Macrophage Scavenger Receptor I (MSR1) (NM_138716) Human Tagged ORF Clone

CAT#: RC223314

MSR1 (Myc-DDK-tagged)-Human macrophage scavenger receptor 1 (MSR1), transcript variant SR-AIII

Product Images

Western blot validation of overexpression lysate (Cat# LY408527) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223314 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MSR1 protein (Cat# TP323314). The protein was produced from HEK293T cells transfected with MSR1 cDNA clone (Cat# RC223314) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MSR1
• Synonyms: CD204; phSR1; phSR2; SCARA1; SR-A; SR-AI; SR-AII; SR-AIII; SRA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223314 representing NM_138716
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGTGGGATCACTTTCACAATCAACAGGAGGACACTGATAGCTGCTCCGAATCTGTGAAATTTG
  ATGCTCGCTCAATGACAGCTTTGCTTCCTCCGAATCCTAAAAACAGCCCTTCCCTTCAAGAGAAACTGAA
  GTCCTTCAAAGCTGCACTGATTGCCCTTTACCTCCTCGTGTTTGCAGTTCTCATCCCTCTCATTGGAATA
  GTGGCAGCTCAACTCCTGAAGTGGGAAACGAAGAATTGCTCAGTTAGTTCAACTAATGCAAATGATATAA
  CTCAAAGTCTCACGGGAAAAGGAAATGACAGCGAAGAGGAAATGAGATTTCAAGAAGTCTTTATGGAACA
  CATGAGCAACATGGAGAAGAGAATCCAGCATATTTTAGACATGGAAGCCAACCTCATGGACACAGAGCAT
  TTCCAAAATTTCAGCATGACAACTGATCAAAGATTTAATGACATTCTTCTGCAGCTAAGTACCTTGTTTT
  CCTCAGTCCAGGGACATGGGAATGCAATAGATGAAATCTCCAAGTCCTTAATAAGTTTGAATACCACATT
  GCTTGATTTGCAGCTCAACATAGAAAATCTGAATGGCAAAATCCAAGAGAATACCTTCAAACAACAAGAG
  GAAATCAGTAAATTAGAGGAGCGTGTTTACAATGTATCAGCAGAAATTATGGCTATGAAAGAAGAACAAG
  TGCATTTGGAACAGGAAATAAAAGGAGAAGTGAAAGTACTGAATAACATCACTAATGATCTCAGACTGAA
  AGATTGGGAACATTCTCAGACCTTGAGAAATATCACTTTAATTCAAGGTCCTCCTGGACCCCCGGGTGAA
  AAAGGAGATCGAGGTCCCACTGGAGAAAGTGGTCCACGAGGATTTCCAGGTCCAATAGGTCCTCCGGGTC
  TTAAAGGTGATCGGGGAGCAATTGGCTTTCCTGGAAGTCGAGGACTCCCAGGATATGCCGGAAGGCCAGG
  AAATTCTGGACCAAAAGGCCAGAAAGGGGAAAAGGGGAGTGGAAACACATTAAGTACTGGTCCAATATGG
  CTGAATGAAGTGTTTTGTTTTGGGAGAGAATCATCTATTGAAGAATGTAAAATTCGGCAATGGGGGACAA
  GAGCCTGTTCACATTCTGAAGATGCTGGAGTCACTTGCACTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223314 representing NM_138716
  Red=Cloning site Green=Tags(s)
  
  MEQWDHFHNQQEDTDSCSESVKFDARSMTALLPPNPKNSPSLQEKLKSFKAALIALYLLVFAVLIPLIGI
  VAAQLLKWETKNCSVSSTNANDITQSLTGKGNDSEEEMRFQEVFMEHMSNMEKRIQHILDMEANLMDTEH
  FQNFSMTTDQRFNDILLQLSTLFSSVQGHGNAIDEISKSLISLNTTLLDLQLNIENLNGKIQENTFKQQE
  EISKLEERVYNVSAEIMAMKEEQVHLEQEIKGEVKVLNNITNDLRLKDWEHSQTLRNITLIQGPPGPPGE
  KGDRGPTGESGPRGFPGPIGPPGLKGDRGAIGFPGSRGLPGYAGRPGNSGPKGQKGEKGSGNTLSTGPIW
  LNEVFCFGRESSIEECKIRQWGTRACSHSEDAGVTCTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138716
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138716.1, NM_138716.2, NP_619730.1
• RefSeq Size: 3493 bp
• RefSeq ORF: 1167 bp
• Locus ID: 4481
• Cytogenetics: 8p22
• Protein Families: Druggable Genome, Transmembrane
• MW: 42.8 kDa
• Gene Summary: 'This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008]'