RPH3AL (NM_006987) Human Tagged ORF Clone

CAT#: RC223419

RPH3AL (Myc-DDK-tagged)-Human rabphilin 3A-like (without C2 domains) (RPH3AL), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY416270) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223419 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPH3AL
• Synonyms: NOC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223419 representing NM_006987
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACACCATCTTCGGCAGCGGGAATGATCAGTGGGTTTGCCCCAATGACCGGCAGCTTGCCCTTC
  GAGCCAAGCTGCAGACGGGCTGGTCCGTGCACACCTACCAGACGGAGAAGCAGAGGAGGAAGCAGCACCT
  CAGCCCGGCGGAGGTGGAGGCCATCCTGCAGGTCATCCAGAGGGCAGAGCGGCTCGACGTCCTGGAGCAG
  CAGAGAATCGGGCGGCTGGTGGAGCGGCTGGAGACCATGAGGCGGAATGTGATGGGGAACGGCCTGTCCC
  AGTGTCTGCTCTGCGGGGAGGTGCTGGGCTTCCTGGGCAGCTCGTCGGTGTTCTGCAAAGACTGCAGGAA
  GAAAGTCTGCACCAAATGTGGGATCGAGGCCTCCCCTGGCCAGAAGCGGCCCCTGTGGCTGTGTAAGATC
  TGCAGTGAGCAAAGAGAGGTCTGGAAGAGGTCGGGGGCCTGGTTCTACAAAGGGCTCCCCAAGTATATCT
  TGCCCCTGAAGACCCCTGGCCGAGCTGATGACCCCCACTTCCGACCTTTGCCCACGGAACCGGCAGAGCG
  AGAGCCCAGAAGCTCTGAGACCAGCCGCATCTACACGTGGGCCCGAGGAAGAGTGGTTTCCAGTGACAGT
  GACAGTGACTCGGATCTTAGCTCCTCCAGCCTAGAGGACAGACTCCCATCCACTGGGGTCAGGGACCGGA
  AAGGCGACAAACCCTGGAAGGAGTCAGGTGGCAGCGTGGAGGCCCCCAGGATGGGGTTCACCCACCCGCC
  GGGCCACCTCTCTGGGTGCCAGAGCAGCCTGGCCAGTGGTGAGACGGGGACAGGCTCTGCTGACCCGCCA
  GGGGGACCCCGCCCCGGGCTGACCCGAAGGGCCCCGGTAAAAGACACACCTGGACGAGCCCCCGCTGCTG
  ACGCAGCTCCAGCAGGCCCCTCCAGCTGCCTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223419 representing NM_006987
  Red=Cloning site Green=Tags(s)
  
  MADTIFGSGNDQWVCPNDRQLALRAKLQTGWSVHTYQTEKQRRKQHLSPAEVEAILQVIQRAERLDVLEQ
  QRIGRLVERLETMRRNVMGNGLSQCLLCGEVLGFLGSSSVFCKDCRKKVCTKCGIEASPGQKRPLWLCKI
  CSEQREVWKRSGAWFYKGLPKYILPLKTPGRADDPHFRPLPTEPAEREPRSSETSRIYTWARGRVVSSDS
  DSDSDLSSSSLEDRLPSTGVRDRKGDKPWKESGGSVEAPRMGFTHPPGHLSGCQSSLASGETGTGSADPP
  GGPRPGLTRRAPVKDTPGRAPAADAAPAGPSSCLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006987
• ORF Size: 945 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006987.1, NM_006987.2, NM_006987.3, NP_008918.1
• RefSeq Size: 2610 bp
• RefSeq ORF: 948 bp
• Locus ID: 9501
• Domains: RPH3A_effector
• Protein Families: Secreted Protein
• MW: 34.3 kDa
• Gene Summary: The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jun 2010]