MYO6 (NM_004999) Human Tagged ORF Clone
CAT#: RC222917L3
- LentiORF®
Lenti-ORF clone of MYO6 (Myc-DDK-tagged)-Human myosin VI (MYO6)
"NM_004999" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MYO6 |
Synonyms | DFNA22; DFNB37 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC222917).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_004999 |
ORF Size | 3855 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004999.3, NP_004990.2, NP_004990.3 |
RefSeq Size | 8662 bp |
RefSeq ORF | 3858 bp |
Locus ID | 4646 |
Cytogenetics | 6q14.1 |
Domains | IQ, myosin_head |
MW | 149.2 kDa |
Gene Summary | 'This gene encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The protein consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC116993 | MYO6 (untagged)-Human myosin VI (MYO6) |
USD 1,100.00 |
|
RC222917 | MYO6 (Myc-DDK-tagged)-Human myosin VI (MYO6) |
USD 1,350.00 |
|
RG222917 | MYO6 (GFP-tagged) - Human myosin VI (MYO6) |
USD 1,490.00 |
|
RC222917L4 | Lenti-ORF clone of MYO6 (mGFP-tagged)-Human myosin VI (MYO6) |
USD 1,920.00 |
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