MAGEC3 (NM_177456) Human Tagged ORF Clone

CAT#: RC222612

MAGEC3 (Myc-DDK-tagged)-Human melanoma antigen family C, 3 (MAGEC3), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406172) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222612 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAGEC3
• Synonyms: CT7.2; HCA2; MAGE-C3; MAGEC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222612 representing NM_177456
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCTCTTTCCAAACCTTCCACGCCTCAGCTTTGAGGAAGACTTCCAGAACCCGAGTGTGACAGAGG
  ACTTGGTAGATGCACAGGATTCCATAGATGAGGAGGAGGAGGATGCCTCCTCCACTTCCTCTTCCTCTTT
  CCACTTTTTATTCCCCTCCTCCTCTTCCTTGTCCTCATCCTCACCCTTGTCCTCACCCTTACCCTCTACT
  CTCATTCTGGGTGTTCCAGAAGATGAGGATATGCCTGCTGCTGGGATGCCACCTCTTCCCCAGAGTCCTC
  CTGAGATTCCTCCCCAGGGTCCTCCCAAGATCTCTCCCCAGGGTCCTCCGCAGAGTCCTCCCCAGAGTCC
  TCTAGACTCCTGCTCATCCCCTCTTTTGTGGACCCGATTGGATGAGGAGTCCAGCAGTGAAGAGGAGGAT
  ACAGCTACTTGGCATGCCTTGCCAGAAAGTGAATCCTTGCCCAGGTATGCCCTGGATGAAAAGGTGGCTG
  AGTTGGTGCAGTTTCTTCTCCTCAAATATCAAACAAAAGAGCCTGTCACAAAGGCAGAGATGCTGACGAC
  TGTCATCAAGAAGTATAAGGACTATTTTCCCATGATCTTCGGGAAAGCCCATGAGTTCATAGAGCTAATT
  TTTGGCATTGCCCTGACTGATATGGACCCCGACAACCACTCCTATTTCTTTGAAGACACATTAGACCTCA
  CCTATGAGGGAAGCCTGATTGATGACCAGGGCATGCCCAAGAACTGTCTCCTGATTCTTATTCTCAGTAT
  GATCTTCATAAAGGGCAGCTGTGTCCCCGAGGAGGTCATCTGGGAAGTGTTGAGTGCAATAGGGGTGTGT
  GCTGGGAGGGAGCACTTTATATATGGGGATCCCAGAAAGCTGCTCACTATACATTGGGTGCAGAGAAAGT
  ACCTGGAGTACCGGGAGGTGCCCAACAGTGCTCCTCCACGTTATGAATTTTTGTGGGGTCCAAGAGCCCA
  TTCAGAGGCCAGCAAGAGAAGTCTTAGAGTTTTTATCCAAGCTATCCAGTATCATCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222612 representing NM_177456
  Red=Cloning site Green=Tags(s)
  
  MPLFPNLPRLSFEEDFQNPSVTEDLVDAQDSIDEEEEDASSTSSSSFHFLFPSSSSLSSSSPLSSPLPST
  LILGVPEDEDMPAAGMPPLPQSPPEIPPQGPPKISPQGPPQSPPQSPLDSCSSPLLWTRLDEESSSEEED
  TATWHALPESESLPRYALDEKVAELVQFLLLKYQTKEPVTKAEMLTTVIKKYKDYFPMIFGKAHEFIELI
  FGIALTDMDPDNHSYFFEDTLDLTYEGSLIDDQGMPKNCLLILILSMIFIKGSCVPEEVIWEVLSAIGVC
  AGREHFIYGDPRKLLTIHWVQRKYLEYREVPNSAPPRYEFLWGPRAHSEASKRSLRVFIQAIQYHP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177456
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_177456.1, NM_177456.2, NP_803251.1
• RefSeq Size: 1707
• RefSeq ORF: 1041
• Locus ID: 139081
• MW: 38.7 kDa
• Gene Summary: This gene is a member of the MAGEC gene family. The members of this family are not expressed in normal tissues, except for testis, and are expressed in tumors of various histological types. The MAGEC genes are clustered on chromosome Xq26-q27. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]