DEP1 (PTPRJ) (NM_002843) Human Tagged ORF Clone

CAT#: RC222707

PTPRJ (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, J (PTPRJ), transcript variant 1

Product Images

Coomassie blue staining of purified PTPRJ protein (Cat# TP322707). The protein was produced from HEK293T cells transfected with PTPRJ cDNA clone (Cat# RC222707) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTPRJ
• Synonyms: CD148; DEP1; HPTPeta; R-PTP-ETA; SCC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222707 representing NM_002843
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCGGCGGCGCGGGAGGCGCGGCTGCCTCCGCGCTCGCCCGGGCTGCGCTGGGCGCTGCCGCTGC
  TGCTGCTGCTGCTGCGCCTGGGCCAGATCCTGTGCGCAGGTGGCACCCCTAGTCCAATTCCTGACCCTTC
  AGTAGCAACTGTTGCCACAGGGGAAAATGGCATAACGCAGATCAGCAGTACAGCAGAATCCTTTCATAAA
  CAGAATGGAACTGGAACACCTCAGGTGGAAACAAACACCAGTGAGGATGGTGAAAGCTCTGGAGCCAACG
  ATAGTTTAAGAACACCTGAACAAGGATCTAATGGGACTGATGGGGCATCTCAAAAAACTCCCAGTAGCAC
  TGGGCCCAGTCCTGTGTTTGACATTAAAGCTGTTTCCATCAGTCCAACCAATGTGATCTTAACTTGGAAA
  AGTAATGACACAGCTGCTTCTGAGTACAAGTATGTAGTAAAGCATAAGATGGAAAATGAGAAGACAATTA
  CTGTTGTGCATCAACCATGGTGTAACATCACAGGCTTACGTCCAGCGACTTCATATGTATTCTCCATCAC
  TCCAGGAATAGGCAATGAGACTTGGGGAGATCCCAGAGTCATAAAAGTCATCACAGAGCCGATCCCAGTT
  TCTGATCTCCGTGTTGCCCTCACGGGTGTGAGGAAGGCTGCTCTCTCCTGGAGCAATGGCAATGGCACTG
  CCTCCTGCCGGGTTCTTCTTGAAAGCATTGGAAGCCATGAGGAGTTGACTCAAGACTCAAGACTTCAGGT
  CAATATCTCGGGCCTGAAGCCAGGGGTTCAATACAACATCAACCCGTATCTTCTACAATCAAATAAGACA
  AAGGGAGACCCCTTGGGCACAGAAGGTGGCTTGGATGCCAGCAATACAGAGAGAAGCCGGGCAGGGAGCC
  CCACCGCCCCTGTGCATGATGAGTCCCTCGTGGGACCTGTGGACCCATCCTCCGGCCAGCAGTCCCGAGA
  CACGGAAGTCCTGCTTGTCGGGTTAGAGCCTGGCACCCGATACAATGCCACCGTTTATTCCCAAGCAGCG
  AATGGCACAGAAGGACAGCCCCAGGCCATAGAGTTCAGGACAAATGCTATTCAGGTTTTTGACGTCACCG
  CTGTGAACATCAGTGCCACAAGCCTGACCCTGATCTGGAAAGTCAGCGATAACGAGTCGTCATCTAACTA
  TACCTACAAGATACATGTGGCGGGGGAGACAGATTCTTCCAATCTCAACGTCAGTGAGCCTCGCGCTGTC
  ATCCCCGGACTCCGCTCCAGCACCTTCTACAACATCACAGTGTGTCCTGTCCTAGGTGACATCGAGGGCA
  CGCCGGGCTTCCTCCAAGTGCACACCCCCCCTGTTCCAGTTTCTGACTTCCGAGTGACAGTGGTCAGCAC
  GACGGAGATCGGCTTAGCATGGAGCAGCCATGATGCAGAATCATTTCAGATGCATATCACACAGGAGGGA
  GCTGGCAATTCTCGGGTAGAAATAACCACCAACCAAAGTATTATCATTGGTGGCTTGTTCCCTGGAACCA
  AGTATTGCTTTGAAATAGTTCCAAAAGGACCAAATGGGACTGAAGGGGCATCTCGGACAGTTTGCAATAG
  AACTGTTCCCAGTGCAGTGTTTGACATCCACGTGGTCTACGTCACCACCACGGAGATGTGGCTGGACTGG
  AAGAGCCCTGACGGTGCTTCCGAGTATGTCTACCATTTAGTCATAGAGTCCAAGCATGGCTCTAACCACA
  CAAGCACGTATGACAAAGCGATTACTCTCCAGGGCCTGATTCCGGGCACCTTATATAACATCACCATCTC
  TCCAGAAGTGGACCACGTCTGGGGGGACCCCAACTCCACTGCACAGTACACACGGCCCAGCAATGTGTCC
  AACATTGATGTAAGTACCAACACCACAGCAGCAACTTTAAGTTGGCAGAACTTTGATGACGCCTCTCCCA
