ANKS1B (NM_020140) Human Tagged ORF Clone

CAT#: RC222572

ANKS1B (Myc-DDK-tagged)-Human ankyrin repeat and sterile alpha motif domain containing 1B (ANKS1B), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY412629) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222572 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ANKS1B protein (Cat# TP322572). The protein was produced from HEK293T cells transfected with ANKS1B cDNA clone (Cat# RC222572) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKS1B
• Synonyms: AIDA; AIDA-1; ANKS2; cajalin-2; EB-1; EB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222572 representing NM_020140
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTGGCAATGCCACCTCTCAGCCCAGGACTACCGCTATTACCCCGTGGACGGCTACTCCCTGCTTA
  AACGCTTCCCTCTTCATCCTCTTACAGGACCCAGATGCCCTGTCCAAACAGTGGGACAATGGTTGGAAAG
  CATTGGGCTACCTCAGTACGAGAACCACCTGATGGCTAATGGATTTGACAATGTGCAGTTTATGGGAAGC
  AATGTTATGGAAGATCAGGATTTGTTGGAAATTGGAATCCTTAATTCTGGGCACAGACAAAGAATTCTAC
  AGGCAATCCAGCTCCTTCCAAAGATGAGACCCATTGGGCATGATGGCTACCATCCCACCTCTGTAGCTGA
  GTGGCTGGATTCCATTGAACTGGGCGACTACACCAAAGCCTTTCTAATTAATGGCTACACTTCGATGGAC
  CTGTTGAAAAAAATCTGGGAGGTTGAACTTATTAATGTTTTAAAAATCAATTTGATTGGCCACAGGAAAC
  GTATTTTGGCATCTCTGGGAGACAGGCTGCACGACGATCCCCCACAGAAGCCCCCTCGGTCCATCACCCT
  CAGGACAGGAGACTGGGGAGAACCTTCCATTACCTTGCGACCTCCGAATGAAGCCACAGCCTCTACCCCG
  GTACAGTACTGGCAGCATCACCCAGAAAAGCTTATCTTCCAGTCGTGTGATTACAAAGCTTTTTATTTAG
  GTTCTATGCTGATAAAAGAGCTTAGGGGGACAGAATCAACCCAAGATGCTTGTGCAAAAATGCGGGCTAA
  CTGTCAGAAGTCTACAGAGCAAATGAAGAAGGTCCCTACTATTATTCTTTCTGTCTCATATAAAGGAGTC
  AAATTTATTGATGCAACAAATAAGAACATAATTGCTGAGCATGAAATTCGTAATATCTCCTGTGCTGCCC
  AGGACCCAGAAGACCTCTCAACATTTGCCTATATCACAAAAGATTTGAAGTCTAATCACCACTACTGTCA
  TGTGTTTACTGCCTTTGATGTGAATTTAGCCTATGAAATCATCCTAACCCTGGGACAGGCATTCGAAGTC
  GCTTACCAGCTAGCACTACAAGCAAGAAAAGGGGGACACTCCTCCACACTTCCAGAAAGCTTTGAAAACA
  AACCCTCCAAACCCATCCCCAAGCCCCGCGTTAGCATTCGCAAGTCCGTGGATCTTCTTCATGCCAGCCA
  TACCGGGCAGGAACCATCAGAGAGACACACTGAGGAGGCACTCAGAAAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222572 representing NM_020140
  Red=Cloning site Green=Tags(s)
  
  MMWQCHLSAQDYRYYPVDGYSLLKRFPLHPLTGPRCPVQTVGQWLESIGLPQYENHLMANGFDNVQFMGS
  NVMEDQDLLEIGILNSGHRQRILQAIQLLPKMRPIGHDGYHPTSVAEWLDSIELGDYTKAFLINGYTSMD
  LLKKIWEVELINVLKINLIGHRKRILASLGDRLHDDPPQKPPRSITLRTGDWGEPSITLRPPNEATASTP
  VQYWQHHPEKLIFQSCDYKAFYLGSMLIKELRGTESTQDACAKMRANCQKSTEQMKKVPTIILSVSYKGV
  KFIDATNKNIIAEHEIRNISCAAQDPEDLSTFAYITKDLKSNHHYCHVFTAFDVNLAYEIILTLGQAFEV
  AYQLALQARKGGHSSTLPESFENKPSKPIPKPRVSIRKSVDLLHASHTGQEPSERHTEEALRKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020140
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020140.1, NM_020140.2, NM_020140.3, NP_064525.1
• RefSeq Size: 1714
• RefSeq ORF: 1245
• Locus ID: 56899
• Domains: SAM, PID
• MW: 46.9 kDa
• Gene Summary: This gene encodes a multi-domain protein that is predominantly expressed in brain and testis. This protein interacts with amyloid beta protein precursor (AbetaPP) and may have a role in normal brain development, and in the pathogenesis of Alzheimer's disease. Expression of this gene has been shown to be elevated in patients with pre-B cell acute lymphocytic leukemia associated with t(1;19) translocation. Alternatively spliced transcript variants encoding different isoforms (some with different subcellular localization, PMID:15004329) have been described for this gene. [provided by RefSeq, Aug 2011]