CD89 (FCAR) (NM_133269) Human Tagged ORF Clone

CAT#: RC222470

FCAR (Myc-DDK-tagged)-Human Fc fragment of IgA, receptor for (FCAR), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY408857) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222470 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FCAR
• Synonyms: CD89; CTB-61M7.2; FcalphaRI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222470 representing NM_133269
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCAAACAGACCACCCTCCTGTGTCTTGTGCTCTGTCTGGGCCAGAGGATTCAGGCACAGGAAG
  GGGACTTTCCCATGCCTTTCATATCTGCCAAATCGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAAT
  CCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTACCGAGAG
  ATAGGCAGAAGACTGAAGTTTTGGAATGAGACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACA
  AGGCAGGGCGCTATCAGTGCCAATATAGGATAGGGCACTACAGGTTCCGGTACAGTGACACCCTGGAGCT
  GGTAGTGACAGGCTTGTATGGCAAACCCTTCCTCTCTGCAGATCGGGGTCTGGTGTTGATGCCAGGAGAG
  AATATTTCCCTCACGTGCAGCTCAGCACACATCCCATTTGATAGATTTTCACTGGCCAAGGAGGGAGAAC
  TTTCTCTGCCACAGCACCAAAGTGGGGAACACCCGGCCAACTTCTCTTTGGGTCCTGTGGACCTCAATGT
  CTCAGGGATCTACAGGTGCTACGACTCCATCCACCAAGATTACACGACGCAGAACTTGATCCGCATGGCC
  GTGGCAGGACTGGTCCTCGTGGCTCTCTTGGCCATACTGGTTGAAAATTGGCACAGCCATACGGCACTGA
  ACAAGGAAGCCTCGGCAGATGTGGCTGAACCGAGCTGGAGCCAACAGATGTGTCAGCCAGGATTGACCTT
  TGCACGAACACCAAGTGTCTGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222470 representing NM_133269
  Red=Cloning site Green=Tags(s)
  
  MDPKQTTLLCLVLCLGQRIQAQEGDFPMPFISAKSSPVIPLDGSVKIQCQAIREAYLTQLMIIKNSTYRE
  IGRRLKFWNETDPEFVIDHMDANKAGRYQCQYRIGHYRFRYSDTLELVVTGLYGKPFLSADRGLVLMPGE
  NISLTCSSAHIPFDRFSLAKEGELSLPQHQSGEHPANFSLGPVDLNVSGIYRCYDSIHQDYTTQNLIRMA
  VAGLVLVALLAILVENWHSHTALNKEASADVAEPSWSQQMCQPGLTFARTPSVCK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133269
• ORF Size: 795 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133269.1, NM_133269.2, NM_133269.3, NP_579803.1
• RefSeq Size: 1605 bp
• RefSeq ORF: 798 bp
• Locus ID: 2204
• Cytogenetics: 19q13.42
• Protein Families: Transmembrane
• MW: 27.3 kDa
• Gene Summary: 'This gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'