Olfactory receptor 13C8 (OR13C8) (NM_001004483) Human Tagged ORF Clone

CAT#: RC223507

OR13C8 (Myc-DDK-tagged)-Human olfactory receptor, family 13, subfamily C, member 8 (OR13C8)

Product Images

Western blot validation of overexpression lysate (Cat# LY423786) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223507 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified OR13C8 protein (Cat# TP323507). The protein was produced from HEK293T cells transfected with OR13C8 cDNA clone (Cat# RC223507) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR13C8
• Synonyms: OR9-10; OR37H
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223507 representing NM_001004483
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAGGACCAACGATTCCACGTCGACAGAATTTTTCCTGGTAGGGCTTTCTGCCCACCCAAAGCTCC
  AGACAGTTTTCTTCGTTCTAATTTTGTGGATGTACCTGATGATCCTGCTTGGAAATGGAGTCCTTATCTC
  AGTTATCATCTTTGATTCTCACCTGCACACCCCCATGTATTTCTTCCTCTGTAATCTTTCCTTCCTCGAC
  GTTTGCTACACAAGTTCCTCTGTCCCACTAATTCTTGCCAGCTTTCTGGCAGTAAAGAAAAAGGTTTCCT
  TCTCTGGGTGTATGGTGCAAATGTTTATTTCTTTTGCCATGGGGGCCACGGAGTGCATGATCTTAGGCAC
  GATGGCACTGGACCGCTATGTGGCCATCTGCTACCCACTGAGATACCCTGTCATCATGAGCAAGGGTGCC
  TATGTGGCCATGGCAGCTGGGTCCTGGGTCACTGGGCTTGTGGACTCAGTAGTGCAGACAGCTTTTGCAA
  TGCAGTTACCATTCTGTGCTAATAATGTCATTAAACATTTTGTCTGTGAAATTCTGGCTATCTTGAAACT
  GGCCTGTGCTGATATTTCAATCAATGTGATTAGTATGACAGGGTCGAATCTGATTGTTCTGGTTATTCCA
  TTGTTAGTAATTTCCATCTCTTACATATTTATTGTTGCCACTATTCTGAGGATTCCTTCCACTGAAGGAA
  AACATAAGGCCTTCTCCACCTGCTCAGCCCACCTGACAGTGGTGATTATATTCTATGGAACCATCTTCTT
  CATGTACGCAAAGCCTGAGTCTAAAGCCTCTGTTGATTCAGGTAATGAAGACATCATTGAGGCCCTCATC
  TCCCTTTTCTATGGAGTGATGACTCCCATGCTTAATCCTCTCATCTATAGTCTGCGAAACAAGGATGTAA
  AGGCTGCTGTCAAAAACATACTGTGTAGGAAAAACTTTTCTGATGGAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223507 representing NM_001004483
  Red=Cloning site Green=Tags(s)
  
  MERTNDSTSTEFFLVGLSAHPKLQTVFFVLILWMYLMILLGNGVLISVIIFDSHLHTPMYFFLCNLSFLD
  VCYTSSSVPLILASFLAVKKKVSFSGCMVQMFISFAMGATECMILGTMALDRYVAICYPLRYPVIMSKGA
  YVAMAAGSWVTGLVDSVVQTAFAMQLPFCANNVIKHFVCEILAILKLACADISINVISMTGSNLIVLVIP
  LLVISISYIFIVATILRIPSTEGKHKAFSTCSAHLTVVIIFYGTIFFMYAKPESKASVDSGNEDIIEALI
  SLFYGVMTPMLNPLIYSLRNKDVKAAVKNILCRKNFSDGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004483
• ORF Size: 960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004483.1, NP_001004483.1
• RefSeq Size: 963
• RefSeq ORF: 963
• Locus ID: 138802
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 35.1 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]