  CGTACTCCTACTGCCTTCTTATTGAGAAGGCTGGAAATTCCAGCAACGCAACACAAGTAGTCACGGACAT
  TGGAATTACTGACGCTACAGTCACTGAATTAATACCTGGCTCATCATACACAGTGGAGATCTTTGCACAA
  GTAGGGGATGGGATCAAGTCACTGGAACCTGGCCGGAAGTCATTCTGTACAGATCCTGCGTCCATGGCCT
  CCTTCGACTGCGAAGTGGTCCCCAAAGAGCCAGCCCTGGTTCTCAAATGGACCTGCCCTCCTGGCGCCAA
  TGCAGGCTTTGAGCTGGAGGTCAGCAGTGGAGCCTGGAACAATGCGACCCACCTGGAGAGCTGCTCCTCT
  GAGAATGGCACTGAGTATAGAACGGAAGTCACGTATTTGAATTTTTCTACCTCGTACAACATCAGCATCA
  CCACTGTGTCCTGTGGAAAGATGGCAGCCCCCACCCGGAACACCTGCACTACTGGCATCACAGATCCCCC
  TCCTCCAGATGGATCCCCTAATATTACATCTGTCAGTCACAATTCAGTAAAGGTCAAGTTCAGTGGATTT
  GAAGCCAGCCACGGACCCATCAAAGCCTATGCTGTCATTCTCACCACCGGGGAAGCTGGTCACCCTTCTG
  CAGATGTCCTGAAATACACGTATGACGATTTCAAAAAGGGAGCCTCAGATACTTATGTGACATACCTCAT
  AAGAACAGAAGAAAAGGGACGTTCTCAGAGCTTGTCTGAAGTTTTGAAATATGAAATTGACGTTGGGAAT
  GAGTCAACCACACTTGGTTATTACAATGGGAAGCTGGAACCTCTGGGCTCCTACCGGGCTTGTGTGGCTG
  GCTTCACCAACATTACCTTCCACCCTCAAAACAAGGGGCTCATTGATGGGGCTGAGAGCTATGTGTCCTT
  CAGTCGCTACTCAGATGCTGTTTCCTTGCCCCAGGATCCAGGTGTCATCTGTGGAGCGGTTTTTGGCTGT
  ATCTTTGGTGCCCTGGTTATTGTGACTGTGGGAGGCTTCATCTTCTGGAGAAAGAAGAGGAAAGATGCAA
  AGAATAATGAAGTGTCCTTTTCTCAAATTAAACCTAAAAAATCTAAGTTAATCAGAGTGGAGAATTTTGA
  GGCCTACTTCAAGAAGCAGCAAGCTGACTCCAACTGTGGGTTCGCAGAGGAATACGAAGATCTGAAGCTT
  GTTGGAATTAGTCAACCTAAATATGCAGCAGAACTGGCTGAGAATAGAGGAAAGAATCGCTATAATAATG
  TTCTGCCCTATGATATTTCCCGTGTCAAACTTTCGGTCCAGACCCATTCAACGGATGACTACATCAATGC
  CAACTACATGCCTGGCTACCACTCCAAGAAAGATTTTATTGCCACACAAGGACCTTTACCGAACACTTTG
  AAAGATTTTTGGCGTATGGTTTGGGAGAAAAATGTATATGCCATCATTATGTTGACTAAATGTGTTGAAC
  AGGGAAGAACCAAATGTGAGGAGTATTGGCCCTCCAAGCAGGCTCAGGACTATGGAGACATAACTGTGGC
  AATGACATCAGAAATTGTTCTTCCGGAATGGACCATCAGAGATTTCACAGTGAAAAATATCCAGACAAGT
  GAGAGTCACCCTCTGAGACAGTTCCATTTCACCTCCTGGCCAGACCACGGTGTTCCCGACACCACTGACC
  TGCTCATCAACTTCCGGTACCTCGTTCGTGACTACATGAAGCAGAGTCCTCCCGAATCGCCGATTCTGGT
  GCATTGCAGTGCTGGGGTCGGAAGGACGGGCACTTTCATTGCCATTGATCGTCTCATCTACCAGATAGAG
  AATGAGAACACCGTGGATGTGTATGGGATTGTGTATGACCTTCGAATGCATAGGCCTTTAATGGTGCAGA
  CAGAGGACCAGTATGTTTTCCTCAATCAGTGTGTTTTGGATATTGTCAGATCCCAGAAAGACTCAAAAGT
  AGATCTTATCTACCAGAACACAACTGCAATGACAATCTATGAAAACCTTGCGCCCGTGACCACATTTGGA
  AAGACCAATGGTTACATCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002843
• ORF Size: 4011 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002843.1, NM_002843.2, NM_002843.3, NP_002834.2
• RefSeq Size: 5119 bp
• RefSeq ORF: 4014 bp
• Locus ID: 5795
• Cytogenetics: 11p11.2
• Domains: Y_phosphatase, PTPc_motif, FN3
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Protein Pathways: Adherens junction
• MW: 145.93 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'