PCDHA5 (NM_018908) Human Tagged ORF Clone

CAT#: RC223080

PCDHA5 (Myc-DDK-tagged)-Human protocadherin alpha 5 (PCDHA5), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412920) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223080 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHA5
• Synonyms: CNR6; CNRN6; CNRS6; CRNR6; PCDH-ALPHA5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223080 representing NM_018908
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTATATTCCCGGAGAGGAAGTCTGGGATCCCGGCTCCTGCTGCTCTGGCTTCTCCTTGCCTACTGGA
  AGGCAGGGAGCGGCCAGCTCCACTACTCGATCCCGGAGGAAGCCAAACACGGAACCTTCGTTGGCCGCAT
  CGCGCAGGACCTAGGGCTGGAGCTGGCGGAGCTGGTGCCGCGCCTGTTCCGGGTGGCGTCCAAGGGCCGC
  GGGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGGGAGGAGC
  TGTGCCGGCGGAGGGCGGAGTGCAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGTTTTCCA
  TGTGGAGGTGGCAGTGAAGGACATCAATGACAATCCGCCCAGGTTCTCCAGACAAGAACAAAGATTATTC
  ATTTTAGAGTCAAGAATGCCAGATTCGCGGTTTCCGCTAGAGGGCGCGTCGGATTTGGATATTGGAGCAA
  ATGCACAATTGAGATACAGGTTAAATCCAAACGAATATTTTGACTTAGATGTTAAAACAAATGAAGAAGA
  AACGAACTTTTTAGAGCTGGTTTTGAGGAAATCCTTAGATAGAGAAGAAACACAAGAACACCGTTTATTA
  GTGATTGCAACTGATGGAGGAAAACCCGAACTAACAGGTACAGTTCAGTTGTTGATCAATGTATTGGATG
  CTAATGATAACGCCCCAGAATTTGATAAATCCATTTATAATGTCAGATTGTTGGAAAATGCACCAAGTGG
  GACATTAGTTATTAAACTGAACGCCTCAGATGCAGATGAGGGCATCAATAAGGAAATAGTGTATTTCTTT
  AGTAATCTTGTTCTTGACGATGTAAAGTCCAAATTTATAATTAATTCTAATACTGGTGAAATAAAAGTTA
  ACGGGGAACTGGATTATGAAGACTATAACTCATATGAAATTAATATTGATGCCATGGATAAAAGTACATT
  CCCATTATCAGGACACTGTAAAGTAGTAGTGAAACTCCTGGATGTGAATGATAATACCCCAGAGATGGCC
  ATAACCACCCTTTTCCTGCCTGTCAAAGAGGACGCTCCACTCAGCACGGTCATTGCTCTGATCAGCGTGT
  CTGACCGTGACTCAGGTGCCAACGGGCAGGTGACCTGCTCCCTAATGCCCCACGTTCCCTTCAAGCTGGT
  GTCCACCTTCAAGAATTACTACTCGTTGGTGCTGGACAGCGCCCTGGACCGCGAGAGCGTGTCGGTCTAT
  GAGCTGGTGGTGACCGCGCGGGACGGGGGCTCGCCTTCGCTGTGGGCCACCGCCAGCGTGTCTGTGGAAG
  TGGCCGACGTGAACGACAACGCTCCGGCGTTCGCGCAGCCCCAGTATACCGTGTTCGTGAAGGAGAACAA
  CCCGCCAGGCTGCCACATCTTCACGGTGTCTGCACGGGACGCGGACGCGCAGGAGAACGCCCTGGTGTCC
  TACTCGCTGGTGGAGCGGCGGGTGGGCGAGCGCCCGCTGTCGAGTTACGTTTCGGTGCACGCGGAGAGCG
  GCAAGGTGTACGCGCTGCAGCCGCTGGACCACGAGGAAGTGGAGCTGCTGCAGTTCCAGGTGAGCGCGCG
  CGACGCGGGCGTGCCGCCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAGAACGACAAC
  GCGCCGGCGCTGCTGGTGCCTCGAGTGGGTGGCACCGGCGGCGCAGTGAGCGAGCTGGTGCCGAGGTCAG
  TGGGTGCGGGCCACGTGGTGGCGAAGGTGCGCGCAGTGGACCCTGATTCGGGCTACAACGCTTGGCTTTC
  GTATGAGCTGCAGCCAGCGCCTGGCAGTGCGCGCATCCCGTTCCGCGTGGGGCTGTACACAGGCGAGATC
  AGCACAACACGCTCTCTGGATGAGACCGAAGCACCGCGCCACCGCCTTCTGGTGCTGGTGAAGGACCATG
  GAGAGCCCCCGCTGACAGCCACAGCCACAGTGCTGGTGTCGCTGGTGGAAAGTGGCCAGGCGCCGAAGGC
  CTCATCGCGGGCGTCGGCGGGCGCTGTGGGTCCCGAGGCTGCCCTGGTGGATGTCAACGTGTACCTGATC
  ATCGCCATCTGTGCGGTGTCCAGCCTGCTGGTGCTCACGCTGCTGCTGTACACCGCGCTGCGGTGCTCGG
  CGCAGCCCACCGAGGCCGTGTGCACACGGGGCAAGCCCACTCTGTTGTGCTCCAGCGCGGTGGGGAGCTG
  GTCGTACTCGCAGCAGAGGAGACAGAGGGTGTGCTCTGGGGAAGCTCCACCCAAAACAGACCTCATGGCC
  TTCAGTCCAAGCCTTCCTCAGGGTCCCACCTCTACAGACAACCCACGACAGCCCAACCCTGACTGGCGTT
  ACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGTGCACCTAGAGGAGGCTGGCATTCTACGGGCTGG
  TCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAGAACCAGAGGCAGGAGAAGTG
  TCCCCTCCAGTCGGTGCGGGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCAAAC
  AATCCGGTCCCGGTGAGTTGCCCGACAAATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCGGCA
  GGAGCCTACTAACAGCCAAATTGACAAAAGTGACTTCATAACCTTCGGCAAAAAGGAGGAGACCAAGAAA
  AAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAAGGGAACAGCACGACTGACAACA
  GTGACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223080 representing NM_018908
  Red=Cloning site Green=Tags(s)
  
