PPA2 (NM_176869) Human Tagged ORF Clone
CAT#: RC223311
PPA2 (Myc-DDK-tagged)-Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1
"NM_176869" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PPA2 |
Synonyms | HSPC124; SCFAI; SCFI; SID6-306 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223311 representing NM_176869
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCGCTGCTGCGGCTGCTGCGCACGGGTGCCCCAGCCGCTGCGTGCCTGCGGTTGGGGACCAGTG CAGGGACCGGGTCGCGCCGTGCTATGGCCCTGTACCACACTGAGGAGCGCGGCCAGCCCTGCTCGCAGAA TTACCGCCTCTTCTTTAAGAATGTAACTGGTCACTACATTTCCCCCTTTCATGATATTCCTCTGAAGGTG AACTCTAAAGAGGAAAATGGCATTCCTATGAAGAAAGCACGAAATGATGAATATGAGAATCTGTTTAATA TGATTGTAGAAATACCTCGGTGGACAAATGCTAAAATGGAGATTGCCACCAAGGAGCCAATGAATCCCAT TAAACAATATGTAAAGGATGGAAAGCTACGCTATGTGGCGAATATCTTCCCTTACAAGGGTTATATATGG AATTATGGTACCCTCCCTCAGACTTGGGAAGATCCCCATGAAAAAGATAAGAGCACGAACTGCTTTGGAG ATAATGATCCTATTGATGTTTGCGAAATAGGCTCAAAGATTCTTTCTTGTGGAGAAGTTATTCATGTGAA GATCCTTGGAATTTTGGCTCTTATTGATGAAGGTGAAACAGATTGGAAATTAATTGCTATCAATGCGAAT GATCCTGAAGCCTCAAAGTTTCATGATATTGATGATGTTAAGAAGTTCAAACCGGGTTACCTGGAAGCTA CTCTTAATTGGTTTAGATTATATAAGGTACCAGATGGAAAACCAGAAAACCAGTTTGCTTTTAATGGAGA ATTCAAAAACAAGGCTTTTGCTCTTGAAGTTATTAAATCCACTCATCAATGTTGGAAAGCATTGCTTATG AAGAACTGTAATGGAGGAGCTATAAATTGCACAAACGTGCAGATATCTGATAGCCCTTTCCGTTGCACTC AAGAGGAAGCAAGATCATTAGTTGAATCGGTATCATCTTCACCAAATAAAGAAAGTAATGAAGAAGAGCA AGTGTGGCACTTCCTTGGCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223311 representing NM_176869
Red=Cloning site Green=Tags(s) MSALLRLLRTGAPAAACLRLGTSAGTGSRRAMALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKV NSKEENGIPMKKARNDEYENLFNMIVEIPRWTNAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIW NYGTLPQTWEDPHEKDKSTNCFGDNDPIDVCEIGSKILSCGEVIHVKILGILALIDEGETDWKLIAINAN DPEASKFHDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLM KNCNGGAINCTNVQISDSPFRCTQEEARSLVESVSSSPNKESNEEEQVWHFLGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_176869 |
ORF Size | 1002 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_176869.1, NM_176869.2, NP_789845.1 |
RefSeq Size | 1682 |
RefSeq ORF | 1005 |
Locus ID | 27068 |
Protein Pathways | Oxidative phosphorylation |
MW | 34.8 kDa |
Gene Summary | The protein encoded by this gene is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC307076 | PPA2 (untagged)-Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 570.00 |
|
RG223311 | PPA2 (GFP-tagged) - Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 460.00 |
|
RC223311L1 | Lenti ORF clone of Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC223311L2 | Lenti ORF clone of Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC223311L3 | Lenti ORF clone of Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC223311L4 | Lenti ORF clone of Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
USD 620.00 |
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