KCNK10 (NM_021161) Human Tagged ORF Clone

CAT#: RC222775

KCNK10 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 10 (KCNK10), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412052) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222775 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNK10
• Synonyms: K2p10.1; PPP1R97; TREK-2; TREK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222775 representing NM_021161
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTTTCTCTACACAGACTTCTTTCTTTCCTTGGTGGCCGTTCCCGCAGCAGCACCGGTGTGCCAGC
  CCAAGAGCGCCACTAACGGGCAACCCCCGGCTCCGGCTCCGACTCCAACTCCGCGCCTGTCCATTTCCTC
  CCGAGCCACAGTGGTAGCCAGGATGGAAGGCACCTCCCAAGGGGGCTTGCAGACCGTCATGAAGTGGAAG
  ACGGTGGTTGCCATCTTTGTGGTTGTGGTGGTCTACCTTGTCACTGGCGGTCTTGTCTTCCGGGCATTGG
  AGCAGCCCTTTGAGAGCAGCCAGAAGAATACCATCGCCTTGGAGAAGGCGGAATTCCTGCGGGATCATGT
  CTGTGTGAGCCCCCAGGAGCTGGAGACGTTGATCCAGCATGCTCTTGATGCTGACAATGCGGGAGTCAGT
  CCAATAGGAAACTCTTCCAACAACAGCAGCCACTGGGACCTCGGCAGTGCCTTTTTCTTTGCTGGAACTG
  TCATTACGACCATAGGGTATGGGAATATTGCTCCGAGCACTGAAGGAGGCAAAATCTTTTGTATTTTATA
  TGCCATCTTTGGAATTCCACTCTTTGGTTTCTTATTGGCTGGAATTGGAGACCAACTTGGAACCATCTTT
  GGGAAAAGCATTGCAAGAGTGGAGAAGGTCTTTCGAAAAAAGCAAGTGAGTCAGACCAAGATCCGGGTCA
  TCTCAACCATCCTGTTCATCTTGGCCGGCTGCATTGTGTTTGTGACGATCCCTGCTGTCATCTTTAAGTA
  CATCGAGGGCTGGACGGCCTTGGAGTCCATTTACTTTGTGGTGGTCACTCTGACCACGGTGGGCTTTGGT
  GATTTTGTGGCAGGGGGAAACGCTGGCATCAATTATCGGGAGTGGTATAAGCCCCTAGTGTGGTTTTGGA
  TCCTTGTTGGCCTTGCCTACTTTGCAGCTGTCCTCAGTATGATCGGAGATTGGCTACGGGTTCTGTCCAA
  AAAGACAAAAGAAGAGGTGGGTGAAATCAAGGCCCATGCGGCAGAGTGGAAGGCCAATGTCACGGCTGAG
  TTCCGGGAGACACGGCGAAGGCTCAGCGTGGAGATCCACGATAAGCTGCAGCGGGCGGCCACCATCCGCA
  GCATGGAGCGCCGGCGGCTGGGCCTGGACCAGCGGGCCCACTCACTGGACATGCTGTCCCCCGAGAAGCG
  CTCTGTCTTTGCTGCCCTGGACACCGGCCGCTTCAAGGCCTCATCCCAGGAGAGCATCAACAACCGGCCC
  AACAACCTGCGCCTGAAGGGGCCGGAGCAGCTGAACAAGCATGGGCAGGGTGCGTCCGAGGACAACATCA
  TCAACAAGTTCGGGTCCACCTCCAGACTCACCAAGAGGAAAAACAAGGACCTCAAAAAGACCTTGCCCGA
  GGACGTTCAGAAAATCTACAAGACCTTCCGGAATTACTCCCTGGACGAGGAGAAGAAAGAGGAGGAGACG
  GAAAAGATGTGTAACTCAGACAACTCCAGCACAGCCATGCTGACGGACTGTATCCAGCAGCACGCTGAGT
  TGGAGAACGGAATGATACCCACGGACACCAAAGACCGGGAGCCGGAGAACAACTCATTACTTGAAGACAG
  AAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222775 representing NM_021161
  Red=Cloning site Green=Tags(s)
  
  MFFLYTDFFLSLVAVPAAAPVCQPKSATNGQPPAPAPTPTPRLSISSRATVVARMEGTSQGGLQTVMKWK
  TVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHVCVSPQELETLIQHALDADNAGVS
  PIGNSSNNSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIF
  GKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFG
  DFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKANVTAE
  FRETRRRLSVEIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQESINNRP
  NNLRLKGPEQLNKHGQGASEDNIINKFGSTSRLTKRKNKDLKKTLPEDVQKIYKTFRNYSLDEEKKEEET
  EKMCNSDNSSTAMLTDCIQQHAELENGMIPTDTKDREPENNSLLEDRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021161
• ORF Size: 1614 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021161.1, NM_021161.2, NM_021161.3, NM_021161.4, NP_066984.1
• RefSeq Size: 2710 bp
• RefSeq ORF: 1617 bp
• Locus ID: 54207
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 59.6 kDa
• Gene Summary: The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations, and is stimulated strongly by arachidonic acid and to a lesser degree by membrane stretching, intracellular acidification, and general anaesthetics. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Sep 2008]