VNN1 (NM_004666) Human Tagged ORF Clone

CAT#: RC223462

VNN1 (Myc-DDK-tagged)-Human vanin 1 (VNN1)

Product Images

Western blot validation of overexpression lysate (Cat# LY401479) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223462 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VNN1
• Synonyms: HDLCQ8; Tiff66
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223462 representing NM_004666
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACTCAGTTGCCAGCTTACGTGGCAATTTTGCTTTTCTATGTCTCAAGAGCCAGCTGCCAGGACA
  CTTTCATTGCAGCTGTTTATGAGCATGCAGCGATATTGCCCAATGCCACCCTAACACCAGTGTCTCGTGA
  GGAGGCTTTGGCATTAATGAATCGGAATCTGGACATTTTGGAAGGAGCGATCACATCAGCAGCAGATCAG
  GGTGCGCATATTATTGTGACTCCAGAAGATGCTATTTATGGCTGGAACTTCAACAGGGACTCTCTCTACC
  CATATTTGGAGGACATCCCAGACCCTGAAGTAAACTGGATCCCCTGTAATAATCGTAACAGATTTGGCCA
  GACCCCAGTACAAGAAAGACTCAGCTGCCTGGCCAAGAACAACTCTATCTATGTTGTGGCAAATATTGGG
  GACAAGAAGCCATGCGATACCAGTGATCCTCAGTGTCCCCCTGATGGCCGTTACCAATACAACACTGATG
  TGGTATTTGATTCTCAAGGAAAACTGGTGGCACGCTACCATAAGCAAAACCTTTTCATGGGTGAAAATCA
  ATTCAATGTACCCAAGGAGCCTGAGATTGTGACTTTCAATACCACCTTTGGAAGTTTTGGCATTTTCACA
  TGCTTTGATATACTCTTCCATGATCCTGCTGTTACCTTGGTGAAAGATTTCCACGTGGACACCATAGTAT
  TCCCAACAGCTTGGATGAATGTTTTGCCACATTTGTCAGCTGTTGAATTCCACTCAGCTTGGGCTATGGG
  CATGAGGGTCAATTTCCTTGCATCCAACATACATTACCCCTCAAAGAAAATGACAGGAAGTGGCATCTAT
  GCACCCAATTCTTCAAGAGCATTTCATTATGATATGAAGACAGAAGAGGGAAAACTCCTCCTCTCGCAAC
  TGGATTCCCACCCATCCCATTCTGCAGTGGTGAACTGGACTTCCTATGCCAGCAGTATAGAAGCGCTCTC
  ATCAGGAAACAAGGAATTTAAAGGCACTGTCTTTTTCGATGAATTCACTTTTGTGAAGCTCACAGGAGTT
  GCAGGAAATTATACAGTTTGTCAGAAAGATCTCTGCTGTCATTTAAGCTACAAAATGTCTGAGAACATAC
  CAAATGAAGTGTACGCTCTAGGGGCATTTGACGGACTGCACACTGTGGAAGGGCGCTATTATCTACAGAT
  TTGTACCCTGTTGAAATGTAAAACGACTAATTTAAACACTTGCGGTGACTCAGCTGAAACAGCTTCTACC
  AGGTTTGAAATGTTCTCCCTCAGTGGCACTTTCGGAACCCAGTATGTCTTTCCTGAGGTGTTGCTGAGTG
  AAAATCAGCTTGCACCTGGAGAATTTCAGGTGTCAACTGACGGACGCTTGTTTAGTCTGAAGCCAACATC
  CGGACCTGTCTTAACAGTAACTCTGTTTGGGAGGTTGTATGAGAAGGACTGGGCATCAAATGCTTCATCA
  GGCCTCACAGCACAAGCAAGAATAATAATGCTAATAGTTATAGCACCTATTGTATGCTCATTAAGTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223462 representing NM_004666
  Red=Cloning site Green=Tags(s)
  
  MTTQLPAYVAILLFYVSRASCQDTFIAAVYEHAAILPNATLTPVSREEALALMNRNLDILEGAITSAADQ
  GAHIIVTPEDAIYGWNFNRDSLYPYLEDIPDPEVNWIPCNNRNRFGQTPVQERLSCLAKNNSIYVVANIG
  DKKPCDTSDPQCPPDGRYQYNTDVVFDSQGKLVARYHKQNLFMGENQFNVPKEPEIVTFNTTFGSFGIFT
  CFDILFHDPAVTLVKDFHVDTIVFPTAWMNVLPHLSAVEFHSAWAMGMRVNFLASNIHYPSKKMTGSGIY
  APNSSRAFHYDMKTEEGKLLLSQLDSHPSHSAVVNWTSYASSIEALSSGNKEFKGTVFFDEFTFVKLTGV
  AGNYTVCQKDLCCHLSYKMSENIPNEVYALGAFDGLHTVEGRYYLQICTLLKCKTTNLNTCGDSAETAST
  RFEMFSLSGTFGTQYVFPEVLLSENQLAPGEFQVSTDGRLFSLKPTSGPVLTVTLFGRLYEKDWASNASS
  GLTAQARIIMLIVIAPIVCSLSW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004666
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004666.1, NM_004666.2, NP_004657.1
• RefSeq Size: 3109 bp
• RefSeq ORF: 1542 bp
• Locus ID: 8876
• Protein Pathways: Pantothenate and CoA biosynthesis
• MW: 57.02 kDa
• Gene Summary: This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24. [provided by RefSeq, Feb 2009]