PREPL (NM_001042385) Human Tagged ORF Clone

CAT#: RC222870

PREPL (Myc-DDK-tagged)-Human prolyl endopeptidase-like (PREPL), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY420864) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222870 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PREPL
• Synonyms: CMS22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222870 representing NM_001042385
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAGAAGACCAAATTATTTCTCCAAGCTTTGAAGTATAGTATTCCTCACCTTGGAAAATGCATGC
  AGAAACAGCATTTGAATCACTATAACTTCGCTGATCATTGTTACAATAGAATAAAATTGAAAAAATATCA
  CCTAACCAAGTGTCTTCAGAATAAACCCAAGATATCAGAGTTAGCAAGAAACATCCCAAGTCGGAGCTTC
  TCATGTAAGGATCTTCAGCCTGTTAAACAAGAAAACGAAAAACCCCTTCCAGAAAACATGGATGCATTTG
  AAAAAGTGAGAACAAAATTAGAAACACAGCCACAAGAAGAATATGAAATCATCAATGTGGAAGTTAAACA
  TGGTGGTTTTGTTTATTACCAAGAAGGTTGTTGCTTGGTTCGTTCCAAAGATGAAGAAGCAGACAATGAT
  AATTATGAAGTTTTATTCAATTTGGAGGAACTTAAGTTAGACCAGCCCTTCATTGATTGTATCAGAGTTG
  CTCCAGATGAAAAATATGTGGCTGCCAAGATAAGAACTGAAGATTCTGAAGCATCTACCTGTGTAATTAT
  AAAGCTCAGCGATCAGCCCGTAATGGAAGCTTCTTTCCCGAATGTGTCCAGTTTTGAATGGGTAAAGGAC
  GAGGAAGATGAAGATGTTTTATTCTACACCTTCCAGAGGAACCTTCGCTGTCATGACGTATATCGAGCCA
  CTTTTGGTGATAACAAACGTAATGAACGCTTTTACACAGAAAAAGACCCAAGCTACTTTGTTTTCCTTTA
  TCTTACAAAAGACAGTCGTTTCCTCACCATAAATATTATGAACAAGACTACTTCTGAAGTGTGGTTGATA
  GATGGCCTGAGCCCTTGGGACCCACCAGTACTTATCCAGAAGCGAATACATGGGGTCCTTTACTATGTTG
  AACACAGAGATGATGAATTATACATTCTCACTAATGTTGGAGAACCTACAGAATTTAAGCTCCCTCCTTG
  GGCCTGTGGATTCATAATGGATACAAATTCTGACCCAAAGAACTGCCCCTTTCAACTTTGCTCTCCAATA
  CGTCCCCCAAAATATTACACATACAAGTTTGCAGAAGGCAAACTGTTTGAGGAAACTGGGCATGAAGACC
  CAATCACAAAGACTAGTCGCGTTTTACGTCTAGAAGCCAAAAGCAAGGATGGAAAATTAGTGCCAATGAC
  TGTTTTCCACAAAACTGACTCTGAGGACTTGCAGAAGAAACCTCTCTTGGTACATGTATATGGAGCTTAT
  GGAATGGATTTGAAAATGAATTTCAGGCCTGAGAGGCGGGTCCTGGTGGATGATGGATGGATATTAGCAT
  ACTGCCATGTTCGAGGTGGTGGTGAGTTAGGCCTCCAGTGGCACGCTGATGGCCGCCTAACTAAAAAACT
  CAATGGCCTTGCTGATTTAGAGGCTTGCATTAAGACGCTTCATGGCCAAGGCTTTTCTCAGCCAAGTCTA
  ACAACCCTGACTGCTTTCAGTGCTGGAGGGGTGCTTGCAGGAGCATTGTGTAATTCTAATCCAGAGCTGG
  TGAGAGCGGTGACTTTGGAGGCACCTTTCTTGGATGTTCTCAACACCATGATGGACACTACACTTCCTCT
  GACATTAGAAGAATTAGAAGAATGGGGGAATCCTTCATCTGATGAAAAACACAAGAACTACATAAAACGT
  TACTGTCCCTATCAAAATATTAAACCTCAGCATTATCCTTCAATTCACATAACGGCATATGAAAACGATG
  AACGGGTACCTCTGAAAGGAATTGTAAGTTATACTGAGAAACTCAAGGAAGCCATCGCGGAGCATGCTAA
  GGACACAGGTGAAGGCTATCAGACCCCTAATATTATTCTAGATATTCAGCCTGGAGGCAATCATGTAATT
  GAGGATTCTCACAAAAAGATTACAGCCCAAATTAAATTCCTGTACGAGGAACTTGGACTTGACAGCACCA
  GTGTTTTCGAGGATCTTAAGAAATACCTGAAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222870 representing NM_001042385
  Red=Cloning site Green=Tags(s)
  
  MQQKTKLFLQALKYSIPHLGKCMQKQHLNHYNFADHCYNRIKLKKYHLTKCLQNKPKISELARNIPSRSF
  SCKDLQPVKQENEKPLPENMDAFEKVRTKLETQPQEEYEIINVEVKHGGFVYYQEGCCLVRSKDEEADND
  NYEVLFNLEELKLDQPFIDCIRVAPDEKYVAAKIRTEDSEASTCVIIKLSDQPVMEASFPNVSSFEWVKD
  EEDEDVLFYTFQRNLRCHDVYRATFGDNKRNERFYTEKDPSYFVFLYLTKDSRFLTINIMNKTTSEVWLI
  DGLSPWDPPVLIQKRIHGVLYYVEHRDDELYILTNVGEPTEFKLPPWACGFIMDTNSDPKNCPFQLCSPI
  RPPKYYTYKFAEGKLFEETGHEDPITKTSRVLRLEAKSKDGKLVPMTVFHKTDSEDLQKKPLLVHVYGAY
  GMDLKMNFRPERRVLVDDGWILAYCHVRGGGELGLQWHADGRLTKKLNGLADLEACIKTLHGQGFSQPSL
  TTLTAFSAGGVLAGALCNSNPELVRAVTLEAPFLDVLNTMMDTTLPLTLEELEEWGNPSSDEKHKNYIKR
  YCPYQNIKPQHYPSIHITAYENDERVPLKGIVSYTEKLKEAIAEHAKDTGEGYQTPNIILDIQPGGNHVI
  EDSHKKITAQIKFLYEELGLDSTSVFEDLKKYLKF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042385
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001042385.1, NM_001042385.2, NP_001035844.1
• RefSeq Size: 4636
• RefSeq ORF: 1998
• Locus ID: 9581
• Protein Families: Druggable Genome, Protease
• MW: 76.6 kDa
• Gene Summary: The protein encoded by this gene belongs to the prolyl oligopeptidase subfamily of serine peptidases. Mutations in this gene have been associated with hypotonia-cystinuria syndrome, also known as the 2p21 deletion syndrome. Several alternatively spliced transcript variants encoding either the same or different isoforms have been described for this gene. [provided by RefSeq, Jan 2010]