PPA2 (NM_001034191) Human Tagged ORF Clone

CAT#: RC223425

PPA2 (Myc-DDK-tagged)-Human pyrophosphatase (inorganic) 2 (PPA2), nuclear gene encoding mitochondrial protein, transcript variant 5

Product Images

Western blot validation of overexpression lysate (Cat# LY421959) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223425 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPA2
• Synonyms: FLJ20459; HSPC124; inorganic pyrophosphatase 2; MGC49850; pyrophosphatase (inorganic) 2; SID6-306
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223425 representing NM_001034191
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCGCTGCTGCGGCTGCTGCGCACGGGTGCCCCAGCCGCTGCGTGCCTGCGGTTGGGGACCAGTG
  CAGGGACCGGGTCGCGCCGTGCTATGGCCCTGTACCACACTGAGGAGCGCGGCCAGCCCTGCTCGCAGAA
  TTACCGCCTCTTCTTTAAGAATGTAACTGGTCACTACATTTCCCCCTTTCATGATATTCCTCTGAAGGTG
  AACTCTAAAGAGGAAAATGGCATTCCTATGAAGAAAGCACGAAATGATGAATATGAGATTGCCACCAAGG
  AGCCAATGAATCCCATTAAACAATATGTAAAGGATGGAAAGCTACGCTATGTGGCGAATATCTTCCCTTA
  CAAGGGTTATATATGGAATTATGGTACCCTCCCTCAGACTTGGGAAGATCCCCATGAAAAAGATAAGAGC
  ACGAACTGCTTTGGAGATAATGATCCTATTGATGTTTGCGAAATAGGCTCAAAGATTCTTTCTTGTGGAG
  AAGTTATTCATGTGAAGATCCTTGGAATTTTGGCTCTTATTGATGAAGGTGAAACAGATTGGAAATTAAT
  TGCTATCAATGCGAATGATCCTGAAGCCTCAAAGTTTCATGATATTGATGATGTTAAGAAGTTCAAACCG
  GGTTACCTGGAAGCTACTCTTAATTGGTTTAGATTATATAAGGTACCAGATGGAAAACCAGAAAACCAGT
  TTGCTTTTAATGGAGAATTCAAAAACAAGGCTTTTGCTCTTGAAGTTATTAAATCCACTCATCAATGTTG
  GAAAGCATTGCTTATGAAGAAGTGTAATGGAGGAGCTATAAATTGCACAAACGTGCAGATATCTGATAGC
  CCTTTCCGTTGCACTCAAGAGGAAGCAAGATCATTAGTTGAATCGGTATCATCTTCACCAAATAAAGAAA
  GTAATGAAGAAGAGCAAGTGTGGCACTTCCTTGGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223425 representing NM_001034191
  Red=Cloning site Green=Tags(s)
  
  MSALLRLLRTGAPAAACLRLGTSAGTGSRRAMALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKV
  NSKEENGIPMKKARNDEYEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIWNYGTLPQTWEDPHEKDKS
  TNCFGDNDPIDVCEIGSKILSCGEVIHVKILGILALIDEGETDWKLIAINANDPEASKFHDIDDVKKFKP
  GYLEATLNWFRLYKVPDGKPENQFAFNGEFKNKAFALEVIKSTHQCWKALLMKKCNGGAINCTNVQISDS
  PFRCTQEEARSLVESVSSSPNKESNEEEQVWHFLGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034191
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001034191.1, NP_001029363.1
• RefSeq Size: 1628
• RefSeq ORF: 950
• Locus ID: 27068
• Protein Pathways: Oxidative phosphorylation
• MW: 35.73 kDa
• Gene Summary: The protein encoded by this gene is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]