GALNT8 (NM_017417) Human Tagged ORF Clone

CAT#: RC222849

GALNT8 (Myc-DDK-tagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 8 (GalNAc-T8) (GALNT8)

Product Images

Western blot validation of overexpression lysate (Cat# LY413775) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222849 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GALNT8
• Synonyms: GALNAC-T8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222849 representing NM_017417
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTTTTGGAGGAAACTCCCCAAAGCCCTCTTCATTGGGCTGACTCTGGCCATTGCTGTCAATCTCC
  TTCTGGTATTTTCTAGCAAGGGGACTTTACAAAACCTGTTTACGGGTGGTCTCCACAGGGAGCTTCCTTT
  ACATCTGAATAAACGCTACGGGGCAGTGATAAAGAGACTCTCCCACTTGGAGGTGGAATTGCAGGATCTG
  AAAGAAAGTATGAAATTAGCTCTGAGGCAACAAGAAAATGTGAACAGCACACTGAAGAGGGCGAAAGATG
  AAGTACGCCCTCTTCTAAAGGCAATGGAAACCAAGGTGAATGAGACAAAGAAGCACAAAACCCAAATGAA
  ACTCTTCCCACACTCACAGCTTTTCAGGCAATGGGGCGAGGATCTTTCTGAGGCCCAGCAGAAGGCGGCC
  CAGGACCTCTTCCGGAAGTTTGGTTACAACGCGTACCTCAGCAACCAGCTGCCTCTCAATCGCACCATCC
  CCGACACGCGAGACTACAGATGTCTTCGGAAGACATATCCTTCCCAACTCCCATCCCTCAGTGTCATTCT
  CATATTCGTGAATGAAGCTCTGTCCATTATACAACGGGCCATCACCAGTATCATCAACCGGACGCCCTCT
  CGATTGTTGAAGGAAATCATCTTGGTGGATGATTTCAGCTCAAATGGAGAACTAAAGGTACACTTGGATG
  AGAAGATTAAGCTTTACAACCAGAAGTATCCAGGACTACTGAAAATAATACGGCATCCTGAAAGGAAAGG
  TCTTGCTCAAGCCCGCAACACTGGCTGGGAAGCTGCCACAGCAGACGTGGTCGCCATCTTGGATGCTCAC
  ATTGAAGTCAATGTTGGGTGGGCAGAGCCAATCTTGGCTCGGATTCAGGAGGACCGCACTGTGATTGTGT
  CTCCTGTGTTTGACAACATTCGTTTTGACACCTTCAAACTGGATAAGTATGAACTGGCAGTTGATGGGTT
  TAACTGGGAACTCTGGTGCCGCTACGATGCACTGCCACAAGCCTGGATTGATCTGCATGATGTCACTGCC
  CCAGTGAAGAGTCCTTCAATCATGGGCATCCTGGCTGCTAACAGGCACTTCCTGGGAGAGATCGGGTCTC
  TGGATGGTGGAATGCTCATCTATGGAGGAGAGAACGTGGAGCTTAGCCTGAGGGTGTGGCAGTGTGGAGG
  GAAGGTCGAGATTTTGCCCTGTTCCCGGATTGCCCACCTAGAGAGACACCACAAGCCCTACGCCTTGGAT
  CTCACCGCTGCCTTGAAGCGCAATGCTCTGCGAGTGGCCGAAATCTGGATGGATGAGCACAAACACATGG
  TCTACTTGGCCTGGAACATACCTCTCCAGAACTCTGGAATAGATTTTGGAGACGTTTCTTCCAGAATGGC
  ACTCCGGGAAAAACTGAAATGTAAAACTTTTGACTGGTACCTGAAAAATGTTTATCCACTCTTGAAGCCA
  CTCCACACCATCGTGGGCTATGGAAGAATGAAAAACCTATTGGATGAAAATGTCTGCTTGGATCAGGGAC
  CCGTTCCAGGCAACACCCCCATCATGTATTACTGCCATGAATTCAGCTCACAGAATGTCTACTATCACCT
  AACTGGGGAGCTCTATGTGGGACAACTGATTGCAGAGGCCAGTGCTAGTGATCGCTGCCTGACAGACCCT
  GGCAAGGCGGAGAAGCCCACCTTAGAACCATGCTCCAAGGCAGCTAAGAATAGACTGCATATATATTGGG
  ATTTTAAACCGGGAGGAGCTGTCATAAACAGAGATACCAAGCGGTGTCTGGAGATGAAGAAGGATCTTTT
  GGGTAGCCACGTGCTTGTGCTCCAGACCTGTAGCACGCAAGTGTGGGAAATCCAGCACACTGTCAGAGAC
  TGGGGTCAGACCAACAGCCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222849 representing NM_017417
  Red=Cloning site Green=Tags(s)
  
  MMFWRKLPKALFIGLTLAIAVNLLLVFSSKGTLQNLFTGGLHRELPLHLNKRYGAVIKRLSHLEVELQDL
  KESMKLALRQQENVNSTLKRAKDEVRPLLKAMETKVNETKKHKTQMKLFPHSQLFRQWGEDLSEAQQKAA
  QDLFRKFGYNAYLSNQLPLNRTIPDTRDYRCLRKTYPSQLPSLSVILIFVNEALSIIQRAITSIINRTPS
  RLLKEIILVDDFSSNGELKVHLDEKIKLYNQKYPGLLKIIRHPERKGLAQARNTGWEAATADVVAILDAH
  IEVNVGWAEPILARIQEDRTVIVSPVFDNIRFDTFKLDKYELAVDGFNWELWCRYDALPQAWIDLHDVTA
  PVKSPSIMGILAANRHFLGEIGSLDGGMLIYGGENVELSLRVWQCGGKVEILPCSRIAHLERHHKPYALD
  LTAALKRNALRVAEIWMDEHKHMVYLAWNIPLQNSGIDFGDVSSRMALREKLKCKTFDWYLKNVYPLLKP
  LHTIVGYGRMKNLLDENVCLDQGPVPGNTPIMYYCHEFSSQNVYYHLTGELYVGQLIAEASASDRCLTDP
  GKAEKPTLEPCSKAAKNRLHIYWDFKPGGAVINRDTKRCLEMKKDLLGSHVLVLQTCSTQVWEIQHTVRD
  WGQTNSQ
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017417
• ORF Size: 1911 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017417.1, NP_059113.1
• RefSeq Size: 2135
• RefSeq ORF: 1914
• Locus ID: 26290
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• MW: 72.7 kDa
• Gene Summary: This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. [provided by RefSeq, Jul 2008]