KDM4B (NM_015015) Human Tagged ORF Clone

CAT#: RC223401

KDM4B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 4B (KDM4B)

Product Images

Western blot validation of overexpression lysate (Cat# LY414852) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223401 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KDM4B
• Synonyms: JMJD2B; TDRD14B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223401 representing NM_015015
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCTGAGGACCACGGCGCCCAGAACCCCAGCTGTAAAATCATGACGTTTCGCCCAACCATGGAAG
  AATTTAAAGACTTCAACAAATACGTGGCCTACATAGAGTCGCAGGGAGCCCACCGGGCGGGCCTGGCCAA
  GATCATCCCCCCGAAGGAGTGGAAGCCGCGGCAGACGTATGATGACATCGACGACGTGGTGATCCCGGCG
  CCCATCCAGCAGGTGGTGACGGGCCAGTCGGGCCTCTTCACGCAGTACAATATCCAGAAGAAGGCCATGA
  CAGTGGGCGAGTACCGCCGCCTGGCCAACAGCGAGAAGTACTGTACCCCGCGGCACCAGGACTTTGACGA
  CCTTGAACGCAAATACTGGAAGAACCTCACCTTTGTCTCCCCGATCTACGGGGCTGACATCAGCGGCTCT
  TTGTATGATGACGACGTGGCCCAGTGGAACATCGGGAGCCTCCGGACCATCCTGGACATGGTGGAGCGCG
  AGTGCGGCACCATCATCGAGGGCGTGAACACGCCCTACCTGTACTTCGGCATGTGGAAGACCACCTTCGC
  CTGGCACACCGAGGACATGGACCTGTACAGCATCAACTACCTGCACTTTGGGGAGCCTAAGTCCTGGTAC
  GCCATCCCACCAGAGCACGGCAAGCGCCTGGAGCGGCTGGCCATCGGCTTCTTCCCCGGGAGCTCGCAGG
  GCTGCGACGCCTTCCTGCGGCATAAGATGACCCTCATCTCGCCCATCATCCTGAAGAAGTACGGGATCCC
  CTTCAGCCGGATCACGCAGGAGGCCGGGGAATTCATGATCACATTTCCCTACGGCTACCACGCCGGCTTC
  AATCACGGGTTCAACTGCGCAGAATCTACCAACTTCGCCACCCTGCGGTGGATTGACTACGGCAAAGTGG
  CCACTCAGTGCACGTGCCGGAAGGACATGGTCAAGATCTCCATGGACGTGTTCGTGCGCATCCTGCAGCC
  CGAGCGCTACGAGCTGTGGAAGCAGGGCAAGGACCTCACGGTGCTGGACCACACGCGGCCCACGGCGCTC
  ACCAGCCCCGAGCTGAGCTCCTGGAGTGCGTCCCGGGCCTCGCTGAAGGCCAAGCTCCTCCGCAGGTCTC
  ACCGGAAACGGAGCCAGCCCAAGAAGCCGAAGCCCGAAGACCCCAAGTTCCCTGGGGAGGGTACGGCTGG
  GGCAGCGCTCCTAGAGGAGGCTGGGGGCAGCGTGAAGGAGGAGGCTGGGCCGGAGGTTGACCCCGAGGAG
  GAGGAGGAGGAGCCGCAGCCACTGCCACACGGCCGGGAGGCCGAGGGCGCAGAAGAGGACGGGAGGGGCA
  AGCTGCGGCCAACCAAGGCCAAGAGCGAGCGGAAGAAGAAGAGCTTCGGCCTGCTGCCCCCACAGCTGCC
  GCCCCCGCCTGCTCACTTCCCCTCAGAGGAGGCGCTGTGGCTGCCATCCCCACTGGAGCCCCCGGTGCTG
  GGCCCAGGCCCTGCAGCCATGGAGGAGAGCCCCCTGCCGGCACCCCTTAATGTCGTGCCCCCTGAGGTGC
  CCAGTGAGGAGCTAGAGGCCAAGCCTCGGCCCATCATCCCCATGCTGTACGTGGTGCCGCGGCCGGGCAA
  GGCAGCCTTCAACCAGGAGCACGTGTCCTGCCAGCAGGCCTTTGAGCACTTTGCCCAGAAGGGTCCGACC
  TGGAAGGAACCAGTTTCCCCCATGGAGCTGACGGGGCCAGAGGACGGTGCAGCCAGCAGTGGGGCAGGTC
  GCATGGAGACCAAAGCCCGGGCCGGAGAGGGGCAGGCACCGTCCACATTTTCCAAATTGAAGATGGAGAT
  CAAGAAGAGCCGGCGCCATCCCCTGGGCCGGCCGCCCACCCGGTCCCCACTGTCGGTGGTGAAGCAGGAG
  GCCTCAAGTGACGAGGAGGCATCCCCTTTCTCCGGGGAGGAAGATGTGAGTGACCCGGACGCCTTGAGGC
  CGCTGCTGTCTCTGCAGTGGAAGAACAGGGCGGCCAGCTTCCAGGCCGAGAGGAAGTTCAACGCAGCGGC
  TGCGCGCACGGAGCCCTACTGCGCCATCTGCACGCTCTTCTACCCCTACTGCCAGGCCCTACAGACTGAG
  AAGGAGGCACCCATAGCCTCCCTCGGAGAGGGCTGCCCGGCCACATTACCCTCCAAAAGCCGTCAGAAGA
  CCCGACCGCTCATCCCTGAGATGTGCTTCACCTCTGGCGGTGAGAACACGGAGCCGCTGCCTGCCAACTC
  CTACATCGGCGACGACGGGACCAGCCCCCTGATCGCCTGCGGCAAGTGCTGCCTGCAGGTCCATGCCAGT
  TGCTATGGCATCCGTCCCGAGCTGGTCAATGAAGGCTGGACGTGTTCCCGGTGCGCGGCCCACGCCTGGA
  CTGCGGAGTGCTGCCTGTGCAACCTGCGAGGAGGTGCGCTGCAGATGACCACCGATAGGAGGTGGATCCA
  CGTGATCTGTGCCATCGCAGTCCCCGAGGCGCGCTTCCTGAACGTGATTGAGCGCCACCCTGTGGACATC
  AGCGCCATCCCCGAGCAGCGGTGGAAGCTGAAATGCGTGTACTGCCGGAAGCGGATGAAGAAGGTGTCAG
  GTGCCTGTATCCAGTGCTCCTACGAGCACTGCTCCACGTCCTTCCACGTGACCTGCGCCCACGCCGCAGG
  CGTGCTCATGGAGCCGGACGACTGGCCCTATGTGGTCTCCATCACCTGCCTCAAGCACAAGTCGGGGGGT
  CACGCTGTCCAACTCCTGAGGGCCGTGTCCCTAGGCCAGGTGGTCATCACCAAGAACCGCAACGGGCTGT
  ACTACCGCTGTCGCGTCATCGGTGCCGCCTCGCAGACCTGCTACGAAGTGAACTTCGACGATGGCTCCTA
  CAGCGACAACCTGTACCCTGAGAGCATCACGAGTAGGGACTGTGTCCAGCTGGGACCCCCTTCCGAGGGG
  GAGCTGGTGGAGCTCCGGTGGACTGACGGCAACCTCTACAAGGCCAAGTTCATCTCCTCCGTCACCAGCC
  ACATCTACCAGGTGGAGTTTGAGGACGGGTCCCAGCTGACGGTGAAGCGTGGGGACATCTTCACCCTGGA
  GGAGGAGCTGCCCAAGAGGGTCCGCTCTCGGCTGTCACTGAGCACGGGGGCACCGCAGGAGCCCGCCTTC
  TCGGGGGAGGAGGCCAAGGCCGCCAAGCGCCCGCGTGTGGGCACCCCGCTTGCCACGGAGGACTCCGGGC
  GGAGCCAGGACTACGTGGCCTTCGTGGAGAGCCTCCTGCAGGTGCAGGGCCGGCCCGGAGCCCCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223401 representing NM_015015
  Red=Cloning site Green=Tags(s)
  
