SEC61A2 (NM_018144) Human Tagged ORF Clone

CAT#: RC223091

SEC61A2 (Myc-DDK-tagged)-Human Sec61 alpha 2 subunit (S. cerevisiae) (SEC61A2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEC61A2
• Synonyms: FLJ10578
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223091 representing NM_018144
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATCAAATTTTTAGAAGTTATCAAACCATTCTGTGCAGTTCTACCAGAAATTCAGAAACCGGAAA
  GGAAAATCCAGTTTAGAGAGAAGGTTCTGTGGACTGCTATAACGCTCTTCATTTTCTTAGTGTGTTGTCA
  GATCCCACTGTTTGGAATCATGTCATCAGATTCTGCAGATCCTTTCTACTGGATGAGAGTTATTCTGGCT
  TCCAATAGAGGAACTTTAATGGAATTGGGTATCTCCCCAATTGTAACATCTGGTTTGATTATGCAGTTGT
  TAGCTGGAGCCAAAATCATTGAAGTTGGAGATACACCGAAAGATAGAGCTTTATTCAATGGAGCCCAGAA
  ACTGTTTGGTATGATCATTACCATTGGGCAAGCCATTGTGTATGTCATGACGGGGATGTATGGGGACCCT
  GCAGAAATGGGTGCCGGAATCTGTCTCCTGATCATCATTCAGTTGTTTGTTGCTGGTTTGATTGTGCTGC
  TGTTAGATGAGCTGCTACAGAAGGGTTACGGCTTGGGGTCTGGGATTTCCCTCTTTATTGCCACCAACAT
  CTGTGAGACCATTGTCTGGAAGGCCTTTAGTCCCACTACCATTAACACTGGCAGAGGTACTGAGTTTGAG
  GGTGCAGTCATAGCTCTGTTCCATTTGTTGGCCACCAGGACGGACAAAGTCCGAGCTTTACGGGAGGCTT
  TTTATCGGCAGAACTTACCCAATCTCATGAACCTCATTGCTACAGTTTTTGTGTTTGCTGTTGTTATATA
  TTTCCAAGGATTTCGCGTTGATCTGCCCATTAAGTCGGCCCGTTACCGAGGACAGTACAGCAGCTACCCC
  ATCAAACTCTTCTACACCTCCAACATCCCCATCATCCTCCAGTCGGCCCTGGTGTCCAACCTGTATGTTA
  TTTCCCAGATGCTGTCTGTTCGATTTAGTGGCAACTTTTTAGTAAATTTACTAGGACAGTGGGCCGATGT
  CAGTGGGGGAGGACCCGCACGTTCTTACCCAGTTGGAGGCCTTTGTTACTATCTTTCTCCTCCTGAGTCC
  ATGGGCGCCATCTTTGAGGATCCTGTCCATGTCGTTGTTTATATCATCTTCATGTTGGGGTCATGTGCAT
  TCTTCTCTAAGACATGGATTGAAGTGTCTGGTTCCTCAGCCAAAGATGTAGCTAAACAGCTGAAAGAACA
  GCAGATGGTAATGAGGGGCCACCGAGATACCTCTATGGTTCATGAGCTTAATAGGTACATCCCCACCGCA
  GCTGCGTTTGGCGGTTTGTGCATTGGCGCCCTGTCAGTGCTGGCTGACTTCCTGGGGGCCATTGGATCTG
  GCACTGGAATTCTGCTAGCAGTCACTATTATTTACCAGTATTTTGAAATATTTGTTAAAGAACAGGCCGA
  AGTTGGTGGGATGGGTGCTTTGTTTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223091 representing NM_018144
  Red=Cloning site Green=Tags(s)
  
  MGIKFLEVIKPFCAVLPEIQKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPFYWMRVILA
  SNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMIITIGQAIVYVMTGMYGDP
  AEMGAGICLLIIIQLFVAGLIVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTINTGRGTEFE
  GAVIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYP
  IKLFYTSNIPIILQSALVSNLYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPES
  MGAIFEDPVHVVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNRYIPTA
  AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQAEVGGMGALFF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018144
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018144.2, NM_018144.3, NP_060614.2
• RefSeq Size: 1892 bp
• RefSeq ORF: 1431 bp
• Locus ID: 55176
• Domains: secY
• Protein Families: Transmembrane
• Protein Pathways: Vibrio cholerae infection
• MW: 52.1 kDa
• Gene Summary: The protein encoded by this gene has similarity to a mouse protein which suggests a role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. It may also be required for the assembly of membrane and secretory proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]