MST4 (STK26) (NM_001042452) Human Tagged ORF Clone

CAT#: RC222872

MST4 (Myc-DDK-tagged)-Human serine/threonine protein kinase MST4 (MST4), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY420912) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222872 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STK26
• Synonyms: MASK; MST4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222872 representing NM_001042452
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCACTCGCCGGTGGCTGTCCAAGTGCCTGGGATGCAGAATAACATAGCTGATCCAGAAGAACTGT
  TCACAAAATTAGAGCGCATTGGGAAAGGCTCATTTGGGGAAGTTTTCAAAGGAATTGATAACCGTACCCA
  GCAAGTCGTTGCTATTAAAATCATAGACCTTGAGGAAGCCGAAGATGAAATAGAAGACATTCAGCAAGAA
  ATAACTGTCTTGAGTCAATGTGACAGCTCATATGTAACAAAATACTATGGGTCATATTTAAAGGGGTCTA
  AATTATGGATAATAATGGAATACCTGGGCGGTGGTTCAGCACTGGATCTTCTTCGAGCTGGTCCATTTGA
  TGAGTTCCAGATTGCTACCATGCTAAAGGAAATTTTAAAAGGTCTGGACTATCTGCATTCAGAAAAGAAA
  ATTCACCGAGACATAAAAGCTGCCAATGTCTTGCTCTCAGAACAAGGAGATGTTAAACTTGCTGATTTTG
  GAGTTGCTGGTCAGCTGACAGATACACAGATTAAAAGAAATACCTTTGTGGGAACTCCATTTTGGATGGC
  TCCTGAAGTTATTCAACAGTCAGCTTATGACTCAAAACGTCCTACAGCAAAAGAACTTCTGAAACACAAA
  TTCATTGTAAAAAATTCAAAGAAGACTTCTTATCTGACTGAACTGATAGATCGTTTTAAGAGATGGAAGG
  CAGAAGGACACAGTGATGATGAATCTGATTCCGAGGGCTCTGATTCGGAATCTACCAGCAGGGAAAACAA
  TACTCATCCTGAATGGAGCTTTACCACCGTACGAAAGAAGCCTGATCCAAAGAAAGTACAGAATGGGGCA
  GAGCAAGATCTTGTGCAAACCCTGAGTTGTTTGTCTATGATAATCACACCTGCATTTGCTGAACTTAAAC
  AGCAGGACGAGAATAACGCTAGCAGGAATCAGGCGATTGAAGAACTCGAGAAAAGTATTGCTGTGGCTGA
  AGCCGCCTGTCCCGGCATCACAGATAAAATGGTGAAGAAACTAATTGAAAAATTTCAAAAGTGTTCAGCA
  GACGAATCCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222872 representing NM_001042452
  Red=Cloning site Green=Tags(s)
  
  MAHSPVAVQVPGMQNNIADPEELFTKLERIGKGSFGEVFKGIDNRTQQVVAIKIIDLEEAEDEIEDIQQE
  ITVLSQCDSSYVTKYYGSYLKGSKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDYLHSEKK
  IHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIQQSAYDSKRPTAKELLKHK
  FIVKNSKKTSYLTELIDRFKRWKAEGHSDDESDSEGSDSESTSRENNTHPEWSFTTVRKKPDPKKVQNGA
  EQDLVQTLSCLSMIITPAFAELKQQDENNASRNQAIEELEKSIAVAEAACPGITDKMVKKLIEKFQKCSA
  DESP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042452
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001042452.1, NP_001035917.1
• RefSeq Size: 2875
• RefSeq ORF: 1065
• Locus ID: 51765
• Protein Families: Druggable Genome, Protein Kinase
• MW: 39.5 kDa
• Gene Summary: The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]