  MVYSRRGSLGSRLLLLWLLLAYWKAGSGQLHYSIPEEAKHGTFVGRIAQDLGLELAELVPRLFRVASKGR
  GDLLEVNLQNGILFVNSRIDREELCRRRAECSIHLEVIVDRPLQVFHVEVAVKDINDNPPRFSRQEQRLF
  ILESRMPDSRFPLEGASDLDIGANAQLRYRLNPNEYFDLDVKTNEEETNFLELVLRKSLDREETQEHRLL
  VIATDGGKPELTGTVQLLINVLDANDNAPEFDKSIYNVRLLENAPSGTLVIKLNASDADEGINKEIVYFF
  SNLVLDDVKSKFIINSNTGEIKVNGELDYEDYNSYEINIDAMDKSTFPLSGHCKVVVKLLDVNDNTPEMA
  ITTLFLPVKEDAPLSTVIALISVSDRDSGANGQVTCSLMPHVPFKLVSTFKNYYSLVLDSALDRESVSVY
  ELVVTARDGGSPSLWATASVSVEVADVNDNAPAFAQPQYTVFVKENNPPGCHIFTVSARDADAQENALVS
  YSLVERRVGERPLSSYVSVHAESGKVYALQPLDHEEVELLQFQVSARDAGVPPLGSNVTLQVFVLDENDN
  APALLVPRVGGTGGAVSELVPRSVGAGHVVAKVRAVDPDSGYNAWLSYELQPAPGSARIPFRVGLYTGEI
  STTRSLDETEAPRHRLLVLVKDHGEPPLTATATVLVSLVESGQAPKASSRASAGAVGPEAALVDVNVYLI
  IAICAVSSLLVLTLLLYTALRCSAQPTEAVCTRGKPTLLCSSAVGSWSYSQQRRQRVCSGEAPPKTDLMA
  FSPSLPQGPTSTDNPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGEV
  SPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDKSDFITFGKKEETKK
  KKKKKKGNKTQEKKEKGNSTTDNSDQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018908
• ORF Size: 2808 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018908.1, NM_018908.2, NP_061731.1
• RefSeq Size: 5218
• RefSeq ORF: 2811
• Locus ID: 56143
• Domains: CA
• Protein Families: Transmembrane
• MW: 98.8 kDa
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]