PAX5 (NM_016734) Human Tagged ORF Clone
CAT#: RC222785L4
- LentiORF®
Lenti ORF clone of Human paired box 5 (PAX5), mGFP tagged
"NM_016734" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | PAX5 |
Synonyms | ALL3; BSAP |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC222785).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_016734 |
ORF Size | 1173 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016734.1, NP_057953.1 |
RefSeq Size | 3650 bp |
RefSeq ORF | 1176 bp |
Locus ID | 5079 |
UniProt ID | Q02548 |
Cytogenetics | 9p13.2 |
Protein Families | Transcription Factors |
MW | 42 kDa |
Gene Summary | 'This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC304450 | PAX5 (untagged)-Human paired box 5 (PAX5) |
USD 670.00 |
|
RC222785 | PAX5 (Myc-DDK-tagged)-Human paired box 5 (PAX5) |
USD 420.00 |
|
RG222785 | PAX5 (GFP-tagged) - Human paired box 5 (PAX5) |
USD 460.00 |
|
RC222785L1 | Lenti ORF clone of Human paired box 5 (PAX5), Myc-DDK-tagged |
USD 768.00 |
|
RC222785L2 | Lenti ORF clone of Human paired box 5 (PAX5), mGFP tagged |
USD 768.00 |
|
RC222785L3 | Lenti ORF clone of Human paired box 5 (PAX5), Myc-DDK-tagged |
USD 768.00 |
{0} Product Review(s)
Be the first one to submit a review