTCCAAT
  TGTGCCAACAGGACAATTCTTTATGACATGTACTCCTATGTTTGGGATATCAAGAGTATAATGTCTATGG
  TGAAGTCTTTTGTACAGTGGTTAGGGTGTCGTAGTCATTCTTCAGCACAATTCTTAATTGGAGACCAAGA
  ACCCTGGGCCTTTAGAGGTGGTCTAGCAGGAGAGTTCCAGCGTTTGCTGCCAATTGATGGGGCAAATGAT
  CTCTTTTTTCAGCCACCTCCACTGACTCCTACCTCCAAAGTTTATACTATCAGACCTTATTTTCCTAAGG
  ATGAGGCATCCGTGTACAAGATTTGCAGAGAAATGTATGACGATGGAGTGGGTTTACCCTTTCAAAGTCA
  GCCTGATCTTATTGGAGACAAGTTAGTAGGAGGGCTGCTTTCCCTCAGCCTGGATTACTGCTTTGTCCTA
  GAAGATGAAGATGGCATATGTGGTTATGCCTTGGGCACTGTAGATGTGACCCCCTTTATTAAAAAATGTA
  AAATTTCCTGGATCCCCTTCATGCAGGAGAAGTATACCAAGCCAAATGGTGACAAGGAACTCTCTGAGGC
  TGAGAAAATAATGTTGAGTTTCCATGAAGAACAGGAAGTACTGCCAGAAACTTTCCTTGCTAATTTCCCT
  TCTCTGATAAAGATGGACATTCACAAAAAAGTAACTGACCCAAGTGTGGCCAAAAGCATGATGGCTTGCC
  TCCTGTCTTCACTGAAGGCTAATGGCTCCCGGGGAGCTTTCTGTGAAGTGAGACCAGATGATAAAAGAAT
  TCTGGAATTTTACAGCAAGTTAGGATGTTTTGAAATTGCAAAAATGGAAGGATTTCCAAAGGATGTGGTT
  ATACTTGGTCGGAGCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222411 representing NM_012215
  Red=Cloning site Green=Tags(s)
  
  MVQKESQATLEERESELSSNPAASAGASLEPPAAPAPGEDNPAGAGGAAVAGAAGGARRFLCGVVEGFYG
  RPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPG
  LDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPE
  TFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDN
  IHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVVIHTLATWYKSNMNGVRKDVVMTDSED
  STVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKASVVDGTPLVAA
  PSLNATTVVTTVYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEA
  KMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQL
  LADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSN
  CANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGAND
  LFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGLPFQSQPDLIGDKLVGGLLSLSLDYCFVL
  EDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFP
  SLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVV
  ILGRSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012215
• ORF Size: 2748 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012215.1, NM_012215.2, NM_012215.3, NP_036347.1
• RefSeq Size: 5220
• RefSeq ORF: 2751
• Locus ID: 10724
• MW: 102.9 kDa
• Gene Summary: The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]). [supplied by OMIM, Mar 2008]