MIER1 (NM_001077704) Human Tagged ORF Clone

CAT#: RC223062

MIER1 (Myc-DDK-tagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1), transcript variant 6

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MIER1
• Synonyms: ER1; MI-ER1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223062 representing NM_001077704
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCATCTGTTGAATCTTCAAGTCCAGGAGGTTCAGCAACATCAGATGACCATGAATTTGATC
  CATCAGCTGACATGCTGGTTCATGATTTTGATGATGAACGAACATTAGAAGAGGAAGAAATGATGGAAGG
  AGAAACAAACTTCAGCTCTGAAATAGAAGATCTTGCAAGGGAAGGCGACATGCCAATTCATGAACTTCTC
  AGCCTTTATGGTTATGGTAGTACTGTTCGACTACCTGAAGAAGATGAGGAAGAGGAAGAAGAGGAAGAAG
  AAGGTGAAGATGATGAAGATGCTGATAATGATGACAACAGTGGCTGTAGTGGGGAAAATAAAGAGGAGAA
  TATAAAGGATTCATCAGGTCAGGAGGATGAAACTCAGTCTTCCAATGATGATCCATCACAATCTGTTGCT
  TCTCAAGATGCCCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAGTAGAAG
  AAGAATCTGAAGAAGATGAAGATTATATTCCATCAGAAGACTGGAAAAAGGAGATTATGGTGGGCTCCAT
  GTTTCAAGCAGAAATTCCAGTTGGCATTTGTAGATACAAAGAAAATGAAAAAGTATATGAAAATGATGAT
  CAGCTCCTGTGGGACCCTGAGTACTTACCAGAAGATAAAGTGATTATATTTCTTAAAGATGCATCTAGAA
  GAACAGGTGATGAGAAGGGTGTAGAAGCAATTCCTGAAGGATCTCACATAAAAGACAATGAACAGGCTTT
  ATATGAATTGGTTAAATGCAATTTTGATACAGAAGAAGCATTGAGAAGATTAAGATTTAATGTAAAAGCA
  GCTAGAGAGGAATTATCTGTTTGGACAGAGGAAGAGTGTAGAAATTTTGAACAAGGGCTGAAGGCCTATG
  GAAAGGATTTTCATTTGATTCAGGCTAATAAAGTCCGAACAAGGTCAGTTGGTGAATGTGTAGCATTCTA
  TTACATGTGGAAAAAATCTGAACGTTATGATTTCTTTGCTCAGCAAACACGATTTGGAAAGAAGAAATAT
  AATCTTCATCCTGGTGTAACGGATTACATGGATCGTCTTCTAGACGAAAGTGAAAGTGCTGCATCTAGTC
  GAGCACCATCCCCTCCCCCAACTGCATCAAACAGTAGTAACAGCCAGTCTGAGAAAGAAGATGGCACTGT
  AAGCACTGCTAATCAAAATGGAGTGTCATCTAATGGACCAGGCATACTCCAAATGCTTCTTCCAGTTCAT
  TTTTCAGCCATCAGTTCAAGAGCCAATGCCTTTTTAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223062 representing NM_001077704
  Red=Cloning site Green=Tags(s)
  
  MAEPSVESSSPGGSATSDDHEFDPSADMLVHDFDDERTLEEEEMMEGETNFSSEIEDLAREGDMPIHELL
  SLYGYGSTVRLPEEDEEEEEEEEEGEDDEDADNDDNSGCSGENKEENIKDSSGQEDETQSSNDDPSQSVA
  SQDAQEIIRPRRCKYFDTNSEVEEESEEDEDYIPSEDWKKEIMVGSMFQAEIPVGICRYKENEKVYENDD
  QLLWDPEYLPEDKVIIFLKDASRRTGDEKGVEAIPEGSHIKDNEQALYELVKCNFDTEEALRRLRFNVKA
  AREELSVWTEEECRNFEQGLKAYGKDFHLIQANKVRTRSVGECVAFYYMWKKSERYDFFAQQTRFGKKKY
  NLHPGVTDYMDRLLDESESAASSRAPSPPPTASNSSNSQSEKEDGTVSTANQNGVSSNGPGILQMLLPVH
  FSAISSRANAFLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077704
• ORF Size: 1299 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001077704.1, NM_001077704.2, NP_001071172.1
• RefSeq Size: 1648
• RefSeq ORF: 1302
• Locus ID: 57708
• MW: 48.9 kDa
• Gene Summary: This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal. [provided by RefSeq, May 2013]