CELF2 (NM_001083591) Human Tagged ORF Clone

CAT#: RC222724

CELF2 (Myc-DDK-tagged)-Human CUGBP, Elav-like family member 2 (CELF2), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY421224) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222724 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CELF2 protein (Cat# TP322724). The protein was produced from HEK293T cells transfected with CELF2 cDNA clone (Cat# RC222724) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CELF2
• Synonyms: BRUNOL3; CELF-2; CUG-BP2; CUGBP2; ETR-3; ETR3; NAPOR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222724 representing NM_001083591
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGGAGCTTTGGATCACTCAGACCAACCAGACCCAGATGCCATTAAGATGTTTGTCGGACAGATCC
  CCCGGTCATGGTCGGAAAAGGAGCTGAAAGAACTTTTTGAGCCTTACGGAGCCGTCTACCAGATCAACGT
  CCTCCGGGACCGGAGTCAGAACCCTCCGCAGAGTAAAGGTTGTTGTTTCGTAACATTTTATACAAGAAAA
  GCTGCACTTGAGGCCCAGAATGCACTGCACAATATTAAAACTTTACCTGGGATGCATCATCCCATTCAGA
  TGAAACCTGCAGATAGTGAAAAGTCCAACGCTGTGGAAGACAGAAAATTGTTCATAGGAATGGTATCGAA
  GAAATGTAATGAGAACGACATCAGGGTGATGTTCTCTCCATTTGGCCAGATAGAAGAATGCCGGATCCTC
  CGGGGACCTGATGGGCTGAGTCGAGGCTGTGCGTTTGTCACATTTTCTACAAGGGCAATGGCACAGAATG
  CAATCAAAGCCATGCATCAGTCTCAGACCATGGAGGGCTGCTCTTCACCTATCGTGGTGAAGTTTGCTGA
  CACTCAGAAGGACAAAGAGCAAAGGCGCCTCCAGCAGCAGCTCGCTCAGCAGATGCAGCAGCTCAACACT
  GCCACCTGGGGGAACCTGACAGGGCTGGGCGGACTGACCCCACAGTATCTGGCGCTCCTGCAGCAGGCCA
  CCTCCTCCAGCAACCTGGGTGCGTTCAGCGGCATTCAACAAATGGCAGGCATGAATGCTTTACAGTTGCA
  GAACCTGGCGACGCTGGCTGCTGCTGCAGCTGCGGCCCAGACCTCAGCCACCAGCACCAATGCAAACCCT
  CTCTCTACCACGAGCAGCGCCCTGGGAGCCCTCACGAGTCCCGTGGCTGCTTCAACCCCCAACTCCACTG
  CTGGTGCAGCCATGAACTCCTTGACCTCTCTCGGGACTCTGCAAGGACTGGCTGGAGCCACTGTTGGACT
  GAATAATATTAATGCACTAGCAGGTACCATCAACAGTATGGCGGCTCTGAATGGAGGACTTGGCGCCACA
  GGCTTGACGAATGGCACGGCTGGCACCATGGACGCCCTCACCCAGGCCTACTCAGGAATTCAACAGTACG
  CAGCCGCCGCGCTGCCCACTCTGTACAGCCAGAGCCTGCTGCAGCAGCAGAGCGCTGCAGGCAGCCAGAA
  GGAAGGTCCAGAGGGGGCAAACCTCTTTATTTACCACCTTCCACAGGAATTTGGAGACCAGGACATTCTG
  CAGATGTTCATGCCTTTTGGAAATGTTATCTCTGCTAAAGTCTTCATTGACAAACAGACCAATCTGAGCA
  AGTGCTTTGGTTTTGTTAGCTACGACAATCCAGTCTCTGCACAAGCTGCTATCCAAGCTATGAATGGCTT
  TCAGATCGGCATGAAACGCTTGAAGGTGCAGCTGAAGCGTTCCAAAAACGACAGCAAACCTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222724 representing NM_001083591
  Red=Cloning site Green=Tags(s)
  
  MNGALDHSDQPDPDAIKMFVGQIPRSWSEKELKELFEPYGAVYQINVLRDRSQNPPQSKGCCFVTFYTRK
  AALEAQNALHNIKTLPGMHHPIQMKPADSEKSNAVEDRKLFIGMVSKKCNENDIRVMFSPFGQIEECRIL
  RGPDGLSRGCAFVTFSTRAMAQNAIKAMHQSQTMEGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNT
  ATWGNLTGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQLQNLATLAAAAAAAQTSATSTNANP
  LSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVGLNNINALAGTINSMAALNGGLGAT
  GLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGPEGANLFIYHLPQEFGDQDIL
  QMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQAMNGFQIGMKRLKVQLKRSKNDSKPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001083591
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001083591.1, NP_001077060.1
• RefSeq Size: 8053
• RefSeq ORF: 1467
• Locus ID: 10659
• MW: 51.9 kDa
• Gene Summary: Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]