Corticotropin Releasing Factor Receptor 2 (CRHR2) (NM_001883) Human Tagged ORF Clone
CAT#: RC222881
CRHR2 (Myc-DDK-tagged)-Human corticotropin releasing hormone receptor 2 (CRHR2), transcript variant 1
"NM_001883" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CRHR2 |
Synonyms | CRF-RB; CRF2; CRFR2; HM-CRF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC222881 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCGGCACTGCTCCACAGCCTGCTGGAGGCCAACTGCAGCCTGGCGCTGGCTGAAGAGCTGCTCT TGGACGGCTGGGGGCCACCCCTGGACCCCGAGGGTCCCTACTCCTACTGCAACACGACCTTGGACCAGAT CGGAACGTGCTGGCCCCGCAGCGCTGCCGGAGCCCTCGTGGAGAGGCCGTGCCCCGAGTACTTCAACGGC GTCAAGTACAACACGACCCGGAATGCCTATCGAGAATGCTTGGAGAATGGGACGTGGGCCTCAAAGATCA ACTACTCACAGTGTGAGCCCATTTTGGATGACAAGCAGAGGAAGTATGACCTGCACTACCGCATCGCCCT TGTCGTCAACTACCTGGGCCACTGCGTATCTGTGGCAGCCCTGGTGGCCGCCTTCCTGCTTTTCCTGGCC CTGCGGAGCATTCGCTGTCTGCGGAATGTGATTCACTGGAACCTCATCACCACCTTTATCCTGCGAAATG TCATGTGGTTCCTGCTGCAGCTCGTTGACCATGAAGTGCACGAGAGCAATGAGGTCTGGTGCCGCTGCAT CACCACCATCTTCAACTACTTCGTGGTGACCAACTTCTTCTGGATGTTTGTGGAAGGCTGCTACCTGCAC ACGGCCATTGTCATGACCTACTCCACTGAGCGCCTGCGCAAGTGCCTCTTCCTCTTCATCGGATGGTGCA TCCCCTTCCCCATCATCGTCGCCTGGGCCATCGGCAAGCTCTACTATGAGAATGAACAGTGCTGGTTTGG CAAGGAGCCTGGCGACCTGGTGGACTACATCTACCAAGGCCCCATCATTCTCGTGCTCCTGATCAATTTC GTATTTCTGTTCAACATCGTCAGGATCCTAATGACAAAGTTACGCGCGTCCACCACATCCGAGACAATCC AGTACAGGAAGGCAGTGAAGGCCACCCTGGTGCTCCTGCCCCTCCTGGGCATCACCTACATGCTCTTCTT CGTCAATCCCGGGGAGGACGACCTGTCACAGATCATGTTCATCTATTTCAACTCCTTCCTGCAGTCGTTC CAGGGTTTCTTCGTGTCTGTCTTCTACTGCTTCTTCAATGGAGAGGTGCGCTCAGCCGTGAGGAAGAGGT GGCACCGCTGGCAGGACCATCACTCCCTTCGAGTCCCCATGGCCCGGGCCATGTCCATCCCTACATCACC CACACGGATCAGCTTCCACAGCATCAAGCAGACGGCCGCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222881 protein sequence
Red=Cloning site Green=Tags(s) MDAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALVERPCPEYFNG VKYNTTRNAYRECLENGTWASKINYSQCEPILDDKQRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLA LRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESNEVWCRCITTIFNYFVVTNFFWMFVEGCYLH TAIVMTYSTERLRKCLFLFIGWCIPFPIIVAWAIGKLYYENEQCWFGKEPGDLVDYIYQGPIILVLLINF VFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSF QGFFVSVFYCFFNGEVRSAVRKRWHRWQDHHSLRVPMARAMSIPTSPTRISFHSIKQTAAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001883 |
ORF Size | 1233 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001883.1, NM_001883.2, NM_001883.3, NM_001883.4, NP_001874.2 |
RefSeq Size | 2998 bp |
RefSeq ORF | 1236 bp |
Locus ID | 1395 |
Cytogenetics | 7p14.3 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
MW | 47.7 kDa |
Gene Summary | 'The protein encoded by this gene belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge. Studies in mice suggest that this receptor maybe involved in mediating cardiovascular homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jan 2011]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC303088 | CRHR2 (untagged)-Human corticotropin releasing hormone receptor 2 (CRHR2), transcript variant 1 |
USD 700.00 |
|
RG222881 | CRHR2 (GFP-tagged) - Human corticotropin releasing hormone receptor 2 (CRHR2), transcript variant 1 |
USD 460.00 |
|
RC222881L1 | Lenti ORF clone of Human corticotropin releasing hormone receptor 2 (CRHR2), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC222881L2 | Lenti ORF clone of Human corticotropin releasing hormone receptor 2 (CRHR2), transcript variant 1, mGFP tagged |
USD 768.00 |
|
RC222881L3 | Lenti ORF clone of Human corticotropin releasing hormone receptor 2 (CRHR2), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC222881L4 | Lenti ORF clone of Human corticotropin releasing hormone receptor 2 (CRHR2), transcript variant 1, mGFP tagged |
USD 620.00 |
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