  MGSEDHGAQNPSCKIMTFRPTMEEFKDFNKYVAYIESQGAHRAGLAKIIPPKEWKPRQTYDDIDDVVIPA
  PIQQVVTGQSGLFTQYNIQKKAMTVGEYRRLANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGS
  LYDDDVAQWNIGSLRTILDMVERECGTIIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSWY
  AIPPEHGKRLERLAIGFFPGSSQGCDAFLRHKMTLISPIILKKYGIPFSRITQEAGEFMITFPYGYHAGF
  NHGFNCAESTNFATLRWIDYGKVATQCTCRKDMVKISMDVFVRILQPERYELWKQGKDLTVLDHTRPTAL
  TSPELSSWSASRASLKAKLLRRSHRKRSQPKKPKPEDPKFPGEGTAGAALLEEAGGSVKEEAGPEVDPEE
  EEEEPQPLPHGREAEGAEEDGRGKLRPTKAKSERKKKSFGLLPPQLPPPPAHFPSEEALWLPSPLEPPVL
  GPGPAAMEESPLPAPLNVVPPEVPSEELEAKPRPIIPMLYVVPRPGKAAFNQEHVSCQQAFEHFAQKGPT
  WKEPVSPMELTGPEDGAASSGAGRMETKARAGEGQAPSTFSKLKMEIKKSRRHPLGRPPTRSPLSVVKQE
  ASSDEEASPFSGEEDVSDPDALRPLLSLQWKNRAASFQAERKFNAAAARTEPYCAICTLFYPYCQALQTE
  KEAPIASLGEGCPATLPSKSRQKTRPLIPEMCFTSGGENTEPLPANSYIGDDGTSPLIACGKCCLQVHAS
  CYGIRPELVNEGWTCSRCAAHAWTAECCLCNLRGGALQMTTDRRWIHVICAIAVPEARFLNVIERHPVDI
  SAIPEQRWKLKCVYCRKRMKKVSGACIQCSYEHCSTSFHVTCAHAAGVLMEPDDWPYVVSITCLKHKSGG
  HAVQLLRAVSLGQVVITKNRNGLYYRCRVIGAASQTCYEVNFDDGSYSDNLYPESITSRDCVQLGPPSEG
  ELVELRWTDGNLYKAKFISSVTSHIYQVEFEDGSQLTVKRGDIFTLEEELPKRVRSRLSLSTGAPQEPAF
  SGEEAKAAKRPRVGTPLATEDSGRSQDYVAFVESLLQVQGRPGAPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015015
• ORF Size: 3288 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015015.1, NM_015015.2, NP_055830.1
• RefSeq Size: 5595
• RefSeq ORF: 3291
• Locus ID: 23030
• Protein Families: Druggable Genome
• MW: 121.7 kDa