Pirin (PIR) (NM_003662) Human Tagged ORF Clone
CAT#: RC223111
PIR (Myc-DDK-tagged)-Human pirin (iron-binding nuclear protein) (PIR), transcript variant 1
"NM_003662" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PIR |
Synonyms | OTTHUMP00000022961; OTTHUMP00000022962; pirin; pirin (iron-binding nuclear protein) |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223111 representing NM_003662
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCCTCCAAGAAAGTTACTCTCTCAGTGCTCAGCCGGGAGCAGTCGGAAGGGGTTGGAGCGAGGG TCCGGAGAAGCATTGGCAGACCCGAGTTAAAAAATCTGGATCCGTTTTTACTGTTTGATGAATTTAAAGG AGGTAGACCAGGAGGATTTCCTGATCATCCACATCGAGGTTTTGAAACAGTATCCTACCTCCTGGAAGGG GGCAGCATGGCCCATGAAGACTTCTGTGGACACACTGGTAAAATGAACCCAGGAGATTTGCAGTGGATGA CTGCGGGCCGGGGCATTCTGCACGCTGAGATGCCTTGCTCAGAGGAGCCAGCCCATGGCCTACAACTGTG GGTTAATTTGAGGAGCTCAGAGAAGATGGTGGAGCCTCAGTACCAGGAACTGAAAAGTGAAGAAATCCCT AAACCCAGTAAGGATGGTGTGACAGTTGCTGTCATTTCTGGAGAAGCCCTGGGAATAAAGTCCAAGGTTT ACACTCGCACACCAACCTTATATTTGGACTTCAAATTGGACCCAGGAGCCAAACATTCCCAACCTATCCC TAAAGGGTGGACAAGCTTCATTTACACGATATCTGGAGATGTGTATATTGGGCCCGATGATGCACAACAA AAAATAGAACCTCATCACACAGCAGTGCTTGGAGAAGGTGACAGTGTCCAAGTGGAGAACAAGGATCCCA AGAGAAGCCACTTTGTCTTAATTGCTGGGGAGCCATTAAGAGAACCAGTTATCCAACATGGTCCATTTGT GATGAACACCAATGAAGAGATTTCTCAAGCTATTCTTGATTTCAGAAACGCAAAAAATGGGTTTGAAAGG GCCAAAACCTGGAAATCAAAGATTGGGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223111 representing NM_003662
Red=Cloning site Green=Tags(s) MGSSKKVTLSVLSREQSEGVGARVRRSIGRPELKNLDPFLLFDEFKGGRPGGFPDHPHRGFETVSYLLEG GSMAHEDFCGHTGKMNPGDLQWMTAGRGILHAEMPCSEEPAHGLQLWVNLRSSEKMVEPQYQELKSEEIP KPSKDGVTVAVISGEALGIKSKVYTRTPTLYLDFKLDPGAKHSQPIPKGWTSFIYTISGDVYIGPDDAQQ KIEPHHTAVLGEGDSVQVENKDPKRSHFVLIAGEPLREPVIQHGPFVMNTNEEISQAILDFRNAKNGFER AKTWKSKIGN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003662 |
ORF Size | 870 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003662.1, NM_003662.2, NM_003662.3, NP_003653.1 |
RefSeq Size | 1316 bp |
RefSeq ORF | 873 bp |
Locus ID | 8544 |
Domains | DUF209 |
Protein Families | Transcription Factors |
MW | 31.9 kDa |
Gene Summary | This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC111077 | PIR (untagged)-Human pirin (iron-binding nuclear protein) (PIR), transcript variant 1 |
USD 310.00 |
|
RG223111 | PIR (GFP-tagged) - Human pirin (iron-binding nuclear protein) (PIR), transcript variant 1 |
USD 460.00 |
|
RC223111L1 | Lenti ORF clone of Human pirin (iron-binding nuclear protein) (PIR), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC223111L2 | Lenti ORF clone of Human pirin (iron-binding nuclear protein) (PIR), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC223111L3 | Lenti ORF clone of Human pirin (iron-binding nuclear protein) (PIR), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC223111L4 | Lenti ORF clone of Human pirin (iron-binding nuclear protein) (PIR), transcript variant 1, mGFP tagged |
USD 620.00 